Restriction map for
pFN21AB7816
(Linear) (Six-base) MAPSORT of: KIBB7816.seq Check: 3588 from: 1 to: 1788
KIBB7816.orf
With 182 enzymes: SgfI *
March 22, 2012 15:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1481 1483 1788
Size: 1481 2 305
Fragments arranged by size:
1481 305 2
AfeI AGC'GCT
Cuts at: 0 1759 1788
Size: 1759 29
AflIII A'CryG_T
Cuts at: 0 828 1788
Size: 828 960
AgeI A'CCGG_T
Cuts at: 0 575 1788
Size: 575 1213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 292 1368 1788
Size: 292 1076 420
Fragments arranged by size:
1076 420 292
AlwNI CAG_nnn'CTG
Cuts at: 0 462 559 1788
Size: 462 97 1229
Fragments arranged by size:
1229 462 97
ApaI G_GGCC'C
Cuts at: 0 533 1788
Size: 533 1255
ApaLI G'TGCA_C
Cuts at: 0 373 1788
Size: 373 1415
ApoI r'AATT_y
Cuts at: 0 1051 1788
Size: 1051 737
AvaI C'yCGr_G
Cuts at: 0 160 205 538 1788
Size: 160 45 333 1250
Fragments arranged by size:
1250 333 160 45
BanI G'GyrC_C
Cuts at: 0 56 429 1788
Size: 56 373 1359
Fragments arranged by size:
1359 373 56
BanII G_rGCy'C
Cuts at: 0 206 212 533 1788
Size: 206 6 321 1255
Fragments arranged by size:
1255 321 206 6
BbeI G_GCGC'C
Cuts at: 0 60 1788
Size: 60 1728
BbsI GAAGACnn'nnnn_
Cuts at: 0 1655 1788
Size: 1655 133
BbvCI CC'TCA_GC
Cuts at: 0 891 1788
Size: 891 897
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 884 918 1788
Size: 884 34 870
Fragments arranged by size:
884 870 34
BfrBI ATG'CAT
Cuts at: 0 711 1788
Size: 711 1077
BglI GCCn_nnn'nGGC
Cuts at: 0 403 412 528 1788
Size: 403 9 116 1260
Fragments arranged by size:
1260 403 116 9
BlpI GC'TnA_GC
Cuts at: 0 151 1400 1788
Size: 151 1249 388
Fragments arranged by size:
1249 388 151
Bme1580I G_kGCm'C
Cuts at: 0 377 434 533 1201 1788
Size: 377 57 99 668 587
Fragments arranged by size:
668 587 377 99 57
BmgBI CAC'GTC
Cuts at: 0 1740 1788
Size: 1740 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 667 1788
Size: 667 1121
BmtI G_CTAG'C
Cuts at: 0 1280 1637 1788
Size: 1280 357 151
Fragments arranged by size:
1280 357 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 757 1306 1652 1788
Size: 445 312 549 346 136
Fragments arranged by size:
549 445 346 312 136
Bpu10I CC'TnA_GC
Cuts at: 0 891 1788
Size: 891 897
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1788
Size: 364 1424
BsaAI yAC'GTr
Cuts at: 0 1576 1788
Size: 1576 212
BsaBI GATnn'nnATC
Cuts at: 0 1724 1788
Size: 1724 64
BsaHI Gr'CG_yC
Cuts at: 0 57 1788
Size: 57 1731
BsaWI w'CCGG_w
Cuts at: 0 575 1788
Size: 575 1213
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 852 1788
Size: 852 936
BseYI C'CCAG_C
Cuts at: 0 92 279 811 1788
Size: 92 187 532 977
Fragments arranged by size:
977 532 187 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1531 1788
Size: 1531 257
BsiEI CG_ry'CG
Cuts at: 0 102 419 1788
Size: 102 317 1369
Fragments arranged by size:
1369 317 102
BsiHKAI G_wGCw'C
Cuts at: 0 212 377 1472 1688 1788
Size: 212 165 1095 216 100
Fragments arranged by size:
1095 216 212 165 100
BsmI GAATG_Cn'
Cuts at: 0 713 1146 1788
Size: 713 433 642
Fragments arranged by size:
713 642 433
Bsp1286I G_dGCh'C
Cuts at: 0 206 212 377 434 533 1201 1472 1688
Size: 206 6 165 57 99 668 271 216
Cuts at: 1688 1788
Size: 100
Fragments arranged by size:
668 271 216 206 165 100 99 57
6
BsrBI CCG'CTC
Cuts at: 0 83 132 321 1788
Size: 83 49 189 1467
Fragments arranged by size:
1467 189 83 49
BsrDI GCAATG_nn'
Cuts at: 0 318 1788
Size: 318 1470
BsrFI r'CCGG_y
Cuts at: 0 303 418 575 1788
Size: 303 115 157 1213
Fragments arranged by size:
1213 303 157 115
BsrGI T'GTAC_A
Cuts at: 0 825 1788
Size: 825 963
BssSI C'ACGA_G
Cuts at: 0 370 1788
Size: 370 1418
BstEII G'GTnAC_C
Cuts at: 0 792 1788
Size: 792 996
BtgI C'CryG_G
Cuts at: 0 102 524 1788
Size: 102 422 1264
Fragments arranged by size:
1264 422 102
BtsI GCAGTG_nn'
Cuts at: 0 496 802 983 1002 1788
Size: 496 306 181 19 786
Fragments arranged by size:
786 496 306 181 19
ClaI AT'CG_AT
Cuts at: 0 122 1788
Size: 122 1666
DraIII CAC_nnn'GTG
Cuts at: 0 1542 1788
Size: 1542 246
EaeI y'GGCC_r
Cuts at: 0 99 416 1788
Size: 99 317 1372
Fragments arranged by size:
1372 317 99
EagI C'GGCC_G
Cuts at: 0 99 416 1788
Size: 99 317 1372
Fragments arranged by size:
1372 317 99
EarI CTCTTCn'nnn_
Cuts at: 0 139 217 996 1788
Size: 139 78 779 792
Fragments arranged by size:
792 779 139 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 232 337 471 1788
Size: 232 105 134 1317
Fragments arranged by size:
1317 232 134 105
EcoICRI GAG'CTC
Cuts at: 0 210 1788
Size: 210 1578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 757 1306 1481 1483 1652 1788
Size: 445 312 549 175 2 169 136
Fragments arranged by size:
549 445 312 175 169 136 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 653 1788
Size: 653 1135
EcoO109I rG'GnC_Cy
Cuts at: 0 529 530 841 1627 1788
Size: 529 1 311 786 161
Fragments arranged by size:
786 529 311 161 1
FseI GG_CCGG'CC
Cuts at: 0 422 1788
Size: 422 1366
HaeII r_GCGC'y
Cuts at: 0 10 60 387 398 1761 1788
Size: 10 50 327 11 1363 27
Fragments arranged by size:
1363 327 50 27 11 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 1303 1335 1788
Size: 583 32 688 32 453
Fragments arranged by size:
688 583 453 32 32
KasI G'GCGC_C
Cuts at: 0 56 1788
Size: 56 1732
MfeI C'AATT_G
Cuts at: 0 1065 1788
Size: 1065 723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 943 1311 1788
Size: 808 135 368 477
Fragments arranged by size:
808 477 368 135
MslI CAynn'nnrTG
Cuts at: 0 956 1788
Size: 956 832
MspA1I CmG'CkG
Cuts at: 0 92 104 366 459 526 925 1284 1788
Size: 92 12 262 93 67 399 359 504
Fragments arranged by size:
504 399 359 262 93 92 67 12
NaeI GCC'GGC
Cuts at: 0 420 1788
Size: 420 1368
NarI GG'CG_CC
Cuts at: 0 57 1788
Size: 57 1731
NgoMIV G'CCGG_C
Cuts at: 0 418 1788
Size: 418 1370
NheI G'CTAG_C
Cuts at: 0 1276 1633 1788
Size: 1276 357 155
Fragments arranged by size:
1276 357 155
NsiI A_TGCA'T
Cuts at: 0 713 1788
Size: 713 1075
NspI r_CATG'y
Cuts at: 0 832 1788
Size: 832 956
PciI A'CATG_T
Cuts at: 0 828 1788
Size: 828 960
PflMI CCAn_nnn'nTGG
Cuts at: 0 53 92 1788
Size: 53 39 1696
Fragments arranged by size:
1696 53 39
PfoI T'CCnGG_A
Cuts at: 0 714 1788
Size: 714 1074
PmlI CAC'GTG
Cuts at: 0 1576 1788
Size: 1576 212
PpuMI rG'GwC_Cy
Cuts at: 0 841 1788
Size: 841 947
PspOMI G'GGCC_C
Cuts at: 0 529 1788
Size: 529 1259
PstI C_TGCA'G
Cuts at: 0 503 809 1078 1437 1788
Size: 503 306 269 359 351
Fragments arranged by size:
503 359 351 306 269
PvuII CAG'CTG
Cuts at: 0 366 925 1284 1788
Size: 366 559 359 504
Fragments arranged by size:
559 504 366 359
SacI G_AGCT'C
Cuts at: 0 212 1788
Size: 212 1576
SacII CC_GC'GG
Cuts at: 0 105 527 1788
Size: 105 422 1261
Fragments arranged by size:
1261 422 105
SapI GCTCTTCn'nnn_
Cuts at: 0 139 217 1788
Size: 139 78 1571
Fragments arranged by size:
1571 139 78
ScaI AGT'ACT
Cuts at: 0 642 901 1080 1788
Size: 642 259 179 708
Fragments arranged by size:
708 642 259 179
SexAI A'CCwGG_T
Cuts at: 0 1538 1788
Size: 1538 250
SfcI C'TryA_G
Cuts at: 0 499 560 805 931 1074 1301 1433 1788
Size: 499 61 245 126 143 227 132 355
Fragments arranged by size:
499 355 245 227 143 132 126 61
SfoI GGC'GCC
Cuts at: 0 58 1788
Size: 58 1730
SmaI CCC'GGG
Cuts at: 0 162 1788
Size: 162 1626
SmlI C'TyrA_G
Cuts at: 0 379 1788
Size: 379 1409
SrfI GCCC'GGGC
Cuts at: 0 162 1788
Size: 162 1626
StyI C'CwwG_G
Cuts at: 0 702 1788
Size: 702 1086
TatI w'GTAC_w
Cuts at: 0 640 768 825 899 1078 1376 1788
Size: 640 128 57 74 179 298 412
Fragments arranged by size:
640 412 298 179 128 74 57
XmaI C'CCGG_G
Cuts at: 0 160 1788
Size: 160 1628
Enzymes that do cut:
AcuI AfeI AflIII AgeI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI
BsrGI BssSI BstEII BtgI BtsI ClaI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I KasI MfeI MmeI MslI MspA1I NaeI
NarI NgoMIV NheI NsiI NspI PciI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacI SacII SapI
ScaI SexAI SfcI SfoI SmaI SmlI SrfI StyI
TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AleI
AloI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BglII BplI BsaI BsaXI BsiWI BsmBI BspEI
BspHI BspMI BssHII BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI CspCI DraI DrdI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI KpnI MluI MscI
NcoI NdeI NotI NruI PacI PasI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmnI ZraI