Restriction map for
pFN21AB7616
(Linear) (Six-base) MAPSORT of: KIBB7616.seq Check: 9811 from: 1 to: 1083
KIBB7616.orf
With 182 enzymes: SgfI *
March 22, 2012 15:38 ..
AatII G_ACGT'C
Cuts at: 0 524 1083
Size: 524 559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 1083
Size: 395 688
AfeI AGC'GCT
Cuts at: 0 23 155 1083
Size: 23 132 928
Fragments arranged by size:
928 132 23
AleI CACnn'nnGTG
Cuts at: 0 955 1083
Size: 955 128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 937 973 1083
Size: 937 36 110
Fragments arranged by size:
937 110 36
AlwNI CAG_nnn'CTG
Cuts at: 0 828 1083
Size: 828 255
ApaI G_GGCC'C
Cuts at: 0 242 868 1083
Size: 242 626 215
Fragments arranged by size:
626 242 215
ApaLI G'TGCA_C
Cuts at: 0 84 1083
Size: 84 999
AvaI C'yCGr_G
Cuts at: 0 63 132 172 241 530 697 808 1083
Size: 63 69 40 69 289 167 111 275
Fragments arranged by size:
289 275 167 111 69 69 63 40
BanI G'GyrC_C
Cuts at: 0 70 1083
Size: 70 1013
BanII G_rGCy'C
Cuts at: 0 131 139 242 251 662 868 1083
Size: 131 8 103 9 411 206 215
Fragments arranged by size:
411 215 206 131 103 9 8
BbeI G_GCGC'C
Cuts at: 0 74 1083
Size: 74 1009
BbsI GAAGACnn'nnnn_
Cuts at: 0 572 1040 1083
Size: 572 468 43
Fragments arranged by size:
572 468 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 75 109 776 810 1083
Size: 75 34 667 34 273
Fragments arranged by size:
667 273 75 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 566 1083
Size: 566 517
BglI GCCn_nnn'nGGC
Cuts at: 0 246 1083
Size: 246 837
BlpI GC'TnA_GC
Cuts at: 0 25 202 1083
Size: 25 177 881
Fragments arranged by size:
881 177 25
Bme1580I G_kGCm'C
Cuts at: 0 88 179 242 747 868 1083
Size: 88 91 63 505 121 215
Fragments arranged by size:
505 215 121 91 88 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 98 766 1083
Size: 98 668 317
Fragments arranged by size:
668 317 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 508 799 1083
Size: 372 136 291 284
Fragments arranged by size:
372 291 284 136
Bpu10I CC'TnA_GC
Cuts at: 0 875 938 1083
Size: 875 63 145
Fragments arranged by size:
875 145 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1083
Size: 694 389
BsaHI Gr'CG_yC
Cuts at: 0 71 521 1083
Size: 71 450 562
Fragments arranged by size:
562 450 71
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 496 1027 1030 1033 1083
Size: 496 531 3 3 50
Fragments arranged by size:
531 496 50 3 3
BseYI C'CCAG_C
Cuts at: 0 626 960 1083
Size: 626 334 123
Fragments arranged by size:
626 334 123
BsiEI CG_ry'CG
Cuts at: 0 396 1083
Size: 396 687
BsiHKAI G_wGCw'C
Cuts at: 0 88 139 203 926 947 1083
Size: 88 51 64 723 21 136
Fragments arranged by size:
723 136 88 64 51 21
BsmI GAATG_Cn'
Cuts at: 0 496 1083
Size: 496 587
Bsp1286I G_dGCh'C
Cuts at: 0 88 131 139 179 203 242 251 662
Size: 88 43 8 40 24 39 9 411
Cuts at: 662 747 868 926 947 1083
Size: 85 121 58 21 136
Fragments arranged by size:
411 136 121 88 85 58 43 40
39 24 21 9 8
BsrBI CCG'CTC
Cuts at: 0 861 1083
Size: 861 222
BsrDI GCAATG_nn'
Cuts at: 0 914 1083
Size: 914 169
BsrFI r'CCGG_y
Cuts at: 0 1041 1083
Size: 1041 42
BssHII G'CGCG_C
Cuts at: 0 190 1083
Size: 190 893
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 960 1083
Size: 279 681 123
Fragments arranged by size:
681 279 123
BstYI r'GATC_y
Cuts at: 0 107 1083
Size: 107 976
BtgI C'CryG_G
Cuts at: 0 145 209 671 748 1025 1083
Size: 145 64 462 77 277 58
Fragments arranged by size:
462 277 145 77 64 58
BtsI GCAGTG_nn'
Cuts at: 0 645 1083
Size: 645 438
DraI TTT'AAA
Cuts at: 0 419 1083
Size: 419 664
EaeI y'GGCC_r
Cuts at: 0 50 77 359 542 881 1083
Size: 50 27 282 183 339 202
Fragments arranged by size:
339 282 202 183 50 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 664 1060 1083
Size: 664 396 23
Fragments arranged by size:
664 396 23
EcoICRI GAG'CTC
Cuts at: 0 137 1083
Size: 137 946
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 395 508 799 1083
Size: 372 23 113 291 284
Fragments arranged by size:
372 291 284 113 23
EcoO109I rG'GnC_Cy
Cuts at: 0 238 825 864 865 1083
Size: 238 587 39 1 218
Fragments arranged by size:
587 238 218 39 1
HaeII r_GCGC'y
Cuts at: 0 25 74 157 1083
Size: 25 49 83 926
Fragments arranged by size:
926 83 49 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 1041 1073 1083
Size: 370 32 639 32 10
Fragments arranged by size:
639 370 32 32 10
KasI G'GCGC_C
Cuts at: 0 70 1083
Size: 70 1013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1083
Size: 317 766
MscI TGG'CCA
Cuts at: 0 52 544 1083
Size: 52 492 539
Fragments arranged by size:
539 492 52
MslI CAynn'nnrTG
Cuts at: 0 919 955 1083
Size: 919 36 128
Fragments arranged by size:
919 128 36
MspA1I CmG'CkG
Cuts at: 0 147 211 342 1068 1083
Size: 147 64 131 726 15
Fragments arranged by size:
726 147 131 64 15
NaeI GCC'GGC
Cuts at: 0 1043 1083
Size: 1043 40
NarI GG'CG_CC
Cuts at: 0 71 1083
Size: 71 1012
NcoI C'CATG_G
Cuts at: 0 671 1025 1083
Size: 671 354 58
Fragments arranged by size:
671 354 58
NgoMIV G'CCGG_C
Cuts at: 0 1041 1083
Size: 1041 42
PfoI T'CCnGG_A
Cuts at: 0 101 278 1083
Size: 101 177 805
Fragments arranged by size:
805 177 101
PspOMI G'GGCC_C
Cuts at: 0 238 864 1083
Size: 238 626 219
Fragments arranged by size:
626 238 219
PvuI CG_AT'CG
Cuts at: 0 396 1083
Size: 396 687
PvuII CAG'CTG
Cuts at: 0 1068 1083
Size: 1068 15
SacI G_AGCT'C
Cuts at: 0 139 1083
Size: 139 944
SacII CC_GC'GG
Cuts at: 0 148 212 1083
Size: 148 64 871
Fragments arranged by size:
871 148 64
SfoI GGC'GCC
Cuts at: 0 72 1083
Size: 72 1011
SmaI CCC'GGG
Cuts at: 0 174 243 532 1083
Size: 174 69 289 551
Fragments arranged by size:
551 289 174 69
SmlI C'TyrA_G
Cuts at: 0 709 1083
Size: 709 374
StyI C'CwwG_G
Cuts at: 0 671 719 1025 1083
Size: 671 48 306 58
Fragments arranged by size:
671 306 58 48
Tth111I GACn'n_nGTC
Cuts at: 0 969 1083
Size: 969 114
XmaI C'CCGG_G
Cuts at: 0 172 241 530 1083
Size: 172 69 289 553
Fragments arranged by size:
553 289 172 69
XmnI GAAnn'nnTTC
Cuts at: 0 496 1083
Size: 496 587
ZraI GAC'GTC
Cuts at: 0 522 1083
Size: 522 561
Enzymes that do cut:
AatII AcuI AfeI AleI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BcgI BciVI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI
BssHII BstXI BstYI BtgI BtsI DraI EaeI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PfoI PspOMI
PvuI PvuII SacI SacII SfoI SmaI SmlI StyI
Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BclI BfrBI BglII BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmBI BspEI
BspHI BspMI BsrGI BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EarI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XcmI XhoI