Restriction map for
pFN21AB7862
(Linear) (Six-base) MAPSORT of: KIBB7862.seq Check: 9637 from: 1 to: 1890
KIBB7862.orf
With 182 enzymes: SgfI *
July 3, 2012 16:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 1890
Size: 1140 750
AflIII A'CryG_T
Cuts at: 0 498 1182 1266 1854 1890
Size: 498 684 84 588 36
Fragments arranged by size:
684 588 498 84 36
AleI CACnn'nnGTG
Cuts at: 0 1199 1890
Size: 1199 691
AlwNI CAG_nnn'CTG
Cuts at: 0 27 458 1890
Size: 27 431 1432
Fragments arranged by size:
1432 431 27
ApoI r'AATT_y
Cuts at: 0 526 545 558 791 1106 1358 1890
Size: 526 19 13 233 315 252 532
Fragments arranged by size:
532 526 315 252 233 19 13
AvaI C'yCGr_G
Cuts at: 0 115 1890
Size: 115 1775
BamHI G'GATC_C
Cuts at: 0 147 1890
Size: 147 1743
BanII G_rGCy'C
Cuts at: 0 116 1890
Size: 116 1774
BclI T'GATC_A
Cuts at: 0 573 1890
Size: 573 1317
BfrBI ATG'CAT
Cuts at: 0 1840 1890
Size: 1840 50
BglII A'GATC_T
Cuts at: 0 1189 1890
Size: 1189 701
BmrI ACTGGGnnnn_n'
Cuts at: 0 287 478 956 1712 1890
Size: 287 191 478 756 178
Fragments arranged by size:
756 478 287 191 178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 205 821 1136 1640 1808 1890
Size: 115 90 616 315 504 168 82
Fragments arranged by size:
616 504 315 168 115 90 82
Bpu10I CC'TnA_GC
Cuts at: 0 829 1890
Size: 829 1061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 564 842 1890
Size: 419 145 278 1048
Fragments arranged by size:
1048 419 278 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 12 34 1890
Size: 12 22 1856
Fragments arranged by size:
1856 22 12
BsaWI w'CCGG_w
Cuts at: 0 50 1030 1890
Size: 50 980 860
Fragments arranged by size:
980 860 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 45 75 226 256 1890
Size: 45 30 151 30 1634
Fragments arranged by size:
1634 151 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 394 1890
Size: 69 325 1496
Fragments arranged by size:
1496 325 69
BseYI C'CCAG_C
Cuts at: 0 823 1890
Size: 823 1067
BsmI GAATG_Cn'
Cuts at: 0 1306 1890
Size: 1306 584
Bsp1286I G_dGCh'C
Cuts at: 0 116 1890
Size: 116 1774
BspHI T'CATG_A
Cuts at: 0 1884 1890
Size: 1884 6
BsrDI GCAATG_nn'
Cuts at: 0 1311 1890
Size: 1311 579
BsrGI T'GTAC_A
Cuts at: 0 1011 1683 1890
Size: 1011 672 207
Fragments arranged by size:
1011 672 207
BstYI r'GATC_y
Cuts at: 0 147 1189 1890
Size: 147 1042 701
Fragments arranged by size:
1042 701 147
Bsu36I CC'TnA_GG
Cuts at: 0 406 1890
Size: 406 1484
BtgI C'CryG_G
Cuts at: 0 44 128 268 1071 1890
Size: 44 84 140 803 819
Fragments arranged by size:
819 803 140 84 44
BtsI GCAGTG_nn'
Cuts at: 0 459 1890
Size: 459 1431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1293 1328 1890
Size: 1293 35 562
Fragments arranged by size:
1293 562 35
DraI TTT'AAA
Cuts at: 0 1578 1890
Size: 1578 312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 205 821 1136 1140 1640 1808 1890
Size: 115 90 616 315 4 500 168 82
Fragments arranged by size:
616 500 315 168 115 90 82 4
EcoRI G'AATT_C
Cuts at: 0 1106 1358 1890
Size: 1106 252 532
Fragments arranged by size:
1106 532 252
HaeII r_GCGC'y
Cuts at: 0 128 1890
Size: 128 1762
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 682 714 1890
Size: 226 32 424 32 1176
Fragments arranged by size:
1176 424 226 32 32
HindIII A'AGCT_T
Cuts at: 0 860 1403 1890
Size: 860 543 487
Fragments arranged by size:
860 543 487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 1890
Size: 230 1660
MslI CAynn'nnrTG
Cuts at: 0 963 1047 1082 1199 1215 1283 1299 1367
Size: 963 84 35 117 16 68 16 68
Cuts at: 1367 1535 1890
Size: 168 355
Fragments arranged by size:
963 355 168 117 84 68 68 35
16 16
MspA1I CmG'CkG
Cuts at: 0 14 130 1890
Size: 14 116 1760
Fragments arranged by size:
1760 116 14
NcoI C'CATG_G
Cuts at: 0 268 1071 1890
Size: 268 803 819
Fragments arranged by size:
819 803 268
NdeI CA'TA_TG
Cuts at: 0 960 1044 1212 1296 1520 1890
Size: 960 84 168 84 224 370
Fragments arranged by size:
960 370 224 168 84 84
NsiI A_TGCA'T
Cuts at: 0 1842 1890
Size: 1842 48
NspI r_CATG'y
Cuts at: 0 502 1087 1186 1270 1858 1890
Size: 502 585 99 84 588 32
Fragments arranged by size:
588 585 502 99 84 32
PciI A'CATG_T
Cuts at: 0 498 1182 1266 1854 1890
Size: 498 684 84 588 36
Fragments arranged by size:
684 588 498 84 36
PsiI TTA'TAA
Cuts at: 0 1466 1890
Size: 1466 424
SacII CC_GC'GG
Cuts at: 0 131 1890
Size: 131 1759
SmlI C'TyrA_G
Cuts at: 0 434 579 857 1890
Size: 434 145 278 1033
Fragments arranged by size:
1033 434 278 145
StuI AGG'CCT
Cuts at: 0 1592 1890
Size: 1592 298
StyI C'CwwG_G
Cuts at: 0 268 1071 1890
Size: 268 803 819
Fragments arranged by size:
819 803 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 837 1890
Size: 837 1053
TatI w'GTAC_w
Cuts at: 0 1011 1683 1890
Size: 1011 672 207
Fragments arranged by size:
1011 672 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 45 77 1890
Size: 45 32 1813
Fragments arranged by size:
1813 45 32
Tth111I GACn'n_nGTC
Cuts at: 0 293 1890
Size: 293 1597
XmnI GAAnn'nnTTC
Cuts at: 0 631 1052 1153 1405 1825 1890
Size: 631 421 101 252 420 65
Fragments arranged by size:
631 421 420 252 101 65
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI AvaI BamHI BanII
BclI BfrBI BglII BmrI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsmI Bsp1286I BspHI BsrDI
BsrGI BstYI Bsu36I BtgI BtsI CspCI DraI Eco57MI
EcoRI HaeII Hin4I HindIII MmeI MslI MspA1I NcoI
NdeI NsiI NspI PciI PsiI SacII SmlI StuI
StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmtI BplI BsaAI BsaBI
BsaHI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI ClaI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XcmI XhoI XmaI ZraI