Restriction map for
pFN21AB7679
(Linear) (Six-base) MAPSORT of: KIBB7679.seq Check: 5845 from: 1 to: 1026
KIBB7679.orf
With 182 enzymes: SgfI *
March 22, 2012 15:39 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 1026
Size: 556 470
ApaI G_GGCC'C
Cuts at: 0 221 616 1026
Size: 221 395 410
Fragments arranged by size:
410 395 221
ApaLI G'TGCA_C
Cuts at: 0 605 1026
Size: 605 421
AvaI C'yCGr_G
Cuts at: 0 33 297 324 331 390 1026
Size: 33 264 27 7 59 636
Fragments arranged by size:
636 264 59 33 27 7
BanI G'GyrC_C
Cuts at: 0 60 1026
Size: 60 966
BanII G_rGCy'C
Cuts at: 0 221 399 616 995 1026
Size: 221 178 217 379 31
Fragments arranged by size:
379 221 217 178 31
BglI GCCn_nnn'nGGC
Cuts at: 0 235 454 1026
Size: 235 219 572
Fragments arranged by size:
572 235 219
Bme1580I G_kGCm'C
Cuts at: 0 65 221 554 609 616 1026
Size: 65 156 333 55 7 410
Fragments arranged by size:
410 333 156 65 55 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 619 837 1026
Size: 619 218 189
Fragments arranged by size:
619 218 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 618 1026
Size: 385 233 408
Fragments arranged by size:
408 385 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1026
Size: 151 875
BsaAI yAC'GTr
Cuts at: 0 429 1026
Size: 429 597
BsaHI Gr'CG_yC
Cuts at: 0 317 677 689 1026
Size: 317 360 12 337
Fragments arranged by size:
360 337 317 12
BsaWI w'CCGG_w
Cuts at: 0 310 1026
Size: 310 716
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 734 764 1026
Size: 112 30 592 30 262
Fragments arranged by size:
592 262 112 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 202 779 1026
Size: 199 3 577 247
Fragments arranged by size:
577 247 199 3
BseYI C'CCAG_C
Cuts at: 0 463 663 994 1026
Size: 463 200 331 32
Fragments arranged by size:
463 331 200 32
BsiEI CG_ry'CG
Cuts at: 0 245 275 347 420 1026
Size: 245 30 72 73 606
Fragments arranged by size:
606 245 73 72 30
BsiHKAI G_wGCw'C
Cuts at: 0 34 167 609 1026
Size: 34 133 442 417
Fragments arranged by size:
442 417 133 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 308 1013 1026
Size: 308 705 13
Fragments arranged by size:
705 308 13
Bsp1286I G_dGCh'C
Cuts at: 0 34 65 167 221 399 554 609 616
Size: 34 31 102 54 178 155 55 7
Cuts at: 616 995 1026
Size: 379 31
Fragments arranged by size:
379 178 155 102 55 54 34 31
31 7
BspEI T'CCGG_A
Cuts at: 0 310 1026
Size: 310 716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 797 1026
Size: 536 261 229
Fragments arranged by size:
536 261 229
BsrFI r'CCGG_y
Cuts at: 0 405 1026
Size: 405 621
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 1026
Size: 162 864
BtgI C'CryG_G
Cuts at: 0 48 370 452 1026
Size: 48 322 82 574
Fragments arranged by size:
574 322 82 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 472 1026
Size: 472 554
BtsI GCAGTG_nn'
Cuts at: 0 106 510 808 1026
Size: 106 404 298 218
Fragments arranged by size:
404 298 218 106
EaeI y'GGCC_r
Cuts at: 0 2 149 242 272 344 417 1026
Size: 2 147 93 30 72 73 609
Fragments arranged by size:
609 147 93 73 72 30 2
EagI C'GGCC_G
Cuts at: 0 242 272 344 417 1026
Size: 242 30 72 73 609
Fragments arranged by size:
609 242 73 72 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 274 505 755 1026
Size: 274 231 250 271
Fragments arranged by size:
274 271 250 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 618 1026
Size: 385 233 408
Fragments arranged by size:
408 385 233
EcoNI CCTnn'n_nnAGG
Cuts at: 0 700 1026
Size: 700 326
EcoO109I rG'GnC_Cy
Cuts at: 0 217 328 475 613 1026
Size: 217 111 147 138 413
Fragments arranged by size:
413 217 147 138 111
FspI TGC'GCA
Cuts at: 0 19 212 532 1026
Size: 19 193 320 494
Fragments arranged by size:
494 320 193 19
FspAI rTGC'GCAy
Cuts at: 0 19 1026
Size: 19 1007
HaeII r_GCGC'y
Cuts at: 0 138 1026
Size: 138 888
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 967 999 1026
Size: 967 32 27
Fragments arranged by size:
967 32 27
MspA1I CmG'CkG
Cuts at: 0 92 291 372 663 1026
Size: 92 199 81 291 363
Fragments arranged by size:
363 291 199 92 81
NcoI C'CATG_G
Cuts at: 0 48 1026
Size: 48 978
NotI GC'GGCC_GC
Cuts at: 0 242 344 417 1026
Size: 242 102 73 609
Fragments arranged by size:
609 242 102 73
NspI r_CATG'y
Cuts at: 0 642 718 1026
Size: 642 76 308
Fragments arranged by size:
642 308 76
PmlI CAC'GTG
Cuts at: 0 429 1026
Size: 429 597
PpuMI rG'GwC_Cy
Cuts at: 0 328 475 1026
Size: 328 147 551
Fragments arranged by size:
551 328 147
PspOMI G'GGCC_C
Cuts at: 0 217 612 1026
Size: 217 395 414
Fragments arranged by size:
414 395 217
PstI C_TGCA'G
Cuts at: 0 701 1026
Size: 701 325
RsrII CG'GwC_CG
Cuts at: 0 545 600 1026
Size: 545 55 426
Fragments arranged by size:
545 426 55
SacII CC_GC'GG
Cuts at: 0 373 1026
Size: 373 653
SanDI GG'GwC_CC
Cuts at: 0 328 475 1026
Size: 328 147 551
Fragments arranged by size:
551 328 147
SfcI C'TryA_G
Cuts at: 0 697 1026
Size: 697 329
SmaI CCC'GGG
Cuts at: 0 299 326 1026
Size: 299 27 700
Fragments arranged by size:
700 299 27
SmlI C'TyrA_G
Cuts at: 0 33 166 1026
Size: 33 133 860
Fragments arranged by size:
860 133 33
SphI G_CATG'C
Cuts at: 0 642 1026
Size: 642 384
StuI AGG'CCT
Cuts at: 0 229 1026
Size: 229 797
StyI C'CwwG_G
Cuts at: 0 48 735 1026
Size: 48 687 291
Fragments arranged by size:
687 291 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 734 766 914 946 1026
Size: 734 32 148 32 80
Fragments arranged by size:
734 148 80 32 32
XhoI C'TCGA_G
Cuts at: 0 33 1026
Size: 33 993
XmaI C'CCGG_G
Cuts at: 0 297 324 1026
Size: 297 27 702
Fragments arranged by size:
702 297 27
Enzymes that do cut:
AlfI ApaI ApaLI AvaI BanI BanII BglI Bme1580I
BmrI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI
BstAPI BtgI BtgZI BtsI EaeI EagI EciI Eco57MI
EcoNI EcoO109I FspI FspAI HaeII Hin4I MspA1I NcoI
NotI NspI PmlI PpuMI PspOMI PstI RsrII SacII
SanDI SfcI SmaI SmlI SphI StuI StyI TstI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaI BsaBI BsgI BsiWI BsmI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EarI EcoICRI EcoRI EcoRV FalI FseI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI
XmnI ZraI