Restriction map for
pFN21AB7733
(Linear) (Six-base) MAPSORT of: KIBB7733.seq Check: 7891 from: 1 to: 1299
KIBB7733.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 396 1299
Size: 396 903
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1113 1299
Size: 710 403 186
Fragments arranged by size:
710 403 186
AflII C'TTAA_G
Cuts at: 0 447 1299
Size: 447 852
AflIII A'CryG_T
Cuts at: 0 889 1189 1299
Size: 889 300 110
Fragments arranged by size:
889 300 110
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 1299
Size: 80 32 1187
Fragments arranged by size:
1187 80 32
ApoI r'AATT_y
Cuts at: 0 63 882 1299
Size: 63 819 417
Fragments arranged by size:
819 417 63
AvaI C'yCGr_G
Cuts at: 0 608 1027 1299
Size: 608 419 272
Fragments arranged by size:
608 419 272
AvrII C'CTAG_G
Cuts at: 0 682 1299
Size: 682 617
BamHI G'GATC_C
Cuts at: 0 910 1299
Size: 910 389
BbsI GAAGACnn'nnnn_
Cuts at: 0 365 1002 1299
Size: 365 637 297
Fragments arranged by size:
637 365 297
BglII A'GATC_T
Cuts at: 0 9 754 1299
Size: 9 745 545
Fragments arranged by size:
745 545 9
BmgBI CAC'GTC
Cuts at: 0 402 1299
Size: 402 897
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 858 1299
Size: 858 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1299
Size: 286 1013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 1299
Size: 111 1188
BsaI GGTCTCn'nnnn_
Cuts at: 0 1157 1299
Size: 1157 142
BsiHKAI G_wGCw'C
Cuts at: 0 140 1299
Size: 140 1159
BsmI GAATG_Cn'
Cuts at: 0 489 952 1299
Size: 489 463 347
Fragments arranged by size:
489 463 347
Bsp1286I G_dGCh'C
Cuts at: 0 140 1299
Size: 140 1159
BspHI T'CATG_A
Cuts at: 0 1155 1251 1299
Size: 1155 96 48
Fragments arranged by size:
1155 96 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 99 396 1154 1299
Size: 99 297 758 145
Fragments arranged by size:
758 297 145 99
BsrDI GCAATG_nn'
Cuts at: 0 300 879 1299
Size: 300 579 420
Fragments arranged by size:
579 420 300
BstAPI GCAn_nnn'nTGC
Cuts at: 0 183 1299
Size: 183 1116
BstBI TT'CG_AA
Cuts at: 0 598 1299
Size: 598 701
BstYI r'GATC_y
Cuts at: 0 9 200 754 910 1299
Size: 9 191 554 156 389
Fragments arranged by size:
554 389 191 156 9
BtgI C'CryG_G
Cuts at: 0 915 1299
Size: 915 384
BtsI GCAGTG_nn'
Cuts at: 0 288 746 1299
Size: 288 458 553
Fragments arranged by size:
553 458 288
EaeI y'GGCC_r
Cuts at: 0 285 1299
Size: 285 1014
EarI CTCTTCn'nnn_
Cuts at: 0 665 686 1299
Size: 665 21 613
Fragments arranged by size:
665 613 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 710 1113 1299
Size: 286 424 403 186
Fragments arranged by size:
424 403 286 186
EcoO109I rG'GnC_Cy
Cuts at: 0 88 1299
Size: 88 1211
EcoRV GAT'ATC
Cuts at: 0 1055 1299
Size: 1055 244
HindIII A'AGCT_T
Cuts at: 0 1292 1299
Size: 1292 7
MscI TGG'CCA
Cuts at: 0 287 1299
Size: 287 1012
MslI CAynn'nnrTG
Cuts at: 0 293 936 1004 1299
Size: 293 643 68 295
Fragments arranged by size:
643 295 293 68
NcoI C'CATG_G
Cuts at: 0 915 1299
Size: 915 384
NdeI CA'TA_TG
Cuts at: 0 168 1241 1299
Size: 168 1073 58
Fragments arranged by size:
1073 168 58
NspI r_CATG'y
Cuts at: 0 411 893 1109 1116 1193 1299
Size: 411 482 216 7 77 106
Fragments arranged by size:
482 411 216 106 77 7
PciI A'CATG_T
Cuts at: 0 889 1189 1299
Size: 889 300 110
Fragments arranged by size:
889 300 110
PfoI T'CCnGG_A
Cuts at: 0 263 665 1299
Size: 263 402 634
Fragments arranged by size:
634 402 263
PpuMI rG'GwC_Cy
Cuts at: 0 88 1299
Size: 88 1211
PspXI vC'TCGA_Gb
Cuts at: 0 608 1299
Size: 608 691
SmlI C'TyrA_G
Cuts at: 0 126 447 608 1299
Size: 126 321 161 691
Fragments arranged by size:
691 321 161 126
SspI AAT'ATT
Cuts at: 0 624 1299
Size: 624 675
StuI AGG'CCT
Cuts at: 0 687 1299
Size: 687 612
StyI C'CwwG_G
Cuts at: 0 84 682 789 915 953 1299
Size: 84 598 107 126 38 346
Fragments arranged by size:
598 346 126 107 84 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 315 347 1299
Size: 315 32 952
Fragments arranged by size:
952 315 32
XhoI C'TCGA_G
Cuts at: 0 608 1299
Size: 608 691
XmnI GAAnn'nnTTC
Cuts at: 0 1099 1299
Size: 1099 200
Enzymes that do cut:
AarI AcuI AflII AflIII AloI ApoI AvaI AvrII
BamHI BbsI BglII BmgBI BplI BpmI BpuEI BsaI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstAPI BstBI
BstYI BtgI BtsI EaeI EarI Eco57MI EcoO109I EcoRV
HindIII MscI MslI NcoI NdeI NspI PciI PfoI
PpuMI PspXI SmlI SspI StuI StyI TstI XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI BaeI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmrI BmtI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII TatI Tth111I XbaI
XcmI XmaI ZraI