Restriction map for
pFN21AB7686
(Linear) (Six-base) MAPSORT of: KIBB7686.seq Check: 96 from: 1 to: 1230
KIBB7686.orf
With 182 enzymes: SgfI *
March 22, 2012 15:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1027 1230
Size: 334 693 203
Fragments arranged by size:
693 334 203
AflIII A'CryG_T
Cuts at: 0 898 1230
Size: 898 332
AleI CACnn'nnGTG
Cuts at: 0 118 1230
Size: 118 1112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 282 1230
Size: 282 948
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 767 799 1230
Size: 767 32 431
Fragments arranged by size:
767 431 32
AlwNI CAG_nnn'CTG
Cuts at: 0 40 1230
Size: 40 1190
ApaI G_GGCC'C
Cuts at: 0 478 1230
Size: 478 752
AvaI C'yCGr_G
Cuts at: 0 787 841 1230
Size: 787 54 389
Fragments arranged by size:
787 389 54
BamHI G'GATC_C
Cuts at: 0 108 1011 1230
Size: 108 903 219
Fragments arranged by size:
903 219 108
BanI G'GyrC_C
Cuts at: 0 245 834 862 1230
Size: 245 589 28 368
Fragments arranged by size:
589 368 245 28
BanII G_rGCy'C
Cuts at: 0 31 127 478 504 1163 1230
Size: 31 96 351 26 659 67
Fragments arranged by size:
659 351 96 67 31 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 832 866 1230
Size: 832 34 364
Fragments arranged by size:
832 364 34
BglI GCCn_nnn'nGGC
Cuts at: 0 473 1230
Size: 473 757
BlpI GC'TnA_GC
Cuts at: 0 551 1057 1230
Size: 551 506 173
Fragments arranged by size:
551 506 173
Bme1580I G_kGCm'C
Cuts at: 0 271 478 867 1230
Size: 271 207 389 363
Fragments arranged by size:
389 363 271 207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 630 1230
Size: 630 600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 68 118 577 708 1230
Size: 54 14 50 459 131 522
Fragments arranged by size:
522 459 131 54 50 14
Bpu10I CC'TnA_GC
Cuts at: 0 1016 1230
Size: 1016 214
BsaI GGTCTCn'nnnn_
Cuts at: 0 658 809 1230
Size: 658 151 421
Fragments arranged by size:
658 421 151
BsaAI yAC'GTr
Cuts at: 0 1023 1230
Size: 1023 207
BsaHI Gr'CG_yC
Cuts at: 0 347 1230
Size: 347 883
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 767 797 1230
Size: 767 30 433
Fragments arranged by size:
767 433 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 231 606 618 775 1111 1153 1230
Size: 231 375 12 157 336 42 77
Fragments arranged by size:
375 336 231 157 77 42 12
BseYI C'CCAG_C
Cuts at: 0 58 1084 1230
Size: 58 1026 146
Fragments arranged by size:
1026 146 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1230
Size: 373 857
BsiEI CG_ry'CG
Cuts at: 0 427 1230
Size: 427 803
BsiHKAI G_wGCw'C
Cuts at: 0 31 1022 1163 1230
Size: 31 991 141 67
Fragments arranged by size:
991 141 67 31
BsiWI C'GTAC_G
Cuts at: 0 222 1230
Size: 222 1008
BsmBI CGTCTCn'nnnn_
Cuts at: 0 889 988 1230
Size: 889 99 242
Fragments arranged by size:
889 242 99
Bsp1286I G_dGCh'C
Cuts at: 0 31 127 271 478 504 867 1022 1163
Size: 31 96 144 207 26 363 155 141
Cuts at: 1163 1230
Size: 67
Fragments arranged by size:
363 207 155 144 141 96 67 31
26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 719 1230
Size: 719 511
BsrDI GCAATG_nn'
Cuts at: 0 603 1230
Size: 603 627
BsrFI r'CCGG_y
Cuts at: 0 308 856 872 1230
Size: 308 548 16 358
Fragments arranged by size:
548 358 308 16
BstAPI GCAn_nnn'nTGC
Cuts at: 0 197 296 1230
Size: 197 99 934
Fragments arranged by size:
934 197 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 92 504 1230
Size: 92 412 726
Fragments arranged by size:
726 412 92
BstYI r'GATC_y
Cuts at: 0 108 1011 1230
Size: 108 903 219
Fragments arranged by size:
903 219 108
BtgI C'CryG_G
Cuts at: 0 272 469 950 1230
Size: 272 197 481 280
Fragments arranged by size:
481 280 272 197
BtsI GCAGTG_nn'
Cuts at: 0 1142 1230
Size: 1142 88
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 939 974 1230
Size: 939 35 256
Fragments arranged by size:
939 256 35
EaeI y'GGCC_r
Cuts at: 0 424 521 689 884 1230
Size: 424 97 168 195 346
Fragments arranged by size:
424 346 195 168 97
EagI C'GGCC_G
Cuts at: 0 424 1230
Size: 424 806
EarI CTCTTCn'nnn_
Cuts at: 0 636 688 755 988 1230
Size: 636 52 67 233 242
Fragments arranged by size:
636 242 233 67 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 243 1230
Size: 243 987
EcoICRI GAG'CTC
Cuts at: 0 29 1161 1230
Size: 29 1132 69
Fragments arranged by size:
1132 69 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 68 118 334 577 708 1027 1230
Size: 54 14 50 216 243 131 319 203
Fragments arranged by size:
319 243 216 203 131 54 50 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 103 1230
Size: 103 1127
EcoO109I rG'GnC_Cy
Cuts at: 0 96 474 475 1002 1230
Size: 96 378 1 527 228
Fragments arranged by size:
527 378 228 96 1
HaeII r_GCGC'y
Cuts at: 0 366 1230
Size: 366 864
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 657 689 1230
Size: 657 32 541
Fragments arranged by size:
657 541 32
MluI A'CGCG_T
Cuts at: 0 898 1230
Size: 898 332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1230
Size: 434 796
MscI TGG'CCA
Cuts at: 0 523 886 1230
Size: 523 363 344
Fragments arranged by size:
523 363 344
MslI CAynn'nnrTG
Cuts at: 0 118 484 1230
Size: 118 366 746
Fragments arranged by size:
746 366 118
MspA1I CmG'CkG
Cuts at: 0 46 58 164 203 538 821 1230
Size: 46 12 106 39 335 283 409
Fragments arranged by size:
409 335 283 106 46 39 12
NaeI GCC'GGC
Cuts at: 0 310 874 1230
Size: 310 564 356
Fragments arranged by size:
564 356 310
NgoMIV G'CCGG_C
Cuts at: 0 308 872 1230
Size: 308 564 358
Fragments arranged by size:
564 358 308
PasI CC'CwG_GG
Cuts at: 0 504 867 1230
Size: 504 363 363
Fragments arranged by size:
504 363 363
PflMI CCAn_nnn'nTGG
Cuts at: 0 519 800 1230
Size: 519 281 430
Fragments arranged by size:
519 430 281
PfoI T'CCnGG_A
Cuts at: 0 70 737 1230
Size: 70 667 493
Fragments arranged by size:
667 493 70
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 767 799 1230
Size: 767 32 431
Fragments arranged by size:
767 431 32
PshAI GACnn'nnGTC
Cuts at: 0 821 901 1230
Size: 821 80 329
Fragments arranged by size:
821 329 80
PsiI TTA'TAA
Cuts at: 0 532 1230
Size: 532 698
PspOMI G'GGCC_C
Cuts at: 0 474 1230
Size: 474 756
PstI C_TGCA'G
Cuts at: 0 45 57 202 329 495 911 1230
Size: 45 12 145 127 166 416 319
Fragments arranged by size:
416 319 166 145 127 45 12
PvuII CAG'CTG
Cuts at: 0 46 58 538 821 1230
Size: 46 12 480 283 409
Fragments arranged by size:
480 409 283 46 12
SacI G_AGCT'C
Cuts at: 0 31 1163 1230
Size: 31 1132 67
Fragments arranged by size:
1132 67 31
SbfI CC_TGCA'GG
Cuts at: 0 329 911 1230
Size: 329 582 319
Fragments arranged by size:
582 329 319
SfcI C'TryA_G
Cuts at: 0 41 53 198 240 325 491 907 1230
Size: 41 12 145 42 85 166 416 323
Fragments arranged by size:
416 323 166 145 85 42 41 12
SspI AAT'ATT
Cuts at: 0 582 1230
Size: 582 648
StuI AGG'CCT
Cuts at: 0 304 847 973 1030 1230
Size: 304 543 126 57 200
Fragments arranged by size:
543 304 200 126 57
StyI C'CwwG_G
Cuts at: 0 974 1230
Size: 974 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 498 1230
Size: 498 732
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 751 783 1230
Size: 751 32 447
Fragments arranged by size:
751 447 32
XmnI GAAnn'nnTTC
Cuts at: 0 779 1230
Size: 779 451
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI AlwNI ApaI AvaI
BamHI BanI BanII BcgI BglI BlpI Bme1580I BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstXI BstYI BtgI BtsI CspCI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I MluI MmeI MscI MslI MspA1I NaeI NgoMIV
PasI PflMI PfoI PpiI PshAI PsiI PspOMI PstI
PvuII SacI SbfI SfcI SspI StuI StyI TaqII
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BpuEI BsaBI BsaWI BsmI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpuMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI ZraI