Restriction map for
pFN21AB7848
(Linear) (Six-base) MAPSORT of: KIBB7848.seq Check: 7464 from: 1 to: 1866
KIBB7848.orf
With 182 enzymes: SgfI *
July 3, 2012 16:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 642 1866
Size: 642 1224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1866
Size: 1129 737
AflIII A'CryG_T
Cuts at: 0 116 1866
Size: 116 1750
AhdI GACnn_n'nnGTC
Cuts at: 0 1069 1866
Size: 1069 797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1585 1866
Size: 1585 281
AlwNI CAG_nnn'CTG
Cuts at: 0 898 1791 1866
Size: 898 893 75
Fragments arranged by size:
898 893 75
ApaI G_GGCC'C
Cuts at: 0 213 698 1547 1615 1866
Size: 213 485 849 68 251
Fragments arranged by size:
849 485 251 213 68
AvaI C'yCGr_G
Cuts at: 0 38 1405 1505 1615 1866
Size: 38 1367 100 110 251
Fragments arranged by size:
1367 251 110 100 38
BanI G'GyrC_C
Cuts at: 0 420 435 1163 1169 1369 1596 1624 1666
Size: 420 15 728 6 200 227 28 42
Cuts at: 1666 1724 1866
Size: 58 142
Fragments arranged by size:
728 420 227 200 142 58 42 28
15 6
BanII G_rGCy'C
Cuts at: 0 213 698 1136 1331 1504 1547 1615 1866
Size: 213 485 438 195 173 43 68 251
Fragments arranged by size:
485 438 251 213 195 173 68 43
BbeI G_GCGC'C
Cuts at: 0 1173 1670 1728 1866
Size: 1173 497 58 138
Fragments arranged by size:
1173 497 138 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 443 1866
Size: 443 1423
BglI GCCn_nnn'nGGC
Cuts at: 0 1154 1490 1508 1758 1866
Size: 1154 336 18 250 108
Fragments arranged by size:
1154 336 250 108 18
BlpI GC'TnA_GC
Cuts at: 0 82 811 1866
Size: 82 729 1055
Fragments arranged by size:
1055 729 82
Bme1580I G_kGCm'C
Cuts at: 0 213 440 573 698 1547 1599 1615 1866
Size: 213 227 133 125 849 52 16 251
Fragments arranged by size:
849 251 227 213 133 125 52 16
BmgBI CAC'GTC
Cuts at: 0 117 1866
Size: 117 1749
BmrI ACTGGGnnnn_n'
Cuts at: 0 434 1866
Size: 434 1432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1233 1866
Size: 1233 633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 523 649 760 1866
Size: 97 426 126 111 1106
Fragments arranged by size:
1106 426 126 111 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 457 1866
Size: 410 47 1409
Fragments arranged by size:
1409 410 47
BsaAI yAC'GTr
Cuts at: 0 1782 1866
Size: 1782 84
BsaHI Gr'CG_yC
Cuts at: 0 1170 1667 1725 1866
Size: 1170 497 58 141
Fragments arranged by size:
1170 497 141 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 917 947 1866
Size: 223 30 664 30 919
Fragments arranged by size:
919 664 223 30 30
BseYI C'CCAG_C
Cuts at: 0 1237 1705 1866
Size: 1237 468 161
Fragments arranged by size:
1237 468 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1250 1367 1804 1866
Size: 622 628 117 437 62
Fragments arranged by size:
628 622 437 117 62
BsiEI CG_ry'CG
Cuts at: 0 907 1402 1806 1866
Size: 907 495 404 60
Fragments arranged by size:
907 495 404 60
BsiHKAI G_wGCw'C
Cuts at: 0 70 1331 1866
Size: 70 1261 535
Fragments arranged by size:
1261 535 70
BsiWI C'GTAC_G
Cuts at: 0 1754 1866
Size: 1754 112
Bsp1286I G_dGCh'C
Cuts at: 0 70 213 440 573 698 1136 1331 1504
Size: 70 143 227 133 125 438 195 173
Cuts at: 1504 1547 1599 1615 1866
Size: 43 52 16 251
Fragments arranged by size:
438 251 227 195 173 143 133 125
70 52 43 16
BspHI T'CATG_A
Cuts at: 0 161 1866
Size: 161 1705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 642 1190 1866
Size: 642 548 676
Fragments arranged by size:
676 642 548
BsrBI CCG'CTC
Cuts at: 0 18 909 1863 1866
Size: 18 891 954 3
Fragments arranged by size:
954 891 18 3
BsrFI r'CCGG_y
Cuts at: 0 641 1166 1372 1422 1621 1826 1866
Size: 641 525 206 50 199 205 40
Fragments arranged by size:
641 525 206 205 199 50 40
BssHII G'CGCG_C
Cuts at: 0 1588 1866
Size: 1588 278
BssSI C'ACGA_G
Cuts at: 0 494 1866
Size: 494 1372
BstAPI GCAn_nnn'nTGC
Cuts at: 0 645 867 1017 1163 1791 1866
Size: 645 222 150 146 628 75
Fragments arranged by size:
645 628 222 150 146 75
BstEII G'GTnAC_C
Cuts at: 0 1302 1866
Size: 1302 564
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1089 1866
Size: 1089 777
BstYI r'GATC_y
Cuts at: 0 32 884 894 1866
Size: 32 852 10 972
Fragments arranged by size:
972 852 32 10
Bsu36I CC'TnA_GG
Cuts at: 0 305 1866
Size: 305 1561
BtgI C'CryG_G
Cuts at: 0 929 1186 1388 1396 1719 1866
Size: 929 257 202 8 323 147
Fragments arranged by size:
929 323 257 202 147 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 101 1866
Size: 101 1765
BtsI GCAGTG_nn'
Cuts at: 0 262 726 1866
Size: 262 464 1140
Fragments arranged by size:
1140 464 262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1125 1160 1347 1382 1866
Size: 1125 35 187 35 484
Fragments arranged by size:
1125 484 187 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 489 1866
Size: 489 1377
EaeI y'GGCC_r
Cuts at: 0 904 1310 1355 1374 1399 1803 1866
Size: 904 406 45 19 25 404 63
Fragments arranged by size:
904 406 404 63 45 25 19
EagI C'GGCC_G
Cuts at: 0 904 1399 1803 1866
Size: 904 495 404 63
Fragments arranged by size:
904 495 404 63
EarI CTCTTCn'nnn_
Cuts at: 0 8 1639 1866
Size: 8 1631 227
Fragments arranged by size:
1631 227 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 1532 1866
Size: 76 1456 334
Fragments arranged by size:
1456 334 76
EcoICRI GAG'CTC
Cuts at: 0 1329 1866
Size: 1329 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 523 649 760 1129 1866
Size: 97 426 126 111 369 737
Fragments arranged by size:
737 426 369 126 111 97
EcoO109I rG'GnC_Cy
Cuts at: 0 209 210 694 695 837 1287 1565 1611
Size: 209 1 484 1 142 450 278 46
Cuts at: 1611 1612 1866
Size: 1 254
Fragments arranged by size:
484 450 278 254 209 142 46 1
1 1
HaeII r_GCGC'y
Cuts at: 0 103 1173 1475 1670 1716 1728 1837 1866
Size: 103 1070 302 195 46 12 109 29
Fragments arranged by size:
1070 302 195 109 103 46 29 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 374 406 1055 1087 1866
Size: 374 32 649 32 779
Fragments arranged by size:
779 649 374 32 32
KasI G'GCGC_C
Cuts at: 0 1169 1666 1724 1866
Size: 1169 497 58 142
Fragments arranged by size:
1169 497 142 58
MscI TGG'CCA
Cuts at: 0 1357 1866
Size: 1357 509
MslI CAynn'nnrTG
Cuts at: 0 1087 1866
Size: 1087 779
MspA1I CmG'CkG
Cuts at: 0 376 707 1260 1398 1461 1514 1721 1866
Size: 376 331 553 138 63 53 207 145
Fragments arranged by size:
553 376 331 207 145 138 63 53
NaeI GCC'GGC
Cuts at: 0 1168 1424 1866
Size: 1168 256 442
Fragments arranged by size:
1168 442 256
NarI GG'CG_CC
Cuts at: 0 1170 1667 1725 1866
Size: 1170 497 58 141
Fragments arranged by size:
1170 497 141 58
NcoI C'CATG_G
Cuts at: 0 1186 1866
Size: 1186 680
NgoMIV G'CCGG_C
Cuts at: 0 1166 1422 1866
Size: 1166 256 444
Fragments arranged by size:
1166 444 256
NotI GC'GGCC_GC
Cuts at: 0 904 1399 1866
Size: 904 495 467
Fragments arranged by size:
904 495 467
NspI r_CATG'y
Cuts at: 0 946 1866
Size: 946 920
PflMI CCAn_nnn'nTGG
Cuts at: 0 627 998 1866
Size: 627 371 868
Fragments arranged by size:
868 627 371
PfoI T'CCnGG_A
Cuts at: 0 539 737 1866
Size: 539 198 1129
Fragments arranged by size:
1129 539 198
PpuMI rG'GwC_Cy
Cuts at: 0 837 1287 1866
Size: 837 450 579
Fragments arranged by size:
837 579 450
PshAI GACnn'nnGTC
Cuts at: 0 376 1866
Size: 376 1490
PspOMI G'GGCC_C
Cuts at: 0 209 694 1543 1611 1866
Size: 209 485 849 68 255
Fragments arranged by size:
849 485 255 209 68
PstI C_TGCA'G
Cuts at: 0 653 881 1866
Size: 653 228 985
Fragments arranged by size:
985 653 228
PvuII CAG'CTG
Cuts at: 0 376 1461 1866
Size: 376 1085 405
Fragments arranged by size:
1085 405 376
SacI G_AGCT'C
Cuts at: 0 1331 1866
Size: 1331 535
SacII CC_GC'GG
Cuts at: 0 1399 1722 1866
Size: 1399 323 144
Fragments arranged by size:
1399 323 144
SapI GCTCTTCn'nnn_
Cuts at: 0 8 1639 1866
Size: 8 1631 227
Fragments arranged by size:
1631 227 8
ScaI AGT'ACT
Cuts at: 0 478 1866
Size: 478 1388
SexAI A'CCwGG_T
Cuts at: 0 323 485 1866
Size: 323 162 1381
Fragments arranged by size:
1381 323 162
SfcI C'TryA_G
Cuts at: 0 649 877 1866
Size: 649 228 989
Fragments arranged by size:
989 649 228
SfoI GGC'GCC
Cuts at: 0 1171 1668 1726 1866
Size: 1171 497 58 140
Fragments arranged by size:
1171 497 140 58
SgrAI Cr'CCGG_yG
Cuts at: 0 1826 1866
Size: 1826 40
SmaI CCC'GGG
Cuts at: 0 1407 1507 1617 1866
Size: 1407 100 110 249
Fragments arranged by size:
1407 249 110 100
SmlI C'TyrA_G
Cuts at: 0 425 472 1866
Size: 425 47 1394
Fragments arranged by size:
1394 425 47
SphI G_CATG'C
Cuts at: 0 946 1866
Size: 946 920
StyI C'CwwG_G
Cuts at: 0 1186 1866
Size: 1186 680
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1401 1866
Size: 1401 465
TatI w'GTAC_w
Cuts at: 0 476 1853 1866
Size: 476 1377 13
Fragments arranged by size:
1377 476 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 917 949 1866
Size: 223 32 662 32 917
Fragments arranged by size:
917 662 223 32 32
XmaI C'CCGG_G
Cuts at: 0 1405 1505 1615 1866
Size: 1405 100 110 251
Fragments arranged by size:
1405 251 110 100
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaAI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SapI ScaI SexAI SfcI SfoI SgrAI SmaI
SmlI SphI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmtI
Bpu10I BsaI BsaBI BsaWI BseRI BsmI BsmBI BspEI
BsrDI BsrGI BstBI BstZ17I ClaI DraI DrdI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NdeI NheI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI SfiI
SgfI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmnI ZraI