Restriction map for
pFN21AB7704
(Linear) (Six-base) MAPSORT of: KIBB7704.seq Check: 118 from: 1 to: 1386
KIBB7704.orf
With 182 enzymes: SgfI *
July 3, 2012 16:28 ..
AccI GT'mk_AC
Cuts at: 0 104 1386
Size: 104 1282
Acc65I G'GTAC_C
Cuts at: 0 1156 1386
Size: 1156 230
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 839 872 1109 1386
Size: 17 822 33 237 277
Fragments arranged by size:
822 277 237 33 17
AflII C'TTAA_G
Cuts at: 0 676 1386
Size: 676 710
AhdI GACnn_n'nnGTC
Cuts at: 0 111 1386
Size: 111 1275
ApaLI G'TGCA_C
Cuts at: 0 546 1386
Size: 546 840
ApoI r'AATT_y
Cuts at: 0 95 1178 1386
Size: 95 1083 208
Fragments arranged by size:
1083 208 95
AvaI C'yCGr_G
Cuts at: 0 10 909 1386
Size: 10 899 477
Fragments arranged by size:
899 477 10
BanI G'GyrC_C
Cuts at: 0 160 410 1156 1386
Size: 160 250 746 230
Fragments arranged by size:
746 250 230 160
BbeI G_GCGC'C
Cuts at: 0 414 1386
Size: 414 972
BbsI GAAGACnn'nnnn_
Cuts at: 0 1064 1386
Size: 1064 322
BglI GCCn_nnn'nGGC
Cuts at: 0 408 1386
Size: 408 978
BlpI GC'TnA_GC
Cuts at: 0 452 1386
Size: 452 934
Bme1580I G_kGCm'C
Cuts at: 0 10 550 1386
Size: 10 540 836
Fragments arranged by size:
836 540 10
BmgBI CAC'GTC
Cuts at: 0 780 1386
Size: 780 606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 758 1324 1386
Size: 560 198 566 62
Fragments arranged by size:
566 560 198 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1264 1333 1386
Size: 1264 69 53
Fragments arranged by size:
1264 69 53
BsaHI Gr'CG_yC
Cuts at: 0 411 1386
Size: 411 975
BsaWI w'CCGG_w
Cuts at: 0 701 1386
Size: 701 685
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1084 1087 1386
Size: 1084 3 299
Fragments arranged by size:
1084 299 3
BsiEI CG_ry'CG
Cuts at: 0 108 1386
Size: 108 1278
BsiHKAI G_wGCw'C
Cuts at: 0 550 1386
Size: 550 836
Bsp1286I G_dGCh'C
Cuts at: 0 10 550 1386
Size: 10 540 836
Fragments arranged by size:
836 540 10
BspEI T'CCGG_A
Cuts at: 0 701 1386
Size: 701 685
BspHI T'CATG_A
Cuts at: 0 1256 1386
Size: 1256 130
BsrDI GCAATG_nn'
Cuts at: 0 143 845 1386
Size: 143 702 541
Fragments arranged by size:
702 541 143
BstYI r'GATC_y
Cuts at: 0 1248 1386
Size: 1248 138
BtgI C'CryG_G
Cuts at: 0 108 1386
Size: 108 1278
BtsI GCAGTG_nn'
Cuts at: 0 548 1386
Size: 548 838
EaeI y'GGCC_r
Cuts at: 0 1134 1386
Size: 1134 252
EarI CTCTTCn'nnn_
Cuts at: 0 1061 1085 1386
Size: 1061 24 301
Fragments arranged by size:
1061 301 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 560 758 839 872 1109 1324 1386
Size: 17 543 198 81 33 237 215 62
Fragments arranged by size:
543 237 215 198 81 62 33 17
EcoO109I rG'GnC_Cy
Cuts at: 0 228 720 1386
Size: 228 492 666
Fragments arranged by size:
666 492 228
EcoRV GAT'ATC
Cuts at: 0 564 905 1386
Size: 564 341 481
Fragments arranged by size:
564 481 341
HaeII r_GCGC'y
Cuts at: 0 140 212 414 1386
Size: 140 72 202 972
Fragments arranged by size:
972 202 140 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1386
Size: 97 32 1257
Fragments arranged by size:
1257 97 32
HincII GTy'rAC
Cuts at: 0 105 462 1386
Size: 105 357 924
Fragments arranged by size:
924 357 105
KasI G'GCGC_C
Cuts at: 0 410 1386
Size: 410 976
KpnI G_GTAC'C
Cuts at: 0 1160 1386
Size: 1160 226
MscI TGG'CCA
Cuts at: 0 1136 1386
Size: 1136 250
MslI CAynn'nnrTG
Cuts at: 0 1261 1386
Size: 1261 125
MspA1I CmG'CkG
Cuts at: 0 589 1110 1386
Size: 589 521 276
Fragments arranged by size:
589 521 276
NarI GG'CG_CC
Cuts at: 0 411 1386
Size: 411 975
NspI r_CATG'y
Cuts at: 0 331 1386
Size: 331 1055
PfoI T'CCnGG_A
Cuts at: 0 242 1386
Size: 242 1144
PpuMI rG'GwC_Cy
Cuts at: 0 720 1386
Size: 720 666
PsiI TTA'TAA
Cuts at: 0 515 1386
Size: 515 871
PstI C_TGCA'G
Cuts at: 0 1115 1386
Size: 1115 271
PvuII CAG'CTG
Cuts at: 0 589 1110 1386
Size: 589 521 276
Fragments arranged by size:
589 521 276
SalI G'TCGA_C
Cuts at: 0 103 1386
Size: 103 1283
SfcI C'TryA_G
Cuts at: 0 1111 1354 1386
Size: 1111 243 32
Fragments arranged by size:
1111 243 32
SfoI GGC'GCC
Cuts at: 0 412 1386
Size: 412 974
SmlI C'TyrA_G
Cuts at: 0 676 1279 1312 1386
Size: 676 603 33 74
Fragments arranged by size:
676 603 74 33
StyI C'CwwG_G
Cuts at: 0 863 1386
Size: 863 523
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 1386
Size: 178 1208
TatI w'GTAC_w
Cuts at: 0 334 761 1386
Size: 334 427 625
Fragments arranged by size:
625 427 334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 153 185 1386
Size: 153 32 1201
Fragments arranged by size:
1201 153 32
XbaI T'CTAG_A
Cuts at: 0 982 1386
Size: 982 404
XmnI GAAnn'nnTTC
Cuts at: 0 697 1386
Size: 697 689
Enzymes that do cut:
AccI Acc65I AcuI AflII AhdI ApaLI ApoI AvaI
BanI BbeI BbsI BglI BlpI Bme1580I BmgBI BpmI
BpuEI BsaHI BsaWI BseRI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BsrDI BstYI BtgI BtsI EaeI EarI Eco57MI
EcoO109I EcoRV HaeII Hin4I HincII KasI KpnI MscI
MslI MspA1I NarI NspI PfoI PpuMI PsiI PstI
PvuII SalI SfcI SfoI SmlI StyI TaqII TatI
TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbvCI BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaXI
BseYI BsgI BsiWI BsmI BsmBI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HindIII HpaI MfeI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XcmI XhoI XmaI
ZraI