Restriction map for
pFN21AB7606
(Linear) (Six-base) MAPSORT of: KIBB7606.seq Check: 9647 from: 1 to: 1068
KIBB7606.orf
With 182 enzymes: SgfI *
March 22, 2012 15:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5 1068
Size: 5 1063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1068
Size: 694 374
AflIII A'CryG_T
Cuts at: 0 798 846 1068
Size: 798 48 222
Fragments arranged by size:
798 222 48
AlwNI CAG_nnn'CTG
Cuts at: 0 9 1068
Size: 9 1059
AvaI C'yCGr_G
Cuts at: 0 112 189 1068
Size: 112 77 879
Fragments arranged by size:
879 112 77
AvrII C'CTAG_G
Cuts at: 0 324 1068
Size: 324 744
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 796 829 1068
Size: 796 33 239
Fragments arranged by size:
796 239 33
BanI G'GyrC_C
Cuts at: 0 85 631 1068
Size: 85 546 437
Fragments arranged by size:
546 437 85
BanII G_rGCy'C
Cuts at: 0 29 112 196 684 1068
Size: 29 83 84 488 384
Fragments arranged by size:
488 384 84 83 29
BbeI G_GCGC'C
Cuts at: 0 89 1068
Size: 89 979
BglI GCCn_nnn'nGGC
Cuts at: 0 64 209 1068
Size: 64 145 859
Fragments arranged by size:
859 145 64
Bme1580I G_kGCm'C
Cuts at: 0 636 950 1068
Size: 636 314 118
Fragments arranged by size:
636 314 118
BmgBI CAC'GTC
Cuts at: 0 799 849 1068
Size: 799 50 219
Fragments arranged by size:
799 219 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 450 871 1068
Size: 450 421 197
Fragments arranged by size:
450 421 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 748 882 1003 1068
Size: 259 489 134 121 65
Fragments arranged by size:
489 259 134 121 65
Bpu10I CC'TnA_GC
Cuts at: 0 734 1068
Size: 734 334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 810 1068
Size: 247 563 258
Fragments arranged by size:
563 258 247
BsaAI yAC'GTr
Cuts at: 0 945 1068
Size: 945 123
BsaHI Gr'CG_yC
Cuts at: 0 86 1068
Size: 86 982
BsaWI w'CCGG_w
Cuts at: 0 290 446 1068
Size: 290 156 622
Fragments arranged by size:
622 290 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 746 776 853 883 1068
Size: 124 30 592 30 77 30 185
Fragments arranged by size:
592 185 124 77 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 132 135 538 628 1068
Size: 52 80 3 403 90 440
Fragments arranged by size:
440 403 90 80 52 3
BsiEI CG_ry'CG
Cuts at: 0 68 1068
Size: 68 1000
BsiHKAI G_wGCw'C
Cuts at: 0 142 684 834 1068
Size: 142 542 150 234
Fragments arranged by size:
542 234 150 142
Bsp1286I G_dGCh'C
Cuts at: 0 29 112 142 196 636 684 834 950
Size: 29 83 30 54 440 48 150 116
Cuts at: 950 1068
Size: 118
Fragments arranged by size:
440 150 118 116 83 54 48 30
29
BspEI T'CCGG_A
Cuts at: 0 446 1068
Size: 446 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 5 1068
Size: 5 1063
BsrBI CCG'CTC
Cuts at: 0 121 1068
Size: 121 947
BssSI C'ACGA_G
Cuts at: 0 135 1068
Size: 135 933
BstAPI GCAn_nnn'nTGC
Cuts at: 0 230 311 484 730 1068
Size: 230 81 173 246 338
Fragments arranged by size:
338 246 230 173 81
BtgI C'CryG_G
Cuts at: 0 182 1068
Size: 182 886
BtsI GCAGTG_nn'
Cuts at: 0 312 1068
Size: 312 756
DraI TTT'AAA
Cuts at: 0 590 1068
Size: 590 478
EaeI y'GGCC_r
Cuts at: 0 65 1068
Size: 65 1003
EagI C'GGCC_G
Cuts at: 0 65 1068
Size: 65 1003
EarI CTCTTCn'nnn_
Cuts at: 0 89 536 1068
Size: 89 447 532
Fragments arranged by size:
532 447 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 63 1068
Size: 63 1005
EcoICRI GAG'CTC
Cuts at: 0 682 1068
Size: 682 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 694 748 882 1003 1068
Size: 259 435 54 134 121 65
Fragments arranged by size:
435 259 134 121 65 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 501 1068
Size: 262 239 567
Fragments arranged by size:
567 262 239
HaeII r_GCGC'y
Cuts at: 0 89 1068
Size: 89 979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 118 124 150 156 1068
Size: 3 32 83 6 26 6 912
Fragments arranged by size:
912 83 32 26 6 6 3
HincII GTy'rAC
Cuts at: 0 657 968 1068
Size: 657 311 100
Fragments arranged by size:
657 311 100
HpaI GTT'AAC
Cuts at: 0 657 968 1068
Size: 657 311 100
Fragments arranged by size:
657 311 100
KasI G'GCGC_C
Cuts at: 0 85 1068
Size: 85 983
MspA1I CmG'CkG
Cuts at: 0 156 341 1068
Size: 156 185 727
Fragments arranged by size:
727 185 156
NarI GG'CG_CC
Cuts at: 0 86 1068
Size: 86 982
NcoI C'CATG_G
Cuts at: 0 182 1068
Size: 182 886
NspI r_CATG'y
Cuts at: 0 289 482 579 1068
Size: 289 193 97 489
Fragments arranged by size:
489 289 193 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 1068
Size: 771 297
PstI C_TGCA'G
Cuts at: 0 307 559 1068
Size: 307 252 509
Fragments arranged by size:
509 307 252
PvuII CAG'CTG
Cuts at: 0 341 1068
Size: 341 727
SacI G_AGCT'C
Cuts at: 0 684 1068
Size: 684 384
SfcI C'TryA_G
Cuts at: 0 53 303 555 1068
Size: 53 250 252 513
Fragments arranged by size:
513 252 250 53
SfoI GGC'GCC
Cuts at: 0 87 1068
Size: 87 981
SmlI C'TyrA_G
Cuts at: 0 262 789 1068
Size: 262 527 279
Fragments arranged by size:
527 279 262
SphI G_CATG'C
Cuts at: 0 482 579 1068
Size: 482 97 489
Fragments arranged by size:
489 482 97
StyI C'CwwG_G
Cuts at: 0 182 324 455 1068
Size: 182 142 131 613
Fragments arranged by size:
613 182 142 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 927 1068
Size: 927 141
TatI w'GTAC_w
Cuts at: 0 561 1068
Size: 561 507
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 746 778 853 885 1068
Size: 746 32 75 32 183
Fragments arranged by size:
746 183 75 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 442 1068
Size: 442 626
Enzymes that do cut:
AarI AcuI AflIII AlwNI AvaI AvrII BaeI BanI
BanII BbeI BglI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BssSI BstAPI BtgI BtsI
DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
HaeII Hin4I HincII HpaI KasI MspA1I NarI NcoI
NspI PflMI PstI PvuII SacI SfcI SfoI SmlI
SphI StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BplI BsaI BsaBI BseYI BsgI
BsiWI BsmI BsmBI BspHI BsrDI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HindIII KpnI MfeI MluI MmeI MscI
MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
XbaI XcmI XhoI XmaI XmnI ZraI