Restriction map for
pFN21AB7870
(Linear) (Six-base) MAPSORT of: KIBB7870.seq Check: 5563 from: 1 to: 1764
KIBB7870.orf
With 182 enzymes: SgfI *
March 1, 2012 17:47 ..
AccI GT'mk_AC
Cuts at: 0 1372 1764
Size: 1372 392
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1725 1764
Size: 370 1355 39
Fragments arranged by size:
1355 370 39
AflII C'TTAA_G
Cuts at: 0 415 1764
Size: 415 1349
AflIII A'CryG_T
Cuts at: 0 670 672 1764
Size: 670 2 1092
Fragments arranged by size:
1092 670 2
ApaLI G'TGCA_C
Cuts at: 0 1637 1764
Size: 1637 127
ApoI r'AATT_y
Cuts at: 0 348 464 784 964 994 1162 1424 1764
Size: 348 116 320 180 30 168 262 340
Fragments arranged by size:
348 340 320 262 180 168 116 30
AseI AT'TA_AT
Cuts at: 0 947 1764
Size: 947 817
BanII G_rGCy'C
Cuts at: 0 401 1764
Size: 401 1363
BbsI GAAGACnn'nnnn_
Cuts at: 0 1442 1764
Size: 1442 322
BclI T'GATC_A
Cuts at: 0 507 1764
Size: 507 1257
BglII A'GATC_T
Cuts at: 0 328 1764
Size: 328 1436
Bme1580I G_kGCm'C
Cuts at: 0 1641 1764
Size: 1641 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1764
Size: 1310 454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 672 834 1764
Size: 345 327 162 930
Fragments arranged by size:
930 345 327 162
BsaI GGTCTCn'nnnn_
Cuts at: 0 1721 1764
Size: 1721 43
BsaAI yAC'GTr
Cuts at: 0 673 1764
Size: 673 1091
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 20 40 50 70 1764
Size: 20 20 10 20 1694
Fragments arranged by size:
1694 20 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 61 225 1615 1764
Size: 28 33 164 1390 149
Fragments arranged by size:
1390 164 149 33 28
BsiHKAI G_wGCw'C
Cuts at: 0 401 779 1641 1764
Size: 401 378 862 123
Fragments arranged by size:
862 401 378 123
Bsp1286I G_dGCh'C
Cuts at: 0 401 779 1641 1764
Size: 401 378 862 123
Fragments arranged by size:
862 401 378 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 895 1764
Size: 895 869
BsrDI GCAATG_nn'
Cuts at: 0 59 257 798 1764
Size: 59 198 541 966
Fragments arranged by size:
966 541 198 59
BstXI CCAn_nnnn'nTGG
Cuts at: 0 805 1764
Size: 805 959
BstYI r'GATC_y
Cuts at: 0 328 1764
Size: 328 1436
Bsu36I CC'TnA_GG
Cuts at: 0 1043 1764
Size: 1043 721
BtgI C'CryG_G
Cuts at: 0 272 1682 1764
Size: 272 1410 82
Fragments arranged by size:
1410 272 82
BtsI GCAGTG_nn'
Cuts at: 0 989 1764
Size: 989 775
DraI TTT'AAA
Cuts at: 0 335 1196 1764
Size: 335 861 568
Fragments arranged by size:
861 568 335
EaeI y'GGCC_r
Cuts at: 0 896 1764
Size: 896 868
EarI CTCTTCn'nnn_
Cuts at: 0 5 475 734 1764
Size: 5 470 259 1030
Fragments arranged by size:
1030 470 259 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1764
Size: 22 1742
EcoICRI GAG'CTC
Cuts at: 0 399 1764
Size: 399 1365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1310 1725 1764
Size: 370 940 415 39
Fragments arranged by size:
940 415 370 39
EcoO109I rG'GnC_Cy
Cuts at: 0 1719 1764
Size: 1719 45
EcoRV GAT'ATC
Cuts at: 0 1036 1764
Size: 1036 728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 713 1764
Size: 713 1051
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 395 427 1764
Size: 395 32 1337
Fragments arranged by size:
1337 395 32
HincII GTy'rAC
Cuts at: 0 1260 1764
Size: 1260 504
HindIII A'AGCT_T
Cuts at: 0 531 1764
Size: 531 1233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 688 819 1501 1625 1764
Size: 426 262 131 682 124 139
Fragments arranged by size:
682 426 262 139 131 124
MscI TGG'CCA
Cuts at: 0 898 1764
Size: 898 866
MslI CAynn'nnrTG
Cuts at: 0 1085 1613 1681 1764
Size: 1085 528 68 83
Fragments arranged by size:
1085 528 83 68
NcoI C'CATG_G
Cuts at: 0 272 1682 1764
Size: 272 1410 82
Fragments arranged by size:
1410 272 82
PasI CC'CwG_GG
Cuts at: 0 1732 1764
Size: 1732 32
PmlI CAC'GTG
Cuts at: 0 673 1764
Size: 673 1091
PsiI TTA'TAA
Cuts at: 0 970 1764
Size: 970 794
PstI C_TGCA'G
Cuts at: 0 426 1516 1764
Size: 426 1090 248
Fragments arranged by size:
1090 426 248
SacI G_AGCT'C
Cuts at: 0 401 1764
Size: 401 1363
SapI GCTCTTCn'nnn_
Cuts at: 0 734 1764
Size: 734 1030
ScaI AGT'ACT
Cuts at: 0 1385 1596 1764
Size: 1385 211 168
Fragments arranged by size:
1385 211 168
SfcI C'TryA_G
Cuts at: 0 422 1126 1512 1764
Size: 422 704 386 252
Fragments arranged by size:
704 422 386 252
SmlI C'TyrA_G
Cuts at: 0 324 415 687 813 1764
Size: 324 91 272 126 951
Fragments arranged by size:
951 324 272 126 91
SspI AAT'ATT
Cuts at: 0 834 1075 1764
Size: 834 241 689
Fragments arranged by size:
834 689 241
StyI C'CwwG_G
Cuts at: 0 272 1225 1682 1764
Size: 272 953 457 82
Fragments arranged by size:
953 457 272 82
TatI w'GTAC_w
Cuts at: 0 1383 1594 1764
Size: 1383 211 170
Fragments arranged by size:
1383 211 170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 20 40 52 72 1764
Size: 20 20 12 20 1692
Fragments arranged by size:
1692 20 20 20 12
XbaI T'CTAG_A
Cuts at: 0 1339 1764
Size: 1339 425
XmnI GAAnn'nnTTC
Cuts at: 0 165 1076 1764
Size: 165 911 688
Fragments arranged by size:
911 688 165
Enzymes that do cut:
AccI AcuI AflII AflIII ApaLI ApoI AseI BanII
BbsI BclI BglII Bme1580I BpmI BpuEI BsaI BsaAI
BsaXI BseRI BsiHKAI Bsp1286I BspMI BsrDI BstXI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII MmeI
MscI MslI NcoI PasI PmlI PsiI PstI SacI
SapI ScaI SfcI SmlI SspI StyI TatI TstI
XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EcoNI EcoRI FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XcmI XhoI
XmaI ZraI