Restriction map for
pFN21AB7609
(Linear) (Six-base) MAPSORT of: KIBB7609.seq Check: 1641 from: 1 to: 900
KIBB7609.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 693 900
Size: 352 341 207
Fragments arranged by size:
352 341 207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 900
Size: 283 617
AlwNI CAG_nnn'CTG
Cuts at: 0 330 900
Size: 330 570
ApoI r'AATT_y
Cuts at: 0 131 631 821 859 900
Size: 131 500 190 38 41
Fragments arranged by size:
500 190 131 41 38
AseI AT'TA_AT
Cuts at: 0 676 900
Size: 676 224
AvaI C'yCGr_G
Cuts at: 0 281 609 875 900
Size: 281 328 266 25
Fragments arranged by size:
328 281 266 25
BanII G_rGCy'C
Cuts at: 0 507 900
Size: 507 393
BbsI GAAGACnn'nnnn_
Cuts at: 0 695 699 852 900
Size: 695 4 153 48
Fragments arranged by size:
695 153 48 4
BciVI GTATCCnnnnn_n'
Cuts at: 0 226 900
Size: 226 674
BglII A'GATC_T
Cuts at: 0 509 900
Size: 509 391
BlpI GC'TnA_GC
Cuts at: 0 737 787 900
Size: 737 50 113
Fragments arranged by size:
737 113 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 635 900
Size: 635 265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 847 900
Size: 160 687 53
Fragments arranged by size:
687 160 53
BsaAI yAC'GTr
Cuts at: 0 89 900
Size: 89 811
BsaHI Gr'CG_yC
Cuts at: 0 704 900
Size: 704 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 35 67 97 900
Size: 5 30 32 30 803
Fragments arranged by size:
803 32 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 900
Size: 298 602
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 900
Size: 369 531
BsiHKAI G_wGCw'C
Cuts at: 0 251 900
Size: 251 649
BsmI GAATG_Cn'
Cuts at: 0 876 900
Size: 876 24
Bsp1286I G_dGCh'C
Cuts at: 0 251 507 900
Size: 251 256 393
Fragments arranged by size:
393 256 251
BspHI T'CATG_A
Cuts at: 0 416 900
Size: 416 484
BsrDI GCAATG_nn'
Cuts at: 0 99 900
Size: 99 801
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 900
Size: 201 699
BstYI r'GATC_y
Cuts at: 0 509 900
Size: 509 391
BtsI GCAGTG_nn'
Cuts at: 0 737 900
Size: 737 163
EaeI y'GGCC_r
Cuts at: 0 342 900
Size: 342 558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 352 693 847 900
Size: 160 192 341 154 53
Fragments arranged by size:
341 192 160 154 53
EcoRI G'AATT_C
Cuts at: 0 631 900
Size: 631 269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 900
Size: 67 32 801
Fragments arranged by size:
801 67 32
MfeI C'AATT_G
Cuts at: 0 210 900
Size: 210 690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 900
Size: 461 439
MspA1I CmG'CkG
Cuts at: 0 201 900
Size: 201 699
PfoI T'CCnGG_A
Cuts at: 0 185 900
Size: 185 715
PmlI CAC'GTG
Cuts at: 0 89 900
Size: 89 811
PstI C_TGCA'G
Cuts at: 0 259 900
Size: 259 641
PvuII CAG'CTG
Cuts at: 0 201 900
Size: 201 699
ScaI AGT'ACT
Cuts at: 0 145 900
Size: 145 755
SexAI A'CCwGG_T
Cuts at: 0 158 900
Size: 158 742
SfcI C'TryA_G
Cuts at: 0 255 312 900
Size: 255 57 588
Fragments arranged by size:
588 255 57
SmaI CCC'GGG
Cuts at: 0 283 900
Size: 283 617
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 707 900
Size: 707 193
TatI w'GTAC_w
Cuts at: 0 143 900
Size: 143 757
XmaI C'CCGG_G
Cuts at: 0 281 900
Size: 281 619
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AseI AvaI BanII BbsI
BciVI BglII BlpI BplI BpmI BsaAI BsaHI BsaXI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstXI
BstYI BtsI EaeI Eco57MI EcoRI Hin4I MfeI MmeI
MspA1I PfoI PmlI PstI PvuII ScaI SexAI SfcI
SmaI TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI
BfrBI BglI Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaBI BsaWI BseYI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI