Restriction map for
pFN21AB7892
(Linear) (Six-base) MAPSORT of: KIBB7892.seq Check: 9307 from: 1 to: 1959
KIBB7892.orf
With 182 enzymes: SgfI *
July 3, 2012 16:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1657 1959
Size: 1657 302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1216 1959
Size: 613 603 743
Fragments arranged by size:
743 613 603
AleI CACnn'nnGTG
Cuts at: 0 1920 1959
Size: 1920 39
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1232 1938 1959
Size: 1232 706 21
Fragments arranged by size:
1232 706 21
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 539 571 1959
Size: 539 32 1388
Fragments arranged by size:
1388 539 32
AlwNI CAG_nnn'CTG
Cuts at: 0 520 1426 1663 1789 1959
Size: 520 906 237 126 170
Fragments arranged by size:
906 520 237 170 126
ApaI G_GGCC'C
Cuts at: 0 1382 1959
Size: 1382 577
ApaLI G'TGCA_C
Cuts at: 0 1670 1959
Size: 1670 289
ApoI r'AATT_y
Cuts at: 0 159 1129 1593 1959
Size: 159 970 464 366
Fragments arranged by size:
970 464 366 159
AseI AT'TA_AT
Cuts at: 0 1165 1959
Size: 1165 794
BamHI G'GATC_C
Cuts at: 0 1587 1959
Size: 1587 372
BanII G_rGCy'C
Cuts at: 0 114 1382 1806 1959
Size: 114 1268 424 153
Fragments arranged by size:
1268 424 153 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 516 1959
Size: 516 1443
BclI T'GATC_A
Cuts at: 0 765 887 1959
Size: 765 122 1072
Fragments arranged by size:
1072 765 122
BfrBI ATG'CAT
Cuts at: 0 276 1534 1959
Size: 276 1258 425
Fragments arranged by size:
1258 425 276
BglI GCCn_nnn'nGGC
Cuts at: 0 1422 1959
Size: 1422 537
BglII A'GATC_T
Cuts at: 0 289 362 1959
Size: 289 73 1597
Fragments arranged by size:
1597 289 73
BlpI GC'TnA_GC
Cuts at: 0 39 1959
Size: 39 1920
Bme1580I G_kGCm'C
Cuts at: 0 1382 1674 1959
Size: 1382 292 285
Fragments arranged by size:
1382 292 285
BmrI ACTGGGnnnn_n'
Cuts at: 0 1160 1339 1808 1959
Size: 1160 179 469 151
Fragments arranged by size:
1160 469 179 151
BmtI G_CTAG'C
Cuts at: 0 581 1959
Size: 581 1378
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1259 1959
Size: 1259 700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 965 1403 1484 1793 1895 1959
Size: 662 303 438 81 309 102 64
Fragments arranged by size:
662 438 309 303 102 81 64
Bpu10I CC'TnA_GC
Cuts at: 0 1574 1959
Size: 1574 385
BsaI GGTCTCn'nnnn_
Cuts at: 0 707 1959
Size: 707 1252
BsaBI GATnn'nnATC
Cuts at: 0 209 1959
Size: 209 1750
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 6 36 963 993 1788 1818 1959
Size: 6 30 927 30 795 30 141
Fragments arranged by size:
927 795 141 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 89 227 365 1478 1550 1796 1959
Size: 89 138 138 1113 72 246 163
Fragments arranged by size:
1113 246 163 138 138 89 72
BseYI C'CCAG_C
Cuts at: 0 1722 1959
Size: 1722 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1439 1853 1944 1959
Size: 1439 414 91 15
Fragments arranged by size:
1439 414 91 15
BsiHKAI G_wGCw'C
Cuts at: 0 1674 1959
Size: 1674 285
BsmI GAATG_Cn'
Cuts at: 0 920 1901 1959
Size: 920 981 58
Fragments arranged by size:
981 920 58
Bsp1286I G_dGCh'C
Cuts at: 0 114 1382 1674 1806 1959
Size: 114 1268 292 132 153
Fragments arranged by size:
1268 292 153 132 114
BspHI T'CATG_A
Cuts at: 0 566 1959
Size: 566 1393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1657 1916 1959
Size: 1657 259 43
Fragments arranged by size:
1657 259 43
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1663 1789 1959
Size: 1663 126 170
Fragments arranged by size:
1663 170 126
BstBI TT'CG_AA
Cuts at: 0 1026 1959
Size: 1026 933
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 1213 1959
Size: 744 469 746
Fragments arranged by size:
746 744 469
BstYI r'GATC_y
Cuts at: 0 289 362 1257 1587 1959
Size: 289 73 895 330 372
Fragments arranged by size:
895 372 330 289 73
BtgI C'CryG_G
Cuts at: 0 1374 1383 1401 1959
Size: 1374 9 18 558
Fragments arranged by size:
1374 558 18 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 551 1959
Size: 551 1408
BtsI GCAGTG_nn'
Cuts at: 0 100 1959
Size: 100 1859
DraIII CAC_nnn'GTG
Cuts at: 0 1117 1959
Size: 1117 842
DrdI GACnn_nn'nnGTC
Cuts at: 0 1067 1959
Size: 1067 892
EarI CTCTTCn'nnn_
Cuts at: 0 36 1071 1751 1959
Size: 36 1035 680 208
Fragments arranged by size:
1035 680 208 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 662 965 1216 1403 1484 1793 1895
Size: 613 49 303 251 187 81 309 102
Cuts at: 1895 1959
Size: 64
Fragments arranged by size:
613 309 303 251 187 102 81 64
49
EcoO109I rG'GnC_Cy
Cuts at: 0 434 976 1379 1396 1423 1959
Size: 434 542 403 17 27 536
Fragments arranged by size:
542 536 434 403 27 17
EcoRI G'AATT_C
Cuts at: 0 159 1959
Size: 159 1800
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 787 819 872 904 1788 1820 1959
Size: 787 32 53 32 884 32 139
Fragments arranged by size:
884 787 139 53 32 32 32
HincII GTy'rAC
Cuts at: 0 81 446 994 1959
Size: 81 365 548 965
Fragments arranged by size:
965 548 365 81
HpaI GTT'AAC
Cuts at: 0 81 446 1959
Size: 81 365 1513
Fragments arranged by size:
1513 365 81
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 1819 1959
Size: 312 1507 140
Fragments arranged by size:
1507 312 140
MslI CAynn'nnrTG
Cuts at: 0 536 1920 1959
Size: 536 1384 39
Fragments arranged by size:
1384 536 39
MspA1I CmG'CkG
Cuts at: 0 43 1726 1959
Size: 43 1683 233
Fragments arranged by size:
1683 233 43
NcoI C'CATG_G
Cuts at: 0 1374 1383 1401 1959
Size: 1374 9 18 558
Fragments arranged by size:
1374 558 18 9
NdeI CA'TA_TG
Cuts at: 0 1509 1959
Size: 1509 450
NheI G'CTAG_C
Cuts at: 0 577 1959
Size: 577 1382
NsiI A_TGCA'T
Cuts at: 0 278 1536 1959
Size: 278 1258 423
Fragments arranged by size:
1258 423 278
NspI r_CATG'y
Cuts at: 0 1000 1959
Size: 1000 959
PasI CC'CwG_GG
Cuts at: 0 1518 1890 1959
Size: 1518 372 69
Fragments arranged by size:
1518 372 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 520 940 1117 1426 1751 1959
Size: 520 420 177 309 325 208
Fragments arranged by size:
520 420 325 309 208 177
PfoI T'CCnGG_A
Cuts at: 0 1467 1959
Size: 1467 492
PpuMI rG'GwC_Cy
Cuts at: 0 434 976 1959
Size: 434 542 983
Fragments arranged by size:
983 542 434
PshAI GACnn'nnGTC
Cuts at: 0 989 1959
Size: 989 970
PsiI TTA'TAA
Cuts at: 0 1463 1959
Size: 1463 496
PspOMI G'GGCC_C
Cuts at: 0 1378 1959
Size: 1378 581
PstI C_TGCA'G
Cuts at: 0 107 1005 1668 1959
Size: 107 898 663 291
Fragments arranged by size:
898 663 291 107
PvuII CAG'CTG
Cuts at: 0 43 1726 1959
Size: 43 1683 233
Fragments arranged by size:
1683 233 43
SbfI CC_TGCA'GG
Cuts at: 0 1668 1959
Size: 1668 291
ScaI AGT'ACT
Cuts at: 0 1762 1959
Size: 1762 197
SexAI A'CCwGG_T
Cuts at: 0 1062 1113 1959
Size: 1062 51 846
Fragments arranged by size:
1062 846 51
SfcI C'TryA_G
Cuts at: 0 103 1001 1123 1664 1959
Size: 103 898 122 541 295
Fragments arranged by size:
898 541 295 122 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1422 1959
Size: 1422 537
StyI C'CwwG_G
Cuts at: 0 737 1374 1383 1401 1745 1959
Size: 737 637 9 18 344 214
Fragments arranged by size:
737 637 344 214 18 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1001 1959
Size: 1001 958
TatI w'GTAC_w
Cuts at: 0 1760 1959
Size: 1760 199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 535 1381 1408 1959
Size: 535 846 27 551
Fragments arranged by size:
846 551 535 27
Enzymes that do cut:
AarI AcuI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI BamHI BanII BbsI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraIII DrdI EarI Eco57MI EcoO109I EcoRI
Hin4I HincII HpaI MmeI MslI MspA1I NcoI NdeI
NheI NsiI NspI PasI PflMI PfoI PpuMI PshAI
PsiI PspOMI PstI PvuII SbfI ScaI SexAI SfcI
SfiI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AscI AsiSI AvaI AvrII BaeI BanI BbeI
BbvCI BcgI BciVI BmgBI BpuEI BsaAI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstEII BstZ17I Bsu36I ClaI CspCI DraI
EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
MscI NaeI NarI NgoMIV NotI NruI PacI PciI
PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI