Restriction map for
pFN21AB7673
(Linear) (Six-base) MAPSORT of: KIBB7673.seq Check: 8415 from: 1 to: 1020
KIBB7673.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 249 1020
Size: 249 771
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1020
Size: 719 301
AgeI A'CCGG_T
Cuts at: 0 7 1020
Size: 7 1013
AleI CACnn'nnGTG
Cuts at: 0 490 1020
Size: 490 530
ApaI G_GGCC'C
Cuts at: 0 115 424 1020
Size: 115 309 596
Fragments arranged by size:
596 309 115
AvaI C'yCGr_G
Cuts at: 0 108 123 219 256 417 1020
Size: 108 15 96 37 161 603
Fragments arranged by size:
603 161 108 96 37 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 770 803 1020
Size: 770 33 217
Fragments arranged by size:
770 217 33
BanI G'GyrC_C
Cuts at: 0 175 1020
Size: 175 845
BanII G_rGCy'C
Cuts at: 0 115 171 206 335 418 424 1020
Size: 115 56 35 129 83 6 596
Fragments arranged by size:
596 129 115 83 56 35 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 15 49 1020
Size: 15 34 971
Fragments arranged by size:
971 34 15
BglI GCCn_nnn'nGGC
Cuts at: 0 38 1020
Size: 38 982
BglII A'GATC_T
Cuts at: 0 819 1020
Size: 819 201
Bme1580I G_kGCm'C
Cuts at: 0 115 178 424 1020
Size: 115 63 246 596
Fragments arranged by size:
596 246 115 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 419 1020
Size: 419 601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1020
Size: 79 941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 1020
Size: 696 324
BsaI GGTCTCn'nnnn_
Cuts at: 0 929 1020
Size: 929 91
BsaHI Gr'CG_yC
Cuts at: 0 143 395 1020
Size: 143 252 625
Fragments arranged by size:
625 252 143
BsaWI w'CCGG_w
Cuts at: 0 7 17 1020
Size: 7 10 1003
Fragments arranged by size:
1003 10 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 394 397 1020
Size: 76 318 3 623
Fragments arranged by size:
623 318 76 3
BseYI C'CCAG_C
Cuts at: 0 148 336 368 1020
Size: 148 188 32 652
Fragments arranged by size:
652 188 148 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 1020
Size: 341 679
BsiEI CG_ry'CG
Cuts at: 0 8 188 278 322 1020
Size: 8 180 90 44 698
Fragments arranged by size:
698 180 90 44 8
BsiHKAI G_wGCw'C
Cuts at: 0 206 418 1020
Size: 206 212 602
Fragments arranged by size:
602 212 206
Bsp1286I G_dGCh'C
Cuts at: 0 115 171 178 206 335 418 424 1020
Size: 115 56 7 28 129 83 6 596
Fragments arranged by size:
596 129 115 83 56 28 7 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 249 1020
Size: 249 771
BsrBI CCG'CTC
Cuts at: 0 558 1020
Size: 558 462
BsrFI r'CCGG_y
Cuts at: 0 7 487 1020
Size: 7 480 533
Fragments arranged by size:
533 480 7
BssHII G'CGCG_C
Cuts at: 0 90 227 1020
Size: 90 137 793
Fragments arranged by size:
793 137 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 1020
Size: 492 528
BstYI r'GATC_y
Cuts at: 0 621 819 1020
Size: 621 198 201
Fragments arranged by size:
621 201 198
BtgI C'CryG_G
Cuts at: 0 161 313 1020
Size: 161 152 707
Fragments arranged by size:
707 161 152
EaeI y'GGCC_r
Cuts at: 0 185 1020
Size: 185 835
EagI C'GGCC_G
Cuts at: 0 185 1020
Size: 185 835
EarI CTCTTCn'nnn_
Cuts at: 0 451 1020
Size: 451 569
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 273 1020
Size: 273 747
EcoICRI GAG'CTC
Cuts at: 0 204 416 1020
Size: 204 212 604
Fragments arranged by size:
604 212 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 719 1020
Size: 79 640 301
Fragments arranged by size:
640 301 79
EcoO109I rG'GnC_Cy
Cuts at: 0 421 1020
Size: 421 599
HaeII r_GCGC'y
Cuts at: 0 483 1020
Size: 483 537
HincII GTy'rAC
Cuts at: 0 498 1020
Size: 498 522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1020
Size: 302 718
MslI CAynn'nnrTG
Cuts at: 0 490 1020
Size: 490 530
MspA1I CmG'CkG
Cuts at: 0 29 148 163 193 315 1011 1020
Size: 29 119 15 30 122 696 9
Fragments arranged by size:
696 122 119 30 29 15 9
NotI GC'GGCC_GC
Cuts at: 0 185 1020
Size: 185 835
PfoI T'CCnGG_A
Cuts at: 0 56 1020
Size: 56 964
PspOMI G'GGCC_C
Cuts at: 0 111 420 1020
Size: 111 309 600
Fragments arranged by size:
600 309 111
PvuII CAG'CTG
Cuts at: 0 1011 1020
Size: 1011 9
SacI G_AGCT'C
Cuts at: 0 206 418 1020
Size: 206 212 602
Fragments arranged by size:
602 212 206
SacII CC_GC'GG
Cuts at: 0 164 316 1020
Size: 164 152 704
Fragments arranged by size:
704 164 152
SfcI C'TryA_G
Cuts at: 0 643 1020
Size: 643 377
SgrAI Cr'CCGG_yG
Cuts at: 0 487 1020
Size: 487 533
SmaI CCC'GGG
Cuts at: 0 110 125 221 258 1020
Size: 110 15 96 37 762
Fragments arranged by size:
762 110 96 37 15
SmlI C'TyrA_G
Cuts at: 0 711 1020
Size: 711 309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 1020
Size: 230 32 758
Fragments arranged by size:
758 230 32
Tth111I GACn'n_nGTC
Cuts at: 0 394 1020
Size: 394 626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 1020
Size: 54 966
XmaI C'CCGG_G
Cuts at: 0 108 123 219 256 1020
Size: 108 15 96 37 764
Fragments arranged by size:
764 108 96 37 15
XmnI GAAnn'nnTTC
Cuts at: 0 663 1020
Size: 663 357
Enzymes that do cut:
AarI AcuI AgeI AleI ApaI AvaI BaeI BanI
BanII BcgI BglI BglII Bme1580I BmrI BpmI BpuEI
BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssHII BstXI BstYI BtgI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
HincII MmeI MslI MspA1I NotI PfoI PspOMI PvuII
SacI SacII SfcI SgrAI SmaI SmlI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI XbaI XhoI
ZraI