(Linear) (Six-base) MAPSORT of: KIBB7901.seq  Check: 582  from: 1  to: 327



KIBB7901.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     311     327 
   Size:         23     288      16

  Fragments arranged by size: 

                 288      23      16
 
AgeI A'CCGG_T

Cuts at:      0     118     327 
   Size:        118     209
 
AvrII C'CTAG_G

Cuts at:      0     238     327 
   Size:        238      89
 
BanII G_rGCy'C

Cuts at:      0     128     327 
   Size:        128     199
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     156     327 
   Size:        156     171
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     318     327 
   Size:        318       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     327 
   Size:        233      94
 
Bpu10I CC'TnA_GC

Cuts at:      0     278     327 
   Size:        278      49
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     132     327 
   Size:        132     195
 
BsaWI w'CCGG_w

Cuts at:      0     118     327 
   Size:        118     209
 
BseYI C'CCAG_C

Cuts at:      0      63     327 
   Size:         63     264
 
BsiEI CG_ry'CG

Cuts at:      0      90     327 
   Size:         90     237
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     327 
   Size:        128     199
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      63     327 
   Size:         63     264
 
BsrFI r'CCGG_y

Cuts at:      0     118     327 
   Size:        118     209
 
DraIII CAC_nnn'GTG

Cuts at:      0      13     327 
   Size:         13     314
 
EaeI y'GGCC_r

Cuts at:      0      87     223     247     327 
   Size:         87     136      24      80

  Fragments arranged by size: 

                 136      87      80      24
 
EagI C'GGCC_G

Cuts at:      0      87     327 
   Size:         87     240
 
EarI CTCTTCn'nnn_

Cuts at:      0      70     287     314     327 
   Size:         70     217      27      13

  Fragments arranged by size: 

                 217      70      27      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     233     311     327 
   Size:         23     210      78      16

  Fragments arranged by size: 

                 210      78      23      16
 
HaeII r_GCGC'y

Cuts at:      0      70     327 
   Size:         70     257
 
MscI TGG'CCA

Cuts at:      0     249     327 
   Size:        249      78
 
NotI GC'GGCC_GC

Cuts at:      0      87     327 
   Size:         87     240
 
PasI CC'CwG_GG

Cuts at:      0      45     143     327 
   Size:         45      98     184

  Fragments arranged by size: 

                 184      98      45
 
StyI C'CwwG_G

Cuts at:      0     238     327 
   Size:        238      89
 

 Enzymes that do cut:

     AcuI     AgeI    AvrII    BanII     BbsI     BplI     BpmI   Bpu10I
     BsaI    BsaWI    BseYI    BsiEI Bsp1286I    BspMI    BsrFI   DraIII
     EaeI     EagI     EarI  Eco57MI    HaeII     MscI     NotI     PasI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI