Restriction map for
pFN21AB7634
(Linear) (Six-base) MAPSORT of: KIBB7634.seq Check: 3206 from: 1 to: 1125
KIBB7634.orf
With 182 enzymes: SgfI *
July 3, 2012 16:26 ..
AclI AA'CG_TT
Cuts at: 0 1062 1125
Size: 1062 63
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 383 670 1125
Size: 275 108 287 455
Fragments arranged by size:
455 287 275 108
AhdI GACnn_n'nnGTC
Cuts at: 0 531 1125
Size: 531 594
AleI CACnn'nnGTG
Cuts at: 0 479 1125
Size: 479 646
ApoI r'AATT_y
Cuts at: 0 369 456 872 1125
Size: 369 87 416 253
Fragments arranged by size:
416 369 253 87
BamHI G'GATC_C
Cuts at: 0 719 1125
Size: 719 406
BanI G'GyrC_C
Cuts at: 0 1051 1125
Size: 1051 74
BanII G_rGCy'C
Cuts at: 0 125 731 1125
Size: 125 606 394
Fragments arranged by size:
606 394 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 227 261 1125
Size: 227 34 864
Fragments arranged by size:
864 227 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 225 1125
Size: 225 900
BfrBI ATG'CAT
Cuts at: 0 220 1125
Size: 220 905
BglII A'GATC_T
Cuts at: 0 1070 1125
Size: 1070 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 181 1125
Size: 181 944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1125
Size: 139 986
Bpu10I CC'TnA_GC
Cuts at: 0 146 1125
Size: 146 979
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 176 1125
Size: 103 73 949
Fragments arranged by size:
949 103 73
BsaWI w'CCGG_w
Cuts at: 0 228 1125
Size: 228 897
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 511 541 1125
Size: 511 30 584
Fragments arranged by size:
584 511 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 1125
Size: 121 1004
BseYI C'CCAG_C
Cuts at: 0 409 429 1125
Size: 409 20 696
Fragments arranged by size:
696 409 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 756 853 1125
Size: 457 299 97 272
Fragments arranged by size:
457 299 272 97
BsiHKAI G_wGCw'C
Cuts at: 0 125 1125
Size: 125 1000
BsmI GAATG_Cn'
Cuts at: 0 222 1125
Size: 222 903
Bsp1286I G_dGCh'C
Cuts at: 0 125 731 1125
Size: 125 606 394
Fragments arranged by size:
606 394 125
BspEI T'CCGG_A
Cuts at: 0 228 1125
Size: 228 897
BsrBI CCG'CTC
Cuts at: 0 24 145 1125
Size: 24 121 980
Fragments arranged by size:
980 121 24
BsrGI T'GTAC_A
Cuts at: 0 206 1125
Size: 206 919
BssSI C'ACGA_G
Cuts at: 0 824 1125
Size: 824 301
BstAPI GCAn_nnn'nTGC
Cuts at: 0 676 1125
Size: 676 449
BstBI TT'CG_AA
Cuts at: 0 247 1125
Size: 247 878
BstXI CCAn_nnnn'nTGG
Cuts at: 0 918 1125
Size: 918 207
BstYI r'GATC_y
Cuts at: 0 291 645 719 1070 1125
Size: 291 354 74 351 55
Fragments arranged by size:
354 351 291 74 55
BtgI C'CryG_G
Cuts at: 0 95 1125
Size: 95 1030
DraI TTT'AAA
Cuts at: 0 870 1116 1125
Size: 870 246 9
Fragments arranged by size:
870 246 9
DrdI GACnn_nn'nnGTC
Cuts at: 0 340 1125
Size: 340 785
EaeI y'GGCC_r
Cuts at: 0 16 1125
Size: 16 1109
EarI CTCTTCn'nnn_
Cuts at: 0 785 1125
Size: 785 340
EcoICRI GAG'CTC
Cuts at: 0 123 1125
Size: 123 1002
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 275 383 670 1125
Size: 139 136 108 287 455
Fragments arranged by size:
455 287 139 136 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 475 507 517 549 896 928
Size: 268 32 175 32 10 32 347 32
Cuts at: 928 1125
Size: 197
Fragments arranged by size:
347 268 197 175 32 32 32 32
10
HincII GTy'rAC
Cuts at: 0 1046 1125
Size: 1046 79
HindIII A'AGCT_T
Cuts at: 0 838 1125
Size: 838 287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 623 867 1125
Size: 548 75 244 258
Fragments arranged by size:
548 258 244 75
MslI CAynn'nnrTG
Cuts at: 0 479 1125
Size: 479 646
MspA1I CmG'CkG
Cuts at: 0 471 1125
Size: 471 654
NcoI C'CATG_G
Cuts at: 0 95 1125
Size: 95 1030
NsiI A_TGCA'T
Cuts at: 0 222 1125
Size: 222 903
NspI r_CATG'y
Cuts at: 0 670 1125
Size: 670 455
PvuII CAG'CTG
Cuts at: 0 471 1125
Size: 471 654
SacI G_AGCT'C
Cuts at: 0 125 1125
Size: 125 1000
SapI GCTCTTCn'nnn_
Cuts at: 0 785 1125
Size: 785 340
SfcI C'TryA_G
Cuts at: 0 604 1125
Size: 604 521
SphI G_CATG'C
Cuts at: 0 670 1125
Size: 670 455
StyI C'CwwG_G
Cuts at: 0 95 1125
Size: 95 1030
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 180 423 1125
Size: 180 243 702
Fragments arranged by size:
702 243 180
TatI w'GTAC_w
Cuts at: 0 206 1125
Size: 206 919
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1025 1057 1125
Size: 1025 32 68
Fragments arranged by size:
1025 68 32
Tth111I GACn'n_nGTC
Cuts at: 0 493 1125
Size: 493 632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 627 1125
Size: 627 498
XmnI GAAnn'nnTTC
Cuts at: 0 971 1125
Size: 971 154
Enzymes that do cut:
AclI AcuI AhdI AleI ApoI BamHI BanI BanII
BcgI BciVI BfrBI BglII BmrI BpmI Bpu10I BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BsrBI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI DraI DrdI EaeI EarI EcoICRI Eco57MI Hin4I
HincII HindIII MmeI MslI MspA1I NcoI NsiI NspI
PvuII SacI SapI SfcI SphI StyI TaqII TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BbvCI BclI BglI
BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BspHI BspMI BsrDI BsrFI
BssHII BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI