Restriction map for
pFN21AB7877
(Linear) (Six-base) MAPSORT of: KIBB7877.seq Check: 9698 from: 1 to: 1713
KIBB7877.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
Acc65I G'GTAC_C
Cuts at: 0 302 1713
Size: 302 1411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1199 1713
Size: 1123 76 514
Fragments arranged by size:
1123 514 76
AflIII A'CryG_T
Cuts at: 0 1393 1713
Size: 1393 320
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 958 990 1713
Size: 958 32 723
Fragments arranged by size:
958 723 32
AlwNI CAG_nnn'CTG
Cuts at: 0 75 181 1649 1713
Size: 75 106 1468 64
Fragments arranged by size:
1468 106 75 64
ApaI G_GGCC'C
Cuts at: 0 845 1175 1713
Size: 845 330 538
Fragments arranged by size:
845 538 330
ApoI r'AATT_y
Cuts at: 0 1114 1291 1713
Size: 1114 177 422
Fragments arranged by size:
1114 422 177
AvaI C'yCGr_G
Cuts at: 0 237 1713
Size: 237 1476
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 286 319 1713
Size: 286 33 1394
Fragments arranged by size:
1394 286 33
BamHI G'GATC_C
Cuts at: 0 1343 1713
Size: 1343 370
BanI G'GyrC_C
Cuts at: 0 219 302 367 1713
Size: 219 83 65 1346
Fragments arranged by size:
1346 219 83 65
BanII G_rGCy'C
Cuts at: 0 238 845 1085 1175 1713
Size: 238 607 240 90 538
Fragments arranged by size:
607 538 240 238 90
BbeI G_GCGC'C
Cuts at: 0 223 1713
Size: 223 1490
BbsI GAAGACnn'nnnn_
Cuts at: 0 149 737 886 1409 1712 1713
Size: 149 588 149 523 303 1
Fragments arranged by size:
588 523 303 149 149 1
BbvCI CC'TCA_GC
Cuts at: 0 490 1713
Size: 490 1223
BglI GCCn_nnn'nGGC
Cuts at: 0 1170 1713
Size: 1170 543
BglII A'GATC_T
Cuts at: 0 381 911 1713
Size: 381 530 802
Fragments arranged by size:
802 530 381
BlpI GC'TnA_GC
Cuts at: 0 232 1713
Size: 232 1481
Bme1580I G_kGCm'C
Cuts at: 0 845 993 1175 1713
Size: 845 148 182 538
Fragments arranged by size:
845 538 182 148
BmgBI CAC'GTC
Cuts at: 0 318 1713
Size: 318 1395
BmrI ACTGGGnnnn_n'
Cuts at: 0 495 1713
Size: 495 1218
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 278 1713
Size: 278 1435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 658 1472 1485 1713
Size: 187 471 814 13 228
Fragments arranged by size:
814 471 228 187 13
Bpu10I CC'TnA_GC
Cuts at: 0 343 490 1713
Size: 343 147 1223
Fragments arranged by size:
1223 343 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1240 1713
Size: 1240 473
BsaHI Gr'CG_yC
Cuts at: 0 220 1713
Size: 220 1493
BsaWI w'CCGG_w
Cuts at: 0 212 1713
Size: 212 1501
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 1713
Size: 610 1103
BseYI C'CCAG_C
Cuts at: 0 520 631 645 1713
Size: 520 111 14 1068
Fragments arranged by size:
1068 520 111 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 640 1045 1713
Size: 589 51 405 668
Fragments arranged by size:
668 589 405 51
BsiHKAI G_wGCw'C
Cuts at: 0 644 1085 1713
Size: 644 441 628
Fragments arranged by size:
644 628 441
Bsp1286I G_dGCh'C
Cuts at: 0 238 644 845 993 1085 1175 1713
Size: 238 406 201 148 92 90 538
Fragments arranged by size:
538 406 238 201 148 92 90
BspEI T'CCGG_A
Cuts at: 0 212 1713
Size: 212 1501
BspHI T'CATG_A
Cuts at: 0 1362 1701 1713
Size: 1362 339 12
Fragments arranged by size:
1362 339 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 611 1713
Size: 611 1102
BsrFI r'CCGG_y
Cuts at: 0 1334 1373 1713
Size: 1334 39 340
Fragments arranged by size:
1334 340 39
BssSI C'ACGA_G
Cuts at: 0 355 1713
Size: 355 1358
BstAPI GCAn_nnn'nTGC
Cuts at: 0 181 1585 1713
Size: 181 1404 128
Fragments arranged by size:
1404 181 128
BstXI CCAn_nnnn'nTGG
Cuts at: 0 400 1713
Size: 400 1313
BstYI r'GATC_y
Cuts at: 0 381 700 911 1343 1713
Size: 381 319 211 432 370
Fragments arranged by size:
432 381 370 319 211
Bsu36I CC'TnA_GG
Cuts at: 0 32 482 1713
Size: 32 450 1231
Fragments arranged by size:
1231 450 32
BtgI C'CryG_G
Cuts at: 0 134 298 1713
Size: 134 164 1415
Fragments arranged by size:
1415 164 134
BtsI GCAGTG_nn'
Cuts at: 0 381 1033 1713
Size: 381 652 680
Fragments arranged by size:
680 652 381
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 399 434 1545 1580 1713
Size: 399 35 1111 35 133
Fragments arranged by size:
1111 399 133 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1603 1713
Size: 1603 110
EaeI y'GGCC_r
Cuts at: 0 13 77 128 1713
Size: 13 64 51 1585
Fragments arranged by size:
1585 64 51 13
EarI CTCTTCn'nnn_
Cuts at: 0 716 1175 1713
Size: 716 459 538
Fragments arranged by size:
716 538 459
EcoICRI GAG'CTC
Cuts at: 0 1083 1713
Size: 1083 630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 658 1123 1199 1472 1485 1713
Size: 187 471 465 76 273 13 228
Fragments arranged by size:
471 465 273 228 187 76 13
EcoO109I rG'GnC_Cy
Cuts at: 0 494 600 842 1172 1713
Size: 494 106 242 330 541
Fragments arranged by size:
541 494 330 242 106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 754 1102 1713
Size: 754 348 611
Fragments arranged by size:
754 611 348
HaeII r_GCGC'y
Cuts at: 0 223 1713
Size: 223 1490
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 207 225 239 257 718 750 1621 1653
Size: 207 18 14 18 461 32 871 32
Cuts at: 1653 1713
Size: 60
Fragments arranged by size:
871 461 207 60 32 32 18 18
14
HincII GTy'rAC
Cuts at: 0 984 1713
Size: 984 729
KasI G'GCGC_C
Cuts at: 0 219 1713
Size: 219 1494
KpnI G_GTAC'C
Cuts at: 0 306 1713
Size: 306 1407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 578 1713
Size: 174 404 1135
Fragments arranged by size:
1135 404 174
MscI TGG'CCA
Cuts at: 0 130 1713
Size: 130 1583
MslI CAynn'nnrTG
Cuts at: 0 797 1361 1467 1713
Size: 797 564 106 246
Fragments arranged by size:
797 564 246 106
MspA1I CmG'CkG
Cuts at: 0 165 1713
Size: 165 1548
NarI GG'CG_CC
Cuts at: 0 220 1713
Size: 220 1493
NspI r_CATG'y
Cuts at: 0 1397 1440 1713
Size: 1397 43 273
Fragments arranged by size:
1397 273 43
PasI CC'CwG_GG
Cuts at: 0 323 1713
Size: 323 1390
PciI A'CATG_T
Cuts at: 0 1393 1713
Size: 1393 320
PflMI CCAn_nnn'nTGG
Cuts at: 0 796 1713
Size: 796 917
PpuMI rG'GwC_Cy
Cuts at: 0 494 600 1713
Size: 494 106 1113
Fragments arranged by size:
1113 494 106
PspOMI G'GGCC_C
Cuts at: 0 841 1171 1713
Size: 841 330 542
Fragments arranged by size:
841 542 330
PstI C_TGCA'G
Cuts at: 0 811 1191 1713
Size: 811 380 522
Fragments arranged by size:
811 522 380
PvuII CAG'CTG
Cuts at: 0 165 1713
Size: 165 1548
SacI G_AGCT'C
Cuts at: 0 1085 1713
Size: 1085 628
ScaI AGT'ACT
Cuts at: 0 1561 1713
Size: 1561 152
SfcI C'TryA_G
Cuts at: 0 114 250 807 1187 1197 1713
Size: 114 136 557 380 10 516
Fragments arranged by size:
557 516 380 136 114 10
SfoI GGC'GCC
Cuts at: 0 221 1713
Size: 221 1492
SmaI CCC'GGG
Cuts at: 0 239 1713
Size: 239 1474
SmlI C'TyrA_G
Cuts at: 0 1255 1713
Size: 1255 458
SspI AAT'ATT
Cuts at: 0 1237 1713
Size: 1237 476
StuI AGG'CCT
Cuts at: 0 674 1713
Size: 674 1039
StyI C'CwwG_G
Cuts at: 0 1001 1713
Size: 1001 712
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 534 659 1713
Size: 534 125 1054
Fragments arranged by size:
1054 534 125
TatI w'GTAC_w
Cuts at: 0 1259 1480 1559 1713
Size: 1259 221 79 154
Fragments arranged by size:
1259 221 154 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 60 432 464 1713
Size: 28 32 372 32 1249
Fragments arranged by size:
1249 372 32 32 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 397 400 943 1713
Size: 397 3 543 770
Fragments arranged by size:
770 543 397 3
XmaI C'CCGG_G
Cuts at: 0 237 1713
Size: 237 1476
XmnI GAAnn'nnTTC
Cuts at: 0 1405 1546 1660 1713
Size: 1405 141 114 53
Fragments arranged by size:
1405 141 114 53
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApaI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtsI CspCI DrdI EaeI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NarI NspI PasI PciI
PflMI PpuMI PspOMI PstI PvuII SacI ScaI SfcI
SfoI SmaI SmlI SspI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BcgI
BciVI BclI BfrBI BmtI BsaI BsaAI BsaBI BsaXI
BsiEI BsiWI BsmI BsmBI BsrBI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII EagI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XhoI ZraI