Restriction map for
pFN21AB7761
(Linear) (Six-base) MAPSORT of: KIBB7761.seq Check: 244 from: 1 to: 1344
KIBB7761.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AccI GT'mk_AC
Cuts at: 0 365 1344
Size: 365 979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 106 408 1344
Size: 64 42 302 936
Fragments arranged by size:
936 302 64 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 961 1344
Size: 961 383
ApaI G_GGCC'C
Cuts at: 0 399 1049 1344
Size: 399 650 295
Fragments arranged by size:
650 399 295
ApaLI G'TGCA_C
Cuts at: 0 762 1117 1344
Size: 762 355 227
Fragments arranged by size:
762 355 227
ApoI r'AATT_y
Cuts at: 0 477 918 1344
Size: 477 441 426
Fragments arranged by size:
477 441 426
AvaI C'yCGr_G
Cuts at: 0 957 1344
Size: 957 387
BanI G'GyrC_C
Cuts at: 0 324 1084 1316 1344
Size: 324 760 232 28
Fragments arranged by size:
760 324 232 28
BanII G_rGCy'C
Cuts at: 0 399 1049 1232 1344
Size: 399 650 183 112
Fragments arranged by size:
650 399 183 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1184 1218 1256 1290 1344
Size: 1184 34 38 34 54
Fragments arranged by size:
1184 54 38 34 34
Bme1580I G_kGCm'C
Cuts at: 0 22 329 399 531 766 1049 1121 1319
Size: 22 307 70 132 235 283 72 198
Cuts at: 1319 1344
Size: 25
Fragments arranged by size:
307 283 235 198 132 72 70 25
22
BmrI ACTGGGnnnn_n'
Cuts at: 0 561 1091 1344
Size: 561 530 253
Fragments arranged by size:
561 530 253
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 711 1159 1344
Size: 711 448 185
Fragments arranged by size:
711 448 185
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 333 1344
Size: 154 179 1011
Fragments arranged by size:
1011 179 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 1344
Size: 918 426
BsaI GGTCTCn'nnnn_
Cuts at: 0 351 856 1344
Size: 351 505 488
Fragments arranged by size:
505 488 351
BsaWI w'CCGG_w
Cuts at: 0 684 1344
Size: 684 660
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 364 394 1344
Size: 364 30 950
Fragments arranged by size:
950 364 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 613 788 1135 1344
Size: 613 175 347 209
Fragments arranged by size:
613 347 209 175
BseYI C'CCAG_C
Cuts at: 0 15 31 1048 1155 1292 1344
Size: 15 16 1017 107 137 52
Fragments arranged by size:
1017 137 107 52 16 15
BsiHKAI G_wGCw'C
Cuts at: 0 766 842 1121 1184 1344
Size: 766 76 279 63 160
Fragments arranged by size:
766 279 160 76 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 671 1344
Size: 671 673
Bsp1286I G_dGCh'C
Cuts at: 0 22 329 399 531 766 842 1049 1121
Size: 22 307 70 132 235 76 207 72
Cuts at: 1121 1184 1232 1319 1344
Size: 63 48 87 25
Fragments arranged by size:
307 235 207 132 87 76 72 70
63 48 25 22
BsrBI CCG'CTC
Cuts at: 0 796 1309 1344
Size: 796 513 35
Fragments arranged by size:
796 513 35
BsrDI GCAATG_nn'
Cuts at: 0 1086 1244 1344
Size: 1086 158 100
Fragments arranged by size:
1086 158 100
BsrFI r'CCGG_y
Cuts at: 0 863 1344
Size: 863 481
BstYI r'GATC_y
Cuts at: 0 737 1344
Size: 737 607
BtgI C'CryG_G
Cuts at: 0 828 1344
Size: 828 516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 50 1344
Size: 50 1294
BtsI GCAGTG_nn'
Cuts at: 0 1274 1344
Size: 1274 70
DraIII CAC_nnn'GTG
Cuts at: 0 513 1344
Size: 513 831
EaeI y'GGCC_r
Cuts at: 0 2 1344
Size: 2 1342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 106 154 333 408 1344
Size: 64 42 48 179 75 936
Fragments arranged by size:
936 179 75 64 48 42
EcoO109I rG'GnC_Cy
Cuts at: 0 395 961 1045 1093 1344
Size: 395 566 84 48 251
Fragments arranged by size:
566 395 251 84 48
EcoRI G'AATT_C
Cuts at: 0 477 1344
Size: 477 867
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 396 428 573 594 605 626 705 722
Size: 396 32 145 21 11 21 79 17
Cuts at: 722 737 754 1344
Size: 15 17 590
Fragments arranged by size:
590 396 145 79 32 21 21 17
17 15 11
HincII GTy'rAC
Cuts at: 0 312 1344
Size: 312 1032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 501 1344
Size: 221 280 843
Fragments arranged by size:
843 280 221
MscI TGG'CCA
Cuts at: 0 4 1344
Size: 4 1340
MslI CAynn'nnrTG
Cuts at: 0 491 577 833 1249 1344
Size: 491 86 256 416 95
Fragments arranged by size:
491 416 256 95 86
MspA1I CmG'CkG
Cuts at: 0 299 331 359 971 1159 1344
Size: 299 32 28 612 188 185
Fragments arranged by size:
612 299 188 185 32 28
NcoI C'CATG_G
Cuts at: 0 828 1344
Size: 828 516
NspI r_CATG'y
Cuts at: 0 712 894 1344
Size: 712 182 450
Fragments arranged by size:
712 450 182
PflMI CCAn_nnn'nTGG
Cuts at: 0 537 1344
Size: 537 807
PfoI T'CCnGG_A
Cuts at: 0 131 1344
Size: 131 1213
PpuMI rG'GwC_Cy
Cuts at: 0 961 1344
Size: 961 383
PspOMI G'GGCC_C
Cuts at: 0 395 1045 1344
Size: 395 650 299
Fragments arranged by size:
650 395 299
PvuII CAG'CTG
Cuts at: 0 359 971 1159 1344
Size: 359 612 188 185
Fragments arranged by size:
612 359 188 185
SmlI C'TyrA_G
Cuts at: 0 933 1344
Size: 933 411
StyI C'CwwG_G
Cuts at: 0 656 828 1344
Size: 656 172 516
Fragments arranged by size:
656 516 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 838 1204 1212 1344
Size: 312 526 366 8 132
Fragments arranged by size:
526 366 312 132 8
TatI w'GTAC_w
Cuts at: 0 257 623 1344
Size: 257 366 721
Fragments arranged by size:
721 366 257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 364 396 1344
Size: 364 32 948
Fragments arranged by size:
948 364 32
Tth111I GACn'n_nGTC
Cuts at: 0 277 1344
Size: 277 1067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 645 826 1344
Size: 12 633 181 518
Fragments arranged by size:
633 518 181 12
XmnI GAAnn'nnTTC
Cuts at: 0 903 1344
Size: 903 441
Enzymes that do cut:
AccI AcuI AlfI ApaI ApaLI ApoI AvaI BanI
BanII BcgI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BsrBI
BsrDI BsrFI BstYI BtgI BtgZI BtsI DraIII EaeI
Eco57MI EcoO109I EcoRI Hin4I HincII MmeI MscI MslI
MspA1I NcoI NspI PflMI PfoI PpuMI PspOMI PvuII
SmlI StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaBI
BsaHI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI XmaI ZraI