Restriction map for
pFN21AB7802
(Linear) (Six-base) MAPSORT of: KIBB7802.seq Check: 8206 from: 1 to: 1770
KIBB7802.orf
With 182 enzymes: SgfI *
March 22, 2012 15:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1673 1770
Size: 1673 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1528 1770
Size: 1528 242
AfeI AGC'GCT
Cuts at: 0 383 733 1770
Size: 383 350 1037
Fragments arranged by size:
1037 383 350
AflIII A'CryG_T
Cuts at: 0 863 1770
Size: 863 907
AleI CACnn'nnGTG
Cuts at: 0 703 1770
Size: 703 1067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1768 1770
Size: 1768 2
AlwNI CAG_nnn'CTG
Cuts at: 0 411 435 1159 1674 1770
Size: 411 24 724 515 96
Fragments arranged by size:
724 515 411 96 24
ApaI G_GGCC'C
Cuts at: 0 834 989 1687 1739 1770
Size: 834 155 698 52 31
Fragments arranged by size:
834 698 155 52 31
ApaLI G'TGCA_C
Cuts at: 0 1608 1770
Size: 1608 162
ApoI r'AATT_y
Cuts at: 0 472 1770
Size: 472 1298
AvaI C'yCGr_G
Cuts at: 0 88 679 856 1177 1293 1404 1474 1546
Size: 88 591 177 321 116 111 70 72
Cuts at: 1546 1731 1770
Size: 185 39
Fragments arranged by size:
591 321 185 177 116 111 88 72
70 39
BamHI G'GATC_C
Cuts at: 0 1046 1443 1515 1770
Size: 1046 397 72 255
Fragments arranged by size:
1046 397 255 72
BanI G'GyrC_C
Cuts at: 0 61 105 226 312 705 821 1539 1618
Size: 61 44 121 86 393 116 718 79
Cuts at: 1618 1744 1770
Size: 126 26
Fragments arranged by size:
718 393 126 121 116 86 79 61
44 26
BanII G_rGCy'C
Cuts at: 0 834 989 1177 1307 1400 1687 1739 1770
Size: 834 155 188 130 93 287 52 31
Fragments arranged by size:
834 287 188 155 130 93 52 31
BbeI G_GCGC'C
Cuts at: 0 65 109 230 316 825 1543 1770
Size: 65 44 121 86 509 718 227
Fragments arranged by size:
718 509 227 121 86 65 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 390 537 909 1770
Size: 390 147 372 861
Fragments arranged by size:
861 390 372 147
BbvCI CC'TCA_GC
Cuts at: 0 268 1770
Size: 268 1502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 528 562 1770
Size: 528 34 1208
Fragments arranged by size:
1208 528 34
BglI GCCn_nnn'nGGC
Cuts at: 0 324 371 883 1156 1270 1770
Size: 324 47 512 273 114 500
Fragments arranged by size:
512 500 324 273 114 47
BlpI GC'TnA_GC
Cuts at: 0 887 1213 1770
Size: 887 326 557
Fragments arranged by size:
887 557 326
Bme1580I G_kGCm'C
Cuts at: 0 710 834 863 989 1612 1687 1739 1747
Size: 710 124 29 126 623 75 52 8
Cuts at: 1747 1770
Size: 23
Fragments arranged by size:
710 623 126 124 75 52 29 23
8
BmgBI CAC'GTC
Cuts at: 0 783 1770
Size: 783 987
BmrI ACTGGGnnnn_n'
Cuts at: 0 601 827 1770
Size: 601 226 943
Fragments arranged by size:
943 601 226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 513 1464 1500 1770
Size: 513 951 36 270
Fragments arranged by size:
951 513 270 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 219 604 1434 1770
Size: 208 11 385 830 336
Fragments arranged by size:
830 385 336 208 11
Bpu10I CC'TnA_GC
Cuts at: 0 268 1770
Size: 268 1502
BsaI GGTCTCn'nnnn_
Cuts at: 0 393 517 940 1770
Size: 393 124 423 830
Fragments arranged by size:
830 423 393 124
BsaAI yAC'GTr
Cuts at: 0 813 1062 1209 1770
Size: 813 249 147 561
Fragments arranged by size:
813 561 249 147
BsaHI Gr'CG_yC
Cuts at: 0 62 106 227 313 604 610 822 1037
Size: 62 44 121 86 291 6 212 215
Cuts at: 1037 1540 1770
Size: 503 230
Fragments arranged by size:
503 291 230 215 212 121 86 62
44 6
BsaWI w'CCGG_w
Cuts at: 0 112 1518 1770
Size: 112 1406 252
Fragments arranged by size:
1406 252 112
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 630 639 642 705 1294 1770
Size: 630 9 3 63 589 476
Fragments arranged by size:
630 589 476 63 9 3
BseYI C'CCAG_C
Cuts at: 0 306 1159 1347 1644 1674 1770
Size: 306 853 188 297 30 96
Fragments arranged by size:
853 306 297 188 96 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1555 1770
Size: 763 792 215
Fragments arranged by size:
792 763 215
BsiHKAI G_wGCw'C
Cuts at: 0 1187 1214 1612 1770
Size: 1187 27 398 158
Fragments arranged by size:
1187 398 158 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 642 928 1770
Size: 642 286 842
Fragments arranged by size:
842 642 286
Bsp1286I G_dGCh'C
Cuts at: 0 710 834 863 989 1177 1187 1214 1307
Size: 710 124 29 126 188 10 27 93
Cuts at: 1307 1400 1612 1687 1739 1747 1770
Size: 93 212 75 52 8 23
Fragments arranged by size:
710 212 188 126 124 93 93 75
52 29 27 23 10 8
BspEI T'CCGG_A
Cuts at: 0 112 1770
Size: 112 1658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1673 1770
Size: 1673 97
BsrBI CCG'CTC
Cuts at: 0 1166 1770
Size: 1166 604
BsrFI r'CCGG_y
Cuts at: 0 293 1590 1770
Size: 293 1297 180
Fragments arranged by size:
1297 293 180
BssHII G'CGCG_C
Cuts at: 0 160 1770
Size: 160 1610
BssSI C'ACGA_G
Cuts at: 0 1041 1770
Size: 1041 729
BstAPI GCAn_nnn'nTGC
Cuts at: 0 867 1191 1770
Size: 867 324 579
Fragments arranged by size:
867 579 324
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 507 817 1770
Size: 243 264 310 953
Fragments arranged by size:
953 310 264 243
BstYI r'GATC_y
Cuts at: 0 1046 1443 1515 1770
Size: 1046 397 72 255
Fragments arranged by size:
1046 397 255 72
Bsu36I CC'TnA_GG
Cuts at: 0 792 1770
Size: 792 978
BtgI C'CryG_G
Cuts at: 0 57 335 369 900 1026 1528 1637 1739
Size: 57 278 34 531 126 502 109 102
Cuts at: 1739 1756 1770
Size: 17 14
Fragments arranged by size:
531 502 278 126 109 102 57 34
17 14
BtsI GCAGTG_nn'
Cuts at: 0 27 1770
Size: 27 1743
DraIII CAC_nnn'GTG
Cuts at: 0 1191 1770
Size: 1191 579
EaeI y'GGCC_r
Cuts at: 0 203 1148 1230 1460 1525 1770
Size: 203 945 82 230 65 245
Fragments arranged by size:
945 245 230 203 82 65
EarI CTCTTCn'nnn_
Cuts at: 0 648 660 666 1165 1770
Size: 648 12 6 499 605
Fragments arranged by size:
648 605 499 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 44 274 1039 1770
Size: 44 230 765 731
Fragments arranged by size:
765 731 230 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 219 604 1434 1528 1770
Size: 208 11 385 830 94 242
Fragments arranged by size:
830 385 242 208 94 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 700 724 1510 1770
Size: 700 24 786 260
Fragments arranged by size:
786 700 260 24
EcoO109I rG'GnC_Cy
Cuts at: 0 985 986 1362 1503 1533 1551 1602 1684
Size: 985 1 376 141 30 18 51 82
Cuts at: 1684 1735 1770
Size: 51 35
Fragments arranged by size:
985 376 141 82 51 51 35 30
18 1
HaeII r_GCGC'y
Cuts at: 0 65 109 230 316 385 735 825 1543
Size: 65 44 121 86 69 350 90 718
Cuts at: 1543 1770
Size: 227
Fragments arranged by size:
718 350 227 121 90 86 69 65
44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 407 439 896 928 1270 1302 1662 1694
Size: 407 32 457 32 342 32 360 32
Cuts at: 1694 1770
Size: 76
Fragments arranged by size:
457 407 360 342 76 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 208 1770
Size: 208 1562
KasI G'GCGC_C
Cuts at: 0 61 105 226 312 821 1539 1770
Size: 61 44 121 86 509 718 231
Fragments arranged by size:
718 509 231 121 86 61 44
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1348 1770
Size: 1348 422
MscI TGG'CCA
Cuts at: 0 205 1150 1232 1770
Size: 205 945 82 538
Fragments arranged by size:
945 538 205 82
MslI CAynn'nnrTG
Cuts at: 0 703 811 1390 1770
Size: 703 108 579 380
Fragments arranged by size:
703 579 380 108
MspA1I CmG'CkG
Cuts at: 0 59 219 243 337 349 371 1010 1351
Size: 59 160 24 94 12 22 639 341
Cuts at: 1351 1639 1741 1758 1770
Size: 288 102 17 12
Fragments arranged by size:
639 341 288 160 102 94 59 24
22 17 12 12
NarI GG'CG_CC
Cuts at: 0 62 106 227 313 822 1540 1770
Size: 62 44 121 86 509 718 230
Fragments arranged by size:
718 509 230 121 86 62 44
NcoI C'CATG_G
Cuts at: 0 900 1528 1770
Size: 900 628 242
Fragments arranged by size:
900 628 242
PasI CC'CwG_GG
Cuts at: 0 74 320 562 840 1374 1716 1770
Size: 74 246 242 278 534 342 54
Fragments arranged by size:
534 342 278 246 242 74 54
PflMI CCAn_nnn'nTGG
Cuts at: 0 840 901 1770
Size: 840 61 869
Fragments arranged by size:
869 840 61
PfoI T'CCnGG_A
Cuts at: 0 148 725 1694 1770
Size: 148 577 969 76
Fragments arranged by size:
969 577 148 76
PmlI CAC'GTG
Cuts at: 0 813 1062 1209 1770
Size: 813 249 147 561
Fragments arranged by size:
813 561 249 147
PpuMI rG'GwC_Cy
Cuts at: 0 1503 1551 1770
Size: 1503 48 219
Fragments arranged by size:
1503 219 48
PshAI GACnn'nnGTC
Cuts at: 0 1079 1770
Size: 1079 691
PspOMI G'GGCC_C
Cuts at: 0 830 985 1683 1735 1770
Size: 830 155 698 52 35
Fragments arranged by size:
830 698 155 52 35
PstI C_TGCA'G
Cuts at: 0 128 1115 1142 1670 1770
Size: 128 987 27 528 100
Fragments arranged by size:
987 528 128 100 27
PvuII CAG'CTG
Cuts at: 0 243 349 1010 1770
Size: 243 106 661 760
Fragments arranged by size:
760 661 243 106
RsrII CG'GwC_CG
Cuts at: 0 115 1770
Size: 115 1655
SacII CC_GC'GG
Cuts at: 0 60 338 372 1640 1742 1759 1770
Size: 60 278 34 1268 102 17 11
Fragments arranged by size:
1268 278 102 60 34 17 11
SanDI GG'GwC_CC
Cuts at: 0 1551 1770
Size: 1551 219
SapI GCTCTTCn'nnn_
Cuts at: 0 1165 1770
Size: 1165 605
SbfI CC_TGCA'GG
Cuts at: 0 128 1770
Size: 128 1642
SfcI C'TryA_G
Cuts at: 0 124 1111 1138 1666 1770
Size: 124 987 27 528 104
Fragments arranged by size:
987 528 124 104 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 371 1770
Size: 371 1399
SfoI GGC'GCC
Cuts at: 0 63 107 228 314 823 1541 1770
Size: 63 44 121 86 509 718 229
Fragments arranged by size:
718 509 229 121 86 63 44
SgrAI Cr'CCGG_yG
Cuts at: 0 1590 1770
Size: 1590 180
SmaI CCC'GGG
Cuts at: 0 90 1179 1295 1548 1733 1770
Size: 90 1089 116 253 185 37
Fragments arranged by size:
1089 253 185 116 90 37
StuI AGG'CCT
Cuts at: 0 790 1702 1770
Size: 790 912 68
Fragments arranged by size:
912 790 68
StyI C'CwwG_G
Cuts at: 0 701 900 1528 1770
Size: 701 199 628 242
Fragments arranged by size:
701 628 242 199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1225 1770
Size: 1225 545
XmaI C'CCGG_G
Cuts at: 0 88 1177 1293 1546 1731 1770
Size: 88 1089 116 253 185 39
Fragments arranged by size:
1089 253 185 116 88 39
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI
NcoI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PstI PvuII RsrII SacII SanDI SapI SbfI SfcI
SfiI SfoI SgrAI SmaI StuI StyI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI
BglII BmtI BpuEI BsaBI BsaXI BsiEI BsiWI BsmI
BspHI BsrDI BsrGI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI
SacI SalI ScaI SexAI SgfI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmnI ZraI