Restriction map for
pFN21AB7846
(Linear) (Six-base) MAPSORT of: KIBB7846.seq Check: 9642 from: 1 to: 1860
KIBB7846.orf
With 182 enzymes: SgfI *
November 30, 2011 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 697 1044 1860
Size: 697 347 816
Fragments arranged by size:
816 697 347
AatII G_ACGT'C
Cuts at: 0 257 286 1100 1860
Size: 257 29 814 760
Fragments arranged by size:
814 760 257 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 454 891 1417 1781 1860
Size: 205 249 437 526 364 79
Fragments arranged by size:
526 437 364 249 205 79
AfeI AGC'GCT
Cuts at: 0 994 1742 1860
Size: 994 748 118
Fragments arranged by size:
994 748 118
AflIII A'CryG_T
Cuts at: 0 1133 1860
Size: 1133 727
AhdI GACnn_n'nnGTC
Cuts at: 0 1109 1860
Size: 1109 751
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1708 1860
Size: 1708 152
AlwNI CAG_nnn'CTG
Cuts at: 0 489 1191 1860
Size: 489 702 669
Fragments arranged by size:
702 669 489
ApaI G_GGCC'C
Cuts at: 0 43 346 360 1081 1367 1860
Size: 43 303 14 721 286 493
Fragments arranged by size:
721 493 303 286 43 14
ApaLI G'TGCA_C
Cuts at: 0 763 1860
Size: 763 1097
AvaI C'yCGr_G
Cuts at: 0 78 157 172 768 888 1349 1783 1860
Size: 78 79 15 596 120 461 434 77
Fragments arranged by size:
596 461 434 120 79 78 77 15
BanI G'GyrC_C
Cuts at: 0 1280 1860
Size: 1280 580
BanII G_rGCy'C
Cuts at: 0 43 319 346 360 467 1081 1367 1507
Size: 43 276 27 14 107 614 286 140
Cuts at: 1507 1860
Size: 353
Fragments arranged by size:
614 353 286 276 140 107 43 27
14
BbvCI CC'TCA_GC
Cuts at: 0 1681 1721 1860
Size: 1681 40 139
Fragments arranged by size:
1681 139 40
BciVI GTATCCnnnnn_n'
Cuts at: 0 1217 1860
Size: 1217 643
BglI GCCn_nnn'nGGC
Cuts at: 0 392 539 560 860 1860
Size: 392 147 21 300 1000
Fragments arranged by size:
1000 392 300 147 21
BglII A'GATC_T
Cuts at: 0 333 1860
Size: 333 1527
Bme1580I G_kGCm'C
Cuts at: 0 43 346 360 767 1081 1367 1860
Size: 43 303 14 407 314 286 493
Fragments arranged by size:
493 407 314 303 286 43 14
BmgBI CAC'GTC
Cuts at: 0 1026 1136 1860
Size: 1026 110 724
Fragments arranged by size:
1026 724 110
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 338 1460 1860
Size: 338 1122 400
Fragments arranged by size:
1122 400 338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 847 900 1860
Size: 136 711 53 960
Fragments arranged by size:
960 711 136 53
Bpu10I CC'TnA_GC
Cuts at: 0 1681 1721 1860
Size: 1681 40 139
Fragments arranged by size:
1681 139 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1860
Size: 820 1040
BsaI GGTCTCn'nnnn_
Cuts at: 0 154 1630 1860
Size: 154 1476 230
Fragments arranged by size:
1476 230 154
BsaHI Gr'CG_yC
Cuts at: 0 254 283 1097 1860
Size: 254 29 814 763
Fragments arranged by size:
814 763 254 29
BsaWI w'CCGG_w
Cuts at: 0 311 1592 1860
Size: 311 1281 268
Fragments arranged by size:
1281 311 268
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 994 1024 1825 1855 1860
Size: 244 30 720 30 801 30 5
Fragments arranged by size:
801 720 244 30 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 222 237 264 643 864 1363 1860
Size: 219 3 15 27 379 221 499 497
Fragments arranged by size:
499 497 379 221 219 27 15 3
BseYI C'CCAG_C
Cuts at: 0 133 222 549 593 1006 1257 1404 1474
Size: 133 89 327 44 413 251 147 70
Cuts at: 1474 1525 1860
Size: 51 335
Fragments arranged by size:
413 335 327 251 147 133 89 70
51 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 629 1860
Size: 43 586 1231
Fragments arranged by size:
1231 586 43
BsiEI CG_ry'CG
Cuts at: 0 924 1860
Size: 924 936
BsiHKAI G_wGCw'C
Cuts at: 0 467 767 1348 1860
Size: 467 300 581 512
Fragments arranged by size:
581 512 467 300
BsiWI C'GTAC_G
Cuts at: 0 962 1860
Size: 962 898
BsmBI CGTCTCn'nnnn_
Cuts at: 0 290 1860
Size: 290 1570
Bsp1286I G_dGCh'C
Cuts at: 0 43 319 346 360 467 767 1081 1348
Size: 43 276 27 14 107 300 314 267
Cuts at: 1348 1367 1507 1860
Size: 19 140 353
Fragments arranged by size:
353 314 300 276 267 140 107 43
27 19 14
BspEI T'CCGG_A
Cuts at: 0 311 1860
Size: 311 1549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 697 1044 1860
Size: 697 347 816
Fragments arranged by size:
816 697 347
BsrBI CCG'CTC
Cuts at: 0 272 1860
Size: 272 1588
BsrFI r'CCGG_y
Cuts at: 0 1612 1860
Size: 1612 248
BstAPI GCAn_nnn'nTGC
Cuts at: 0 489 1056 1860
Size: 489 567 804
Fragments arranged by size:
804 567 489
BstEII G'GTnAC_C
Cuts at: 0 291 1537 1860
Size: 291 1246 323
Fragments arranged by size:
1246 323 291
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1146 1860
Size: 1146 714
BstYI r'GATC_y
Cuts at: 0 333 839 974 1860
Size: 333 506 135 886
Fragments arranged by size:
886 506 333 135
Bsu36I CC'TnA_GG
Cuts at: 0 847 1860
Size: 847 1013
BtgI C'CryG_G
Cuts at: 0 446 1334 1641 1860
Size: 446 888 307 219
Fragments arranged by size:
888 446 307 219
BtsI GCAGTG_nn'
Cuts at: 0 1821 1860
Size: 1821 39
DraIII CAC_nnn'GTG
Cuts at: 0 241 1860
Size: 241 1619
DrdI GACnn_nn'nnGTC
Cuts at: 0 1627 1860
Size: 1627 233
EaeI y'GGCC_r
Cuts at: 0 47 572 1860
Size: 47 525 1288
Fragments arranged by size:
1288 525 47
EarI CTCTTCn'nnn_
Cuts at: 0 646 1860
Size: 646 1214
EcoICRI GAG'CTC
Cuts at: 0 465 1860
Size: 465 1395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 205 454 847 891 900 1417 1781
Size: 136 69 249 393 44 9 517 364
Cuts at: 1781 1860
Size: 79
Fragments arranged by size:
517 393 364 249 136 79 69 44
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 436 1183 1700 1720 1860
Size: 172 264 747 517 20 140
Fragments arranged by size:
747 517 264 172 140 20
EcoO109I rG'GnC_Cy
Cuts at: 0 39 342 343 486 1078 1363 1713 1860
Size: 39 303 1 143 592 285 350 147
Fragments arranged by size:
592 350 303 285 147 143 39 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 444 1860
Size: 444 1416
HaeII r_GCGC'y
Cuts at: 0 996 1744 1860
Size: 996 748 116
Fragments arranged by size:
996 748 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 318 350 994 1026 1095 1127
Size: 244 32 42 32 644 32 69 32
Cuts at: 1127 1756 1788 1860
Size: 629 32 72
Fragments arranged by size:
644 629 244 72 69 42 32 32
32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1860
Size: 275 1585
MscI TGG'CCA
Cuts at: 0 49 1860
Size: 49 1811
MslI CAynn'nnrTG
Cuts at: 0 754 760 1860
Size: 754 6 1100
Fragments arranged by size:
1100 754 6
MspA1I CmG'CkG
Cuts at: 0 45 448 593 1146 1290 1419 1778 1860
Size: 45 403 145 553 144 129 359 82
Fragments arranged by size:
553 403 359 145 144 129 82 45
NcoI C'CATG_G
Cuts at: 0 1334 1860
Size: 1334 526
NspI r_CATG'y
Cuts at: 0 759 795 1075 1860
Size: 759 36 280 785
Fragments arranged by size:
785 759 280 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 535 1290 1827 1860
Size: 535 755 537 33
Fragments arranged by size:
755 537 535 33
PspOMI G'GGCC_C
Cuts at: 0 39 342 356 1077 1363 1860
Size: 39 303 14 721 286 497
Fragments arranged by size:
721 497 303 286 39 14
PstI C_TGCA'G
Cuts at: 0 808 1124 1860
Size: 808 316 736
Fragments arranged by size:
808 736 316
PvuII CAG'CTG
Cuts at: 0 1146 1860
Size: 1146 714
SacI G_AGCT'C
Cuts at: 0 467 1860
Size: 467 1393
SacII CC_GC'GG
Cuts at: 0 449 1860
Size: 449 1411
SapI GCTCTTCn'nnn_
Cuts at: 0 646 1860
Size: 646 1214
SfcI C'TryA_G
Cuts at: 0 804 1120 1860
Size: 804 316 740
Fragments arranged by size:
804 740 316
SmaI CCC'GGG
Cuts at: 0 80 890 1351 1860
Size: 80 810 461 509
Fragments arranged by size:
810 509 461 80
SmlI C'TyrA_G
Cuts at: 0 835 1860
Size: 835 1025
SphI G_CATG'C
Cuts at: 0 1075 1860
Size: 1075 785
StuI AGG'CCT
Cuts at: 0 542 814 904 1726 1860
Size: 542 272 90 822 134
Fragments arranged by size:
822 542 272 134 90
StyI C'CwwG_G
Cuts at: 0 534 1334 1860
Size: 534 800 526
Fragments arranged by size:
800 534 526
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 77 585 958 1120 1860
Size: 77 508 373 162 740
Fragments arranged by size:
740 508 373 162 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1306 1338 1825 1857 1860
Size: 1306 32 487 32 3
Fragments arranged by size:
1306 487 32 32 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 1860
Size: 141 1719
XmaI C'CCGG_G
Cuts at: 0 78 888 1349 1860
Size: 78 810 461 511
Fragments arranged by size:
810 511 461 78
XmnI GAAnn'nnTTC
Cuts at: 0 904 1860
Size: 904 956
ZraI GAC'GTC
Cuts at: 0 255 284 1098 1860
Size: 255 29 814 762
Fragments arranged by size:
814 762 255 29
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbvCI BciVI BglI
BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I MmeI MscI MslI MspA1I NcoI NspI PflMI
PspOMI PstI PvuII SacI SacII SapI SfcI SmaI
SmlI SphI StuI StyI TaqII TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AleI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BcgI BclI BfrBI BlpI BmrI BmtI BsaAI BsaBI
BsmI BspHI BsrDI BsrGI BssHII BssSI BstBI BstZ17I
BtgZI ClaI CspCI DraI EagI EciI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI TatI Tth111I
XbaI XhoI