Restriction map for
pFN21AB7645
(Linear) (Six-base) MAPSORT of: KIBB7645.seq Check: 2773 from: 1 to: 984
KIBB7645.orf
With 182 enzymes: SgfI *
March 22, 2012 15:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 884 984
Size: 884 100
Acc65I G'GTAC_C
Cuts at: 0 845 984
Size: 845 139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 984
Size: 484 500
AfeI AGC'GCT
Cuts at: 0 675 984
Size: 675 309
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 595 627 984
Size: 595 32 357
Fragments arranged by size:
595 357 32
ApoI r'AATT_y
Cuts at: 0 422 984
Size: 422 562
AvaI C'yCGr_G
Cuts at: 0 87 108 792 804 858 984
Size: 87 21 684 12 54 126
Fragments arranged by size:
684 126 87 54 21 12
BamHI G'GATC_C
Cuts at: 0 862 984
Size: 862 122
BanI G'GyrC_C
Cuts at: 0 100 781 845 888 955 984
Size: 100 681 64 43 67 29
Fragments arranged by size:
681 100 67 64 43 29
BanII G_rGCy'C
Cuts at: 0 244 799 984
Size: 244 555 185
Fragments arranged by size:
555 244 185
BbeI G_GCGC'C
Cuts at: 0 104 785 892 959 984
Size: 104 681 107 67 25
Fragments arranged by size:
681 107 104 67 25
BglI GCCn_nnn'nGGC
Cuts at: 0 113 553 984
Size: 113 440 431
Fragments arranged by size:
440 431 113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 984
Size: 666 318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 42 462 783 984
Size: 26 16 420 321 201
Fragments arranged by size:
420 321 201 26 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 984
Size: 433 551
BsaAI yAC'GTr
Cuts at: 0 65 984
Size: 65 919
BsaHI Gr'CG_yC
Cuts at: 0 101 782 889 956 984
Size: 101 681 107 67 28
Fragments arranged by size:
681 107 101 67 28
BsaWI w'CCGG_w
Cuts at: 0 168 455 984
Size: 168 287 529
Fragments arranged by size:
529 287 168
BseYI C'CCAG_C
Cuts at: 0 381 984
Size: 381 603
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 984
Size: 883 101
Bsp1286I G_dGCh'C
Cuts at: 0 244 799 984
Size: 244 555 185
Fragments arranged by size:
555 244 185
BspEI T'CCGG_A
Cuts at: 0 455 984
Size: 455 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 884 984
Size: 884 100
BsrBI CCG'CTC
Cuts at: 0 92 984
Size: 92 892
BsrFI r'CCGG_y
Cuts at: 0 103 608 711 784 984
Size: 103 505 103 73 200
Fragments arranged by size:
505 200 103 103 73
BstYI r'GATC_y
Cuts at: 0 644 862 984
Size: 644 218 122
Fragments arranged by size:
644 218 122
BtgI C'CryG_G
Cuts at: 0 884 984
Size: 884 100
BtsI GCAGTG_nn'
Cuts at: 0 774 984
Size: 774 210
DraIII CAC_nnn'GTG
Cuts at: 0 688 984
Size: 688 296
EarI CTCTTCn'nnn_
Cuts at: 0 503 984
Size: 503 481
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 297 855 984
Size: 297 558 129
Fragments arranged by size:
558 297 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 42 462 484 783 984
Size: 26 16 420 22 299 201
Fragments arranged by size:
420 299 201 26 22 16
EcoO109I rG'GnC_Cy
Cuts at: 0 408 984
Size: 408 576
HaeII r_GCGC'y
Cuts at: 0 104 677 712 785 892 959 984
Size: 104 573 35 73 107 67 25
Fragments arranged by size:
573 107 104 73 67 35 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 984
Size: 22 32 930
Fragments arranged by size:
930 32 22
KasI G'GCGC_C
Cuts at: 0 100 781 888 955 984
Size: 100 681 107 67 29
Fragments arranged by size:
681 107 100 67 29
KpnI G_GTAC'C
Cuts at: 0 849 984
Size: 849 135
MspA1I CmG'CkG
Cuts at: 0 284 489 504 741 779 894 984
Size: 284 205 15 237 38 115 90
Fragments arranged by size:
284 237 205 115 90 38 15
NaeI GCC'GGC
Cuts at: 0 105 713 786 984
Size: 105 608 73 198
Fragments arranged by size:
608 198 105 73
NarI GG'CG_CC
Cuts at: 0 101 782 889 956 984
Size: 101 681 107 67 28
Fragments arranged by size:
681 107 101 67 28
NgoMIV G'CCGG_C
Cuts at: 0 103 711 784 984
Size: 103 608 73 200
Fragments arranged by size:
608 200 103 73
PasI CC'CwG_GG
Cuts at: 0 664 984
Size: 664 320
PpuMI rG'GwC_Cy
Cuts at: 0 408 984
Size: 408 576
PshAI GACnn'nnGTC
Cuts at: 0 7 583 984
Size: 7 576 401
Fragments arranged by size:
576 401 7
PstI C_TGCA'G
Cuts at: 0 881 984
Size: 881 103
PvuII CAG'CTG
Cuts at: 0 489 984
Size: 489 495
SapI GCTCTTCn'nnn_
Cuts at: 0 503 984
Size: 503 481
SbfI CC_TGCA'GG
Cuts at: 0 881 984
Size: 881 103
SfcI C'TryA_G
Cuts at: 0 877 984
Size: 877 107
SfoI GGC'GCC
Cuts at: 0 102 783 890 957 984
Size: 102 681 107 67 27
Fragments arranged by size:
681 107 102 67 27
SmaI CCC'GGG
Cuts at: 0 110 984
Size: 110 874
SmlI C'TyrA_G
Cuts at: 0 412 984
Size: 412 572
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 984
Size: 589 395
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 624 656 984
Size: 247 32 345 32 328
Fragments arranged by size:
345 328 247 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 486 984
Size: 486 498
XmaI C'CCGG_G
Cuts at: 0 108 984
Size: 108 876
XmnI GAAnn'nnTTC
Cuts at: 0 372 984
Size: 372 612
Enzymes that do cut:
AarI Acc65I AcuI AfeI AloI ApoI AvaI BamHI
BanI BanII BbeI BglI BplI BpmI BpuEI BsaAI
BsaHI BsaWI BseYI BsgI Bsp1286I BspEI BspMI BsrBI
BsrFI BstYI BtgI BtsI DraIII EarI EciI Eco57MI
EcoO109I HaeII Hin4I KasI KpnI MspA1I NaeI NarI
NgoMIV PasI PpuMI PshAI PstI PvuII SapI SbfI
SfcI SfoI SmaI SmlI TaqII TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BsaI BsaBI
BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI MmeI MscI
MslI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI Tth111I
XbaI XhoI ZraI