Restriction map for
pFN21AB7796
(Linear) (Six-base) MAPSORT of: KIBB7796.seq Check: 6861 from: 1 to: 1554
KIBB7796.orf
With 182 enzymes: SgfI *
December 8, 2011 13:20 ..
Acc65I G'GTAC_C
Cuts at: 0 21 1554
Size: 21 1533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 652 1554
Size: 433 219 902
Fragments arranged by size:
902 433 219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 976 1554
Size: 976 578
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 608 640 1554
Size: 608 32 914
Fragments arranged by size:
914 608 32
AlwNI CAG_nnn'CTG
Cuts at: 0 978 1021 1554
Size: 978 43 533
Fragments arranged by size:
978 533 43
ApaLI G'TGCA_C
Cuts at: 0 961 1554
Size: 961 593
BanI G'GyrC_C
Cuts at: 0 21 1101 1179 1407 1554
Size: 21 1080 78 228 147
Fragments arranged by size:
1080 228 147 78 21
BanII G_rGCy'C
Cuts at: 0 601 1056 1554
Size: 601 455 498
Fragments arranged by size:
601 498 455
BbeI G_GCGC'C
Cuts at: 0 1105 1554
Size: 1105 449
BbsI GAAGACnn'nnnn_
Cuts at: 0 123 1554
Size: 123 1431
BbvCI CC'TCA_GC
Cuts at: 0 971 1554
Size: 971 583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 15 49 1554
Size: 15 34 1505
Fragments arranged by size:
1505 34 15
BglI GCCn_nnn'nGGC
Cuts at: 0 1388 1554
Size: 1388 166
Bme1580I G_kGCm'C
Cuts at: 0 425 446 965 1184 1412 1554
Size: 425 21 519 219 228 142
Fragments arranged by size:
519 425 228 219 142 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 1166 1554
Size: 1166 388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 709 1053 1554
Size: 700 9 344 501
Fragments arranged by size:
700 501 344 9
Bpu10I CC'TnA_GC
Cuts at: 0 971 1554
Size: 971 583
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 1554
Size: 268 1286
BsaBI GATnn'nnATC
Cuts at: 0 1156 1554
Size: 1156 398
BsaHI Gr'CG_yC
Cuts at: 0 1102 1417 1554
Size: 1102 315 137
Fragments arranged by size:
1102 315 137
BsaWI w'CCGG_w
Cuts at: 0 333 1554
Size: 333 1221
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 608 638 1051 1081 1554
Size: 265 30 313 30 413 30 473
Fragments arranged by size:
473 413 313 265 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 511 514 697 751 1133 1554
Size: 370 141 3 183 54 382 421
Fragments arranged by size:
421 382 370 183 141 54 3
BseYI C'CCAG_C
Cuts at: 0 120 389 1554
Size: 120 269 1165
Fragments arranged by size:
1165 269 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1554
Size: 796 758
BsiEI CG_ry'CG
Cuts at: 0 40 671 1554
Size: 40 631 883
Fragments arranged by size:
883 631 40
BsiHKAI G_wGCw'C
Cuts at: 0 473 965 1554
Size: 473 492 589
Fragments arranged by size:
589 492 473
BsmBI CGTCTCn'nnnn_
Cuts at: 0 252 1554
Size: 252 1302
Bsp1286I G_dGCh'C
Cuts at: 0 425 446 473 601 965 1056 1184 1412
Size: 425 21 27 128 364 91 128 228
Cuts at: 1412 1554
Size: 142
Fragments arranged by size:
425 364 228 142 128 128 91 27
21
BspEI T'CCGG_A
Cuts at: 0 333 1554
Size: 333 1221
BspHI T'CATG_A
Cuts at: 0 1088 1554
Size: 1088 466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 768 1554
Size: 768 786
BsrFI r'CCGG_y
Cuts at: 0 39 584 877 965 1342 1366 1554
Size: 39 545 293 88 377 24 188
Fragments arranged by size:
545 377 293 188 88 39 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1021 1554
Size: 1021 533
BstXI CCAn_nnnn'nTGG
Cuts at: 0 324 834 1539 1554
Size: 324 510 705 15
Fragments arranged by size:
705 510 324 15
BstYI r'GATC_y
Cuts at: 0 395 1554
Size: 395 1159
Bsu36I CC'TnA_GG
Cuts at: 0 1145 1554
Size: 1145 409
BtgI C'CryG_G
Cuts at: 0 192 237 1361 1554
Size: 192 45 1124 193
Fragments arranged by size:
1124 193 192 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 78 113 1554
Size: 78 35 1441
Fragments arranged by size:
1441 78 35
EaeI y'GGCC_r
Cuts at: 0 37 99 668 1238 1364 1380 1389 1554
Size: 37 62 569 570 126 16 9 165
Fragments arranged by size:
570 569 165 126 62 37 16 9
EagI C'GGCC_G
Cuts at: 0 37 668 1554
Size: 37 631 886
Fragments arranged by size:
886 631 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 1554
Size: 154 1400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 652 700 709 1053 1554
Size: 433 219 48 9 344 501
Fragments arranged by size:
501 433 344 219 48 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 702 1554
Size: 702 852
EcoO109I rG'GnC_Cy
Cuts at: 0 707 1451 1554
Size: 707 744 103
Fragments arranged by size:
744 707 103
HaeII r_GCGC'y
Cuts at: 0 1105 1554
Size: 1105 449
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 583 595 615 627 1024 1056
Size: 265 32 286 12 20 12 397 32
Cuts at: 1056 1554
Size: 498
Fragments arranged by size:
498 397 286 265 32 32 20 12
12
KasI G'GCGC_C
Cuts at: 0 1101 1554
Size: 1101 453
KpnI G_GTAC'C
Cuts at: 0 25 1554
Size: 25 1529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 634 1133 1554
Size: 593 41 499 421
Fragments arranged by size:
593 499 421 41
MscI TGG'CCA
Cuts at: 0 101 1554
Size: 101 1453
MslI CAynn'nnrTG
Cuts at: 0 880 1554
Size: 880 674
MspA1I CmG'CkG
Cuts at: 0 120 225 389 678 834 906 1018 1218
Size: 120 105 164 289 156 72 112 200
Cuts at: 1218 1259 1269 1554
Size: 41 10 285
Fragments arranged by size:
289 285 200 164 156 120 112 105
72 41 10
NaeI GCC'GGC
Cuts at: 0 41 1368 1554
Size: 41 1327 186
Fragments arranged by size:
1327 186 41
NarI GG'CG_CC
Cuts at: 0 1102 1554
Size: 1102 452
NcoI C'CATG_G
Cuts at: 0 192 1554
Size: 192 1362
NgoMIV G'CCGG_C
Cuts at: 0 39 1366 1554
Size: 39 1327 188
Fragments arranged by size:
1327 188 39
NspI r_CATG'y
Cuts at: 0 384 885 1554
Size: 384 501 669
Fragments arranged by size:
669 501 384
PasI CC'CwG_GG
Cuts at: 0 1412 1448 1554
Size: 1412 36 106
Fragments arranged by size:
1412 106 36
PfoI T'CCnGG_A
Cuts at: 0 398 1554
Size: 398 1156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 608 640 1554
Size: 608 32 914
Fragments arranged by size:
914 608 32
PpuMI rG'GwC_Cy
Cuts at: 0 707 1554
Size: 707 847
PstI C_TGCA'G
Cuts at: 0 1017 1026 1264 1554
Size: 1017 9 238 290
Fragments arranged by size:
1017 290 238 9
PvuII CAG'CTG
Cuts at: 0 120 225 389 678 834 906 1259 1554
Size: 120 105 164 289 156 72 353 295
Fragments arranged by size:
353 295 289 164 156 120 105 72
SfcI C'TryA_G
Cuts at: 0 1013 1022 1260 1332 1347 1554
Size: 1013 9 238 72 15 207
Fragments arranged by size:
1013 238 207 72 15 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1388 1554
Size: 1388 166
SfoI GGC'GCC
Cuts at: 0 1103 1554
Size: 1103 451
SphI G_CATG'C
Cuts at: 0 885 1554
Size: 885 669
StuI AGG'CCT
Cuts at: 0 697 1315 1554
Size: 697 618 239
Fragments arranged by size:
697 618 239
StyI C'CwwG_G
Cuts at: 0 102 192 1554
Size: 102 90 1362
Fragments arranged by size:
1362 102 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 446 888 1341 1554
Size: 446 442 453 213
Fragments arranged by size:
453 446 442 213
TatI w'GTAC_w
Cuts at: 0 359 1554
Size: 359 1195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1554
Size: 1051 32 471
Fragments arranged by size:
1051 471 32
Tth111I GACn'n_nGTC
Cuts at: 0 279 650 1554
Size: 279 371 904
Fragments arranged by size:
904 371 279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1512 1554
Size: 1512 42
Enzymes that do cut:
Acc65I AcuI AlfI AloI AlwNI ApaLI BanI BanII
BbeI BbsI BbvCI BcgI BglI Bme1580I BmrI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrFI BstAPI BstXI BstYI Bsu36I BtgI CspCI EaeI
EagI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PfoI PpiI PpuMI PstI PvuII
SfcI SfiI SfoI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BpuEI BsaAI BsiWI BsmI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
BtsI ClaI DraI DraIII DrdI EarI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI XmaI XmnI
ZraI