Restriction map for
pFN21AB7827
(Linear) (Six-base) MAPSORT of: KIBB7827.seq Check: 3136 from: 1 to: 1614
KIBB7827.orf
With 182 enzymes: SgfI *
March 22, 2012 15:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 453 1125 1614
Size: 105 348 672 489
Fragments arranged by size:
672 489 348 105
AflII C'TTAA_G
Cuts at: 0 823 907 1614
Size: 823 84 707
Fragments arranged by size:
823 707 84
AflIII A'CryG_T
Cuts at: 0 529 958 1614
Size: 529 429 656
Fragments arranged by size:
656 529 429
AleI CACnn'nnGTG
Cuts at: 0 1268 1614
Size: 1268 346
ApoI r'AATT_y
Cuts at: 0 228 634 1233 1614
Size: 228 406 599 381
Fragments arranged by size:
599 406 381 228
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 1238 1614
Size: 39 1199 376
Fragments arranged by size:
1199 376 39
BbvCI CC'TCA_GC
Cuts at: 0 497 1614
Size: 497 1117
BmrI ACTGGGnnnn_n'
Cuts at: 0 857 1614
Size: 857 757
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 622 1614
Size: 622 992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 701 1614
Size: 133 568 913
Fragments arranged by size:
913 568 133
Bpu10I CC'TnA_GC
Cuts at: 0 497 1614
Size: 497 1117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1389 1614
Size: 1389 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 344 1353 1614
Size: 344 1009 261
Fragments arranged by size:
1009 344 261
BsaAI yAC'GTr
Cuts at: 0 594 1614
Size: 594 1020
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 73 1306 1614
Size: 70 3 1233 308
Fragments arranged by size:
1233 308 70 3
BseYI C'CCAG_C
Cuts at: 0 63 818 1614
Size: 63 755 796
Fragments arranged by size:
796 755 63
BsiHKAI G_wGCw'C
Cuts at: 0 282 1614
Size: 282 1332
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1548 1614
Size: 1548 66
Bsp1286I G_dGCh'C
Cuts at: 0 282 1614
Size: 282 1332
BspHI T'CATG_A
Cuts at: 0 810 1614
Size: 810 804
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 1614
Size: 137 1477
BsrDI GCAATG_nn'
Cuts at: 0 712 999 1614
Size: 712 287 615
Fragments arranged by size:
712 615 287
BsrGI T'GTAC_A
Cuts at: 0 1479 1614
Size: 1479 135
BstYI r'GATC_y
Cuts at: 0 242 1258 1614
Size: 242 1016 356
Fragments arranged by size:
1016 356 242
Bsu36I CC'TnA_GG
Cuts at: 0 178 1614
Size: 178 1436
BtgI C'CryG_G
Cuts at: 0 920 1614
Size: 920 694
BtsI GCAGTG_nn'
Cuts at: 0 26 1048 1431 1614
Size: 26 1022 383 183
Fragments arranged by size:
1022 383 183 26
EarI CTCTTCn'nnn_
Cuts at: 0 281 509 575 1129 1614
Size: 281 228 66 554 485
Fragments arranged by size:
554 485 281 228 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 133 453 701 1125 1614
Size: 105 28 320 248 424 489
Fragments arranged by size:
489 424 320 248 105 28
EcoRI G'AATT_C
Cuts at: 0 228 1614
Size: 228 1386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1587 1614
Size: 1587 27
HaeII r_GCGC'y
Cuts at: 0 1572 1614
Size: 1572 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 227 244 259 276 376 408 960 992
Size: 227 17 15 17 100 32 552 32
Cuts at: 992 1614
Size: 622
Fragments arranged by size:
622 552 227 100 32 32 17 17
15
HindIII A'AGCT_T
Cuts at: 0 1194 1614
Size: 1194 420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1614
Size: 236 1378
MslI CAynn'nnrTG
Cuts at: 0 696 864 948 1268 1284 1452 1536 1614
Size: 696 168 84 320 16 168 84 78
Fragments arranged by size:
696 320 168 168 84 84 78 16
NcoI C'CATG_G
Cuts at: 0 920 1614
Size: 920 694
NspI r_CATG'y
Cuts at: 0 533 794 962 1614
Size: 533 261 168 652
Fragments arranged by size:
652 533 261 168
PasI CC'CwG_GG
Cuts at: 0 444 1614
Size: 444 1170
PciI A'CATG_T
Cuts at: 0 529 958 1614
Size: 529 429 656
Fragments arranged by size:
656 529 429
PflMI CCAn_nnn'nTGG
Cuts at: 0 921 1614
Size: 921 693
PmlI CAC'GTG
Cuts at: 0 594 1614
Size: 594 1020
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 1614
Size: 118 32 1464
Fragments arranged by size:
1464 118 32
PsiI TTA'TAA
Cuts at: 0 1079 1367 1614
Size: 1079 288 247
Fragments arranged by size:
1079 288 247
SapI GCTCTTCn'nnn_
Cuts at: 0 575 1129 1614
Size: 575 554 485
Fragments arranged by size:
575 554 485
SexAI A'CCwGG_T
Cuts at: 0 334 1614
Size: 334 1280
SfcI C'TryA_G
Cuts at: 0 1515 1614
Size: 1515 99
SmlI C'TyrA_G
Cuts at: 0 823 907 1404 1614
Size: 823 84 497 210
Fragments arranged by size:
823 497 210 84
StuI AGG'CCT
Cuts at: 0 802 1614
Size: 802 812
StyI C'CwwG_G
Cuts at: 0 920 1614
Size: 920 694
TatI w'GTAC_w
Cuts at: 0 1479 1614
Size: 1479 135
XmnI GAAnn'nnTTC
Cuts at: 0 691 718 802 859 1138 1614
Size: 691 27 84 57 279 476
Fragments arranged by size:
691 476 279 84 57 27
Enzymes that do cut:
AcuI AflII AflIII AleI ApoI BbsI BbvCI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstYI
Bsu36I BtgI BtsI EarI Eco57MI EcoRI FalI HaeII
Hin4I HindIII MmeI MslI NcoI NspI PasI PciI
PflMI PmlI PpiI PsiI SapI SexAI SfcI SmlI
StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PfoI PmeI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI