Restriction map for
pFN21AB7847
(Linear) (Six-base) MAPSORT of: KIBB7847.seq Check: 6151 from: 1 to: 1635
KIBB7847.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1137 1221 1635
Size: 1137 84 414
Fragments arranged by size:
1137 414 84
AfeI AGC'GCT
Cuts at: 0 163 1635
Size: 163 1472
AflIII A'CryG_T
Cuts at: 0 1555 1635
Size: 1555 80
AhdI GACnn_n'nnGTC
Cuts at: 0 39 1635
Size: 39 1596
AleI CACnn'nnGTG
Cuts at: 0 1028 1112 1529 1635
Size: 1028 84 417 106
Fragments arranged by size:
1028 417 106 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 953 1635
Size: 823 130 682
Fragments arranged by size:
823 682 130
AlwNI CAG_nnn'CTG
Cuts at: 0 276 303 898 1635
Size: 276 27 595 737
Fragments arranged by size:
737 595 276 27
ApaI G_GGCC'C
Cuts at: 0 145 199 1635
Size: 145 54 1436
Fragments arranged by size:
1436 145 54
ApoI r'AATT_y
Cuts at: 0 1271 1329 1635
Size: 1271 58 306
Fragments arranged by size:
1271 306 58
AvaI C'yCGr_G
Cuts at: 0 137 1635
Size: 137 1498
BamHI G'GATC_C
Cuts at: 0 547 1635
Size: 547 1088
BanI G'GyrC_C
Cuts at: 0 414 1635
Size: 414 1221
BanII G_rGCy'C
Cuts at: 0 65 145 199 1081 1635
Size: 65 80 54 882 554
Fragments arranged by size:
882 554 80 65 54
BbeI G_GCGC'C
Cuts at: 0 418 1635
Size: 418 1217
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 872 1058 1143 1237 1635
Size: 56 816 186 85 94 398
Fragments arranged by size:
816 398 186 94 85 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 212 246 944 978 1635
Size: 212 34 698 34 657
Fragments arranged by size:
698 657 212 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 106 1635
Size: 106 1529
Bme1580I G_kGCm'C
Cuts at: 0 145 199 1635
Size: 145 54 1436
Fragments arranged by size:
1436 145 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 33 1205 1289 1635
Size: 33 1172 84 346
Fragments arranged by size:
1172 346 84 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 111 1004 1635
Size: 111 893 631
Fragments arranged by size:
893 631 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 313 559 626 797 1635
Size: 298 15 246 67 171 838
Fragments arranged by size:
838 298 246 171 67 15
Bpu10I CC'TnA_GC
Cuts at: 0 1385 1635
Size: 1385 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1635
Size: 470 1165
BsaI GGTCTCn'nnnn_
Cuts at: 0 497 774 1635
Size: 497 277 861
Fragments arranged by size:
861 497 277
BsaHI Gr'CG_yC
Cuts at: 0 35 415 1635
Size: 35 380 1220
Fragments arranged by size:
1220 380 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 675 705 901 931 1431 1461 1635
Size: 675 30 196 30 500 30 174
Fragments arranged by size:
675 500 196 174 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 1635
Size: 379 1256
BseYI C'CCAG_C
Cuts at: 0 332 551 1635
Size: 332 219 1084
Fragments arranged by size:
1084 332 219
BsiHKAI G_wGCw'C
Cuts at: 0 123 1081 1391 1635
Size: 123 958 310 244
Fragments arranged by size:
958 310 244 123
Bsp1286I G_dGCh'C
Cuts at: 0 65 123 145 199 1081 1391 1635
Size: 65 58 22 54 882 310 244
Fragments arranged by size:
882 310 244 65 58 54 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 411 905 1635
Size: 411 494 730
Fragments arranged by size:
730 494 411
BsrBI CCG'CTC
Cuts at: 0 359 1635
Size: 359 1276
BsrDI GCAATG_nn'
Cuts at: 0 626 869 1635
Size: 626 243 766
Fragments arranged by size:
766 626 243
BsrFI r'CCGG_y
Cuts at: 0 215 411 1635
Size: 215 196 1224
Fragments arranged by size:
1224 215 196
BstAPI GCAn_nnn'nTGC
Cuts at: 0 450 1635
Size: 450 1185
BstEII G'GTnAC_C
Cuts at: 0 433 1635
Size: 433 1202
BstXI CCAn_nnnn'nTGG
Cuts at: 0 583 973 1635
Size: 583 390 662
Fragments arranged by size:
662 583 390
BstYI r'GATC_y
Cuts at: 0 272 547 1635
Size: 272 275 1088
Fragments arranged by size:
1088 275 272
Bsu36I CC'TnA_GG
Cuts at: 0 859 1635
Size: 859 776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1290 1325 1635
Size: 1290 35 310
Fragments arranged by size:
1290 310 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 87 124 1341 1635
Size: 87 37 1217 294
Fragments arranged by size:
1217 294 87 37
EcoICRI GAG'CTC
Cuts at: 0 1079 1635
Size: 1079 556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 313 559 626 797 1137 1221 1635
Size: 298 15 246 67 171 340 84 414
Fragments arranged by size:
414 340 298 246 171 84 67 15
EcoO109I rG'GnC_Cy
Cuts at: 0 141 142 196 647 1635
Size: 141 1 54 451 988
Fragments arranged by size:
988 451 141 54 1
EcoRI G'AATT_C
Cuts at: 0 1329 1635
Size: 1329 306
HaeII r_GCGC'y
Cuts at: 0 165 418 1635
Size: 165 253 1217
Fragments arranged by size:
1217 253 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 468 500 675 707 1635
Size: 25 32 411 32 175 32 928
Fragments arranged by size:
928 411 175 32 32 32 25
HindIII A'AGCT_T
Cuts at: 0 823 1635
Size: 823 812
KasI G'GCGC_C
Cuts at: 0 414 1635
Size: 414 1221
MslI CAynn'nnrTG
Cuts at: 0 971 1028 1112 1128 1296 1529 1635
Size: 971 57 84 16 168 233 106
Fragments arranged by size:
971 233 168 106 84 57 16
MspA1I CmG'CkG
Cuts at: 0 402 708 1635
Size: 402 306 927
Fragments arranged by size:
927 402 306
NaeI GCC'GGC
Cuts at: 0 217 413 1635
Size: 217 196 1222
Fragments arranged by size:
1222 217 196
NarI GG'CG_CC
Cuts at: 0 415 1635
Size: 415 1220
NdeI CA'TA_TG
Cuts at: 0 1125 1635
Size: 1125 510
NgoMIV G'CCGG_C
Cuts at: 0 215 411 1635
Size: 215 196 1224
Fragments arranged by size:
1224 215 196
NspI r_CATG'y
Cuts at: 0 892 1559 1635
Size: 892 667 76
Fragments arranged by size:
892 667 76
PasI CC'CwG_GG
Cuts at: 0 594 651 1635
Size: 594 57 984
Fragments arranged by size:
984 594 57
PciI A'CATG_T
Cuts at: 0 1555 1635
Size: 1555 80
PfoI T'CCnGG_A
Cuts at: 0 275 1635
Size: 275 1360
PpuMI rG'GwC_Cy
Cuts at: 0 647 1635
Size: 647 988
PsiI TTA'TAA
Cuts at: 0 1460 1635
Size: 1460 175
PspOMI G'GGCC_C
Cuts at: 0 141 195 1635
Size: 141 54 1440
Fragments arranged by size:
1440 141 54
PstI C_TGCA'G
Cuts at: 0 48 130 248 329 610 1252 1635
Size: 48 82 118 81 281 642 383
Fragments arranged by size:
642 383 281 118 82 81 48
PvuII CAG'CTG
Cuts at: 0 402 708 1635
Size: 402 306 927
Fragments arranged by size:
927 402 306
SacI G_AGCT'C
Cuts at: 0 1081 1635
Size: 1081 554
SanDI GG'GwC_CC
Cuts at: 0 647 1635
Size: 647 988
SfcI C'TryA_G
Cuts at: 0 44 126 244 325 606 732 1248 1635
Size: 44 82 118 81 281 126 516 387
Fragments arranged by size:
516 387 281 126 118 82 81 44
SfoI GGC'GCC
Cuts at: 0 416 1635
Size: 416 1219
SmlI C'TyrA_G
Cuts at: 0 485 1635
Size: 485 1150
StuI AGG'CCT
Cuts at: 0 1001 1635
Size: 1001 634
StyI C'CwwG_G
Cuts at: 0 774 1635
Size: 774 861
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 324 686 1635
Size: 324 362 949
Fragments arranged by size:
949 362 324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1347 1379 1381 1413 1414 1446 1635
Size: 1347 32 2 32 1 32 189
Fragments arranged by size:
1347 189 32 32 32 2 1
XmnI GAAnn'nnTTC
Cuts at: 0 1234 1635
Size: 1234 401
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I CspCI
EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII
KasI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PasI PciI PfoI PpuMI PsiI PspOMI PstI PvuII
SacI SanDI SfcI SfoI SmlI StuI StyI TaqII
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI
BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstBI BstZ17I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EcoNI
EcoRV FalI FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MmeI MscI NcoI NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI ZraI