Restriction map for
pFN21AB7790
(Linear) (Six-base) MAPSORT of: KIBB7790.seq Check: 90 from: 1 to: 1551
KIBB7790.orf
With 182 enzymes: SgfI *
March 22, 2012 15:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 773 1310 1314 1551
Size: 323 450 537 4 237
Fragments arranged by size:
537 450 323 237 4
AflII C'TTAA_G
Cuts at: 0 646 1551
Size: 646 905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1317 1551
Size: 1317 234
AlwNI CAG_nnn'CTG
Cuts at: 0 9 1551
Size: 9 1542
ApaI G_GGCC'C
Cuts at: 0 185 1551
Size: 185 1366
ApoI r'AATT_y
Cuts at: 0 324 514 600 846 971 1024 1128 1551
Size: 324 190 86 246 125 53 104 423
Fragments arranged by size:
423 324 246 190 125 104 86 53
AseI AT'TA_AT
Cuts at: 0 935 1551
Size: 935 616
AvaI C'yCGr_G
Cuts at: 0 1538 1551
Size: 1538 13
BanII G_rGCy'C
Cuts at: 0 185 215 1423 1551
Size: 185 30 1208 128
Fragments arranged by size:
1208 185 128 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 706 1349 1551
Size: 211 495 643 202
Fragments arranged by size:
643 495 211 202
Bme1580I G_kGCm'C
Cuts at: 0 185 1551
Size: 185 1366
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1442 1551
Size: 1442 109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1551
Size: 749 802
Bpu10I CC'TnA_GC
Cuts at: 0 196 1551
Size: 196 1355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1101 1551
Size: 1101 450
BsaI GGTCTCn'nnnn_
Cuts at: 0 225 995 1551
Size: 225 770 556
Fragments arranged by size:
770 556 225
BsaAI yAC'GTr
Cuts at: 0 55 1551
Size: 55 1496
BsaBI GATnn'nnATC
Cuts at: 0 25 1551
Size: 25 1526
BsaWI w'CCGG_w
Cuts at: 0 1415 1551
Size: 1415 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1405 1435 1551
Size: 1405 30 116
Fragments arranged by size:
1405 116 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 904 1413 1551
Size: 904 509 138
Fragments arranged by size:
904 509 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1287 1551
Size: 1287 264
BsiHKAI G_wGCw'C
Cuts at: 0 215 1423 1551
Size: 215 1208 128
Fragments arranged by size:
1208 215 128
Bsp1286I G_dGCh'C
Cuts at: 0 185 215 1423 1551
Size: 185 30 1208 128
Fragments arranged by size:
1208 185 128 30
BsrDI GCAATG_nn'
Cuts at: 0 604 1551
Size: 604 947
BsrGI T'GTAC_A
Cuts at: 0 563 1551
Size: 563 988
BstEII G'GTnAC_C
Cuts at: 0 357 778 1551
Size: 357 421 773
Fragments arranged by size:
773 421 357
BtgI C'CryG_G
Cuts at: 0 744 1551
Size: 744 807
BtsI GCAGTG_nn'
Cuts at: 0 1233 1551
Size: 1233 318
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1299 1334 1551
Size: 1299 35 217
Fragments arranged by size:
1299 217 35
DraI TTT'AAA
Cuts at: 0 1011 1551
Size: 1011 540
EarI CTCTTCn'nnn_
Cuts at: 0 521 712 1551
Size: 521 191 839
Fragments arranged by size:
839 521 191
EcoICRI GAG'CTC
Cuts at: 0 213 1421 1551
Size: 213 1208 130
Fragments arranged by size:
1208 213 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 749 773 1310 1314 1551
Size: 323 426 24 537 4 237
Fragments arranged by size:
537 426 323 237 24 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1497 1551
Size: 1497 54
EcoO109I rG'GnC_Cy
Cuts at: 0 182 1551
Size: 182 1369
EcoRI G'AATT_C
Cuts at: 0 514 600 971 1128 1551
Size: 514 86 371 157 423
Fragments arranged by size:
514 423 371 157 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 942 1551
Size: 942 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1178 1210 1551
Size: 1178 32 341
Fragments arranged by size:
1178 341 32
HincII GTy'rAC
Cuts at: 0 1395 1551
Size: 1395 156
HindIII A'AGCT_T
Cuts at: 0 924 1551
Size: 924 627
HpaI GTT'AAC
Cuts at: 0 1395 1551
Size: 1395 156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1381 1551
Size: 632 749 170
Fragments arranged by size:
749 632 170
MslI CAynn'nnrTG
Cuts at: 0 1470 1551
Size: 1470 81
NcoI C'CATG_G
Cuts at: 0 744 1551
Size: 744 807
PspOMI G'GGCC_C
Cuts at: 0 181 1551
Size: 181 1370
PstI C_TGCA'G
Cuts at: 0 428 1551
Size: 428 1123
SacI G_AGCT'C
Cuts at: 0 215 1423 1551
Size: 215 1208 128
Fragments arranged by size:
1208 215 128
SfcI C'TryA_G
Cuts at: 0 424 1551
Size: 424 1127
SmlI C'TyrA_G
Cuts at: 0 646 1080 1538 1551
Size: 646 434 458 13
Fragments arranged by size:
646 458 434 13
StuI AGG'CCT
Cuts at: 0 1300 1327 1551
Size: 1300 27 224
Fragments arranged by size:
1300 224 27
StyI C'CwwG_G
Cuts at: 0 694 744 1551
Size: 694 50 807
Fragments arranged by size:
807 694 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 309 1551
Size: 309 1242
TatI w'GTAC_w
Cuts at: 0 563 1090 1551
Size: 563 527 461
Fragments arranged by size:
563 527 461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 1551
Size: 111 1440
XhoI C'TCGA_G
Cuts at: 0 1538 1551
Size: 1538 13
XmnI GAAnn'nnTTC
Cuts at: 0 975 1159 1465 1551
Size: 975 184 306 86
Fragments arranged by size:
975 306 184 86
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApaI ApoI AseI AvaI
BanII BbsI Bme1580I BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BsrDI BsrGI BstEII BtgI BtsI CspCI DraI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII
HindIII HpaI MmeI MslI NcoI PspOMI PstI SacI
SfcI SmlI StuI StyI TaqII TatI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BsaHI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI DraIII DrdI EaeI EagI EciI
EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XmaI ZraI