Restriction map for
pFN21AB7883
(Linear) (Six-base) MAPSORT of: KIBB7883.seq Check: 2713 from: 1 to: 1737
KIBB7883.orf
With 182 enzymes: SgfI *
December 12, 2011 16:30 ..
AatII G_ACGT'C
Cuts at: 0 1253 1737
Size: 1253 484
AccI GT'mk_AC
Cuts at: 0 782 1737
Size: 782 955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1737
Size: 784 953
AfeI AGC'GCT
Cuts at: 0 1539 1737
Size: 1539 198
AflIII A'CryG_T
Cuts at: 0 1667 1737
Size: 1667 70
AgeI A'CCGG_T
Cuts at: 0 946 1737
Size: 946 791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 505 1737
Size: 505 1232
AlwNI CAG_nnn'CTG
Cuts at: 0 22 1390 1737
Size: 22 1368 347
Fragments arranged by size:
1368 347 22
ApaI G_GGCC'C
Cuts at: 0 223 289 361 367 1630 1737
Size: 223 66 72 6 1263 107
Fragments arranged by size:
1263 223 107 72 66 6
ApaLI G'TGCA_C
Cuts at: 0 942 1057 1737
Size: 942 115 680
Fragments arranged by size:
942 680 115
AvaI C'yCGr_G
Cuts at: 0 222 360 1623 1630 1719 1737
Size: 222 138 1263 7 89 18
Fragments arranged by size:
1263 222 138 89 18 7
BamHI G'GATC_C
Cuts at: 0 852 1737
Size: 852 885
BanI G'GyrC_C
Cuts at: 0 294 1405 1521 1558 1642 1737
Size: 294 1111 116 37 84 95
Fragments arranged by size:
1111 294 116 95 84 37
BanII G_rGCy'C
Cuts at: 0 223 289 361 367 506 872 962 1121
Size: 223 66 72 6 139 366 90 159
Cuts at: 1121 1433 1630 1737
Size: 312 197 107
Fragments arranged by size:
366 312 223 197 159 139 107 90
72 66 6
BbeI G_GCGC'C
Cuts at: 0 298 1737
Size: 298 1439
BbsI GAAGACnn'nnnn_
Cuts at: 0 1128 1737
Size: 1128 609
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1447 1481 1737
Size: 1447 34 256
Fragments arranged by size:
1447 256 34
BglI GCCn_nnn'nGGC
Cuts at: 0 332 1658 1737
Size: 332 1326 79
Fragments arranged by size:
1326 332 79
Bme1580I G_kGCm'C
Cuts at: 0 223 289 361 367 946 1061 1630 1737
Size: 223 66 72 6 579 115 569 107
Fragments arranged by size:
579 569 223 115 107 72 66 6
BmgBI CAC'GTC
Cuts at: 0 645 1056 1737
Size: 645 411 681
Fragments arranged by size:
681 645 411
BmrI ACTGGGnnnn_n'
Cuts at: 0 742 1737
Size: 742 995
BmtI G_CTAG'C
Cuts at: 0 1538 1737
Size: 1538 199
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 456 1737
Size: 456 1281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1126 1447 1737
Size: 1126 321 290
Fragments arranged by size:
1126 321 290
Bpu10I CC'TnA_GC
Cuts at: 0 268 1350 1737
Size: 268 1082 387
Fragments arranged by size:
1082 387 268
BsaI GGTCTCn'nnnn_
Cuts at: 0 1573 1737
Size: 1573 164
BsaAI yAC'GTr
Cuts at: 0 1187 1737
Size: 1187 550
BsaHI Gr'CG_yC
Cuts at: 0 295 1087 1250 1737
Size: 295 792 163 487
Fragments arranged by size:
792 487 295 163
BsaWI w'CCGG_w
Cuts at: 0 946 1737
Size: 946 791
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 970 1737
Size: 111 859 767
Fragments arranged by size:
859 767 111
BseYI C'CCAG_C
Cuts at: 0 93 300 348 754 1737
Size: 93 207 48 406 983
Fragments arranged by size:
983 406 207 93 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 591 1003 1569 1737
Size: 591 412 566 168
Fragments arranged by size:
591 566 412 168
BsiEI CG_ry'CG
Cuts at: 0 446 1737
Size: 446 1291
BsiHKAI G_wGCw'C
Cuts at: 0 151 274 506 946 962 1061 1737
Size: 151 123 232 440 16 99 676
Fragments arranged by size:
676 440 232 151 123 99 16
Bsp1286I G_dGCh'C
Cuts at: 0 151 223 274 289 361 367 506 872
Size: 151 72 51 15 72 6 139 366
Cuts at: 872 946 962 1061 1121 1433 1630 1737
Size: 74 16 99 60 312 197 107
Fragments arranged by size:
366 312 197 151 139 107 99 74
72 72 60 51 16 15 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1199 1517 1737
Size: 1199 318 220
Fragments arranged by size:
1199 318 220
BsrBI CCG'CTC
Cuts at: 0 440 720 1737
Size: 440 280 1017
Fragments arranged by size:
1017 440 280
BsrDI GCAATG_nn'
Cuts at: 0 1174 1737
Size: 1174 563
BsrFI r'CCGG_y
Cuts at: 0 78 445 946 1314 1737
Size: 78 367 501 368 423
Fragments arranged by size:
501 423 368 367 78
BssSI C'ACGA_G
Cuts at: 0 592 1737
Size: 592 1145
BstEII G'GTnAC_C
Cuts at: 0 276 1737
Size: 276 1461
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1656 1737
Size: 1656 81
BstYI r'GATC_y
Cuts at: 0 800 852 1737
Size: 800 52 885
Fragments arranged by size:
885 800 52
Bsu36I CC'TnA_GG
Cuts at: 0 102 1737
Size: 102 1635
BtgI C'CryG_G
Cuts at: 0 1281 1344 1568 1737
Size: 1281 63 224 169
Fragments arranged by size:
1281 224 169 63
EaeI y'GGCC_r
Cuts at: 0 42 443 1316 1737
Size: 42 401 873 421
Fragments arranged by size:
873 421 401 42
EagI C'GGCC_G
Cuts at: 0 443 1737
Size: 443 1294
EarI CTCTTCn'nnn_
Cuts at: 0 1498 1737
Size: 1498 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1317 1737
Size: 1317 420
EcoICRI GAG'CTC
Cuts at: 0 504 960 1737
Size: 504 456 777
Fragments arranged by size:
777 504 456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1126 1447 1737
Size: 784 342 321 290
Fragments arranged by size:
784 342 321 290
EcoO109I rG'GnC_Cy
Cuts at: 0 98 107 286 758 1016 1101 1620 1627
Size: 98 9 179 472 258 85 519 7
Cuts at: 1627 1723 1737
Size: 96 14
Fragments arranged by size:
519 472 258 179 98 96 85 14
9 7
HaeII r_GCGC'y
Cuts at: 0 298 355 1541 1737
Size: 298 57 1186 196
Fragments arranged by size:
1186 298 196 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 871 903 982 1014 1384 1416
Size: 106 32 733 32 79 32 370 32
Cuts at: 1416 1737
Size: 321
Fragments arranged by size:
733 370 321 106 79 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1131 1737
Size: 1131 606
KasI G'GCGC_C
Cuts at: 0 294 1737
Size: 294 1443
MslI CAynn'nnrTG
Cuts at: 0 790 1000 1737
Size: 790 210 737
Fragments arranged by size:
790 737 210
MspA1I CmG'CkG
Cuts at: 0 1146 1553 1737
Size: 1146 407 184
Fragments arranged by size:
1146 407 184
NaeI GCC'GGC
Cuts at: 0 80 447 1316 1737
Size: 80 367 869 421
Fragments arranged by size:
869 421 367 80
NarI GG'CG_CC
Cuts at: 0 295 1737
Size: 295 1442
NcoI C'CATG_G
Cuts at: 0 1281 1737
Size: 1281 456
NgoMIV G'CCGG_C
Cuts at: 0 78 445 1314 1737
Size: 78 367 869 423
Fragments arranged by size:
869 423 367 78
NheI G'CTAG_C
Cuts at: 0 1534 1737
Size: 1534 203
NruI TCG'CGA
Cuts at: 0 1199 1737
Size: 1199 538
NspI r_CATG'y
Cuts at: 0 414 1671 1737
Size: 414 1257 66
Fragments arranged by size:
1257 414 66
PasI CC'CwG_GG
Cuts at: 0 282 330 1737
Size: 282 48 1407
Fragments arranged by size:
1407 282 48
PciI A'CATG_T
Cuts at: 0 1667 1737
Size: 1667 70
PflMI CCAn_nnn'nTGG
Cuts at: 0 22 1737
Size: 22 1715
PfoI T'CCnGG_A
Cuts at: 0 142 506 1322 1451 1737
Size: 142 364 816 129 286
Fragments arranged by size:
816 364 286 142 129
PpuMI rG'GwC_Cy
Cuts at: 0 107 758 1101 1737
Size: 107 651 343 636
Fragments arranged by size:
651 636 343 107
PshAI GACnn'nnGTC
Cuts at: 0 342 1737
Size: 342 1395
PspOMI G'GGCC_C
Cuts at: 0 219 285 357 363 1626 1737
Size: 219 66 72 6 1263 111
Fragments arranged by size:
1263 219 111 72 66 6
PstI C_TGCA'G
Cuts at: 0 625 1145 1168 1737
Size: 625 520 23 569
Fragments arranged by size:
625 569 520 23
SacI G_AGCT'C
Cuts at: 0 506 962 1737
Size: 506 456 775
Fragments arranged by size:
775 506 456
SanDI GG'GwC_CC
Cuts at: 0 107 758 1737
Size: 107 651 979
Fragments arranged by size:
979 651 107
SexAI A'CCwGG_T
Cuts at: 0 377 1737
Size: 377 1360
SfcI C'TryA_G
Cuts at: 0 621 1141 1164 1737
Size: 621 520 23 573
Fragments arranged by size:
621 573 520 23
SfoI GGC'GCC
Cuts at: 0 296 1737
Size: 296 1441
SgrAI Cr'CCGG_yG
Cuts at: 0 946 1737
Size: 946 791
SmaI CCC'GGG
Cuts at: 0 224 362 1625 1632 1721 1737
Size: 224 138 1263 7 89 16
Fragments arranged by size:
1263 224 138 89 16 7
SpeI A'CTAG_T
Cuts at: 0 339 1737
Size: 339 1398
SphI G_CATG'C
Cuts at: 0 414 1737
Size: 414 1323
SrfI GCCC'GGGC
Cuts at: 0 224 362 1737
Size: 224 138 1375
Fragments arranged by size:
1375 224 138
StuI AGG'CCT
Cuts at: 0 814 1737
Size: 814 923
StyI C'CwwG_G
Cuts at: 0 316 456 1254 1281 1737
Size: 316 140 798 27 456
Fragments arranged by size:
798 456 316 140 27
TatI w'GTAC_w
Cuts at: 0 176 1737
Size: 176 1561
XmaI C'CCGG_G
Cuts at: 0 222 360 1623 1630 1719 1737
Size: 222 138 1263 7 89 18
Fragments arranged by size:
1263 222 138 89 18 7
XmnI GAAnn'nnTTC
Cuts at: 0 193 1737
Size: 193 1544
ZraI GAC'GTC
Cuts at: 0 1251 1737
Size: 1251 486
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AgeI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BcgI BglI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstEII BstXI BstYI Bsu36I BtgI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI
NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI
PstI SacI SanDI SexAI SfcI SfoI SgrAI SmaI
SpeI SphI SrfI StuI StyI TatI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AhdI AleI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI
BfrBI BglII BlpI BpuEI BsaBI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BssHII BstAPI BstBI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI MscI NdeI NotI NsiI
PacI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SfiI
SgfI SmlI SnaBI SspI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI