Restriction map for
pFN21AB7596
(Linear) (Six-base) MAPSORT of: KIBB7596.seq Check: 1862 from: 1 to: 876
KIBB7596.orf
With 182 enzymes: SgfI *
March 22, 2012 15:37 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 77 876
Size: 77 799
AlwNI CAG_nnn'CTG
Cuts at: 0 377 876
Size: 377 499
ApaI G_GGCC'C
Cuts at: 0 459 876
Size: 459 417
BanII G_rGCy'C
Cuts at: 0 14 152 386 459 876
Size: 14 138 234 73 417
Fragments arranged by size:
417 234 138 73 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 422 876
Size: 422 454
BbvCI CC'TCA_GC
Cuts at: 0 404 876
Size: 404 472
BciVI GTATCCnnnnn_n'
Cuts at: 0 197 835 876
Size: 197 638 41
Fragments arranged by size:
638 197 41
BglII A'GATC_T
Cuts at: 0 593 876
Size: 593 283
Bme1580I G_kGCm'C
Cuts at: 0 459 876
Size: 459 417
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 876
Size: 315 561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 312 448 876
Size: 223 89 136 428
Fragments arranged by size:
428 223 136 89
Bpu10I CC'TnA_GC
Cuts at: 0 45 404 876
Size: 45 359 472
Fragments arranged by size:
472 359 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 213 344 876
Size: 213 131 532
Fragments arranged by size:
532 213 131
BsaWI w'CCGG_w
Cuts at: 0 629 876
Size: 629 247
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 243 409 412 414 778 802 876
Size: 243 166 3 2 364 24 74
Fragments arranged by size:
364 243 166 74 24 3 2
BseYI C'CCAG_C
Cuts at: 0 370 876
Size: 370 506
BsiHKAI G_wGCw'C
Cuts at: 0 14 386 876
Size: 14 372 490
Fragments arranged by size:
490 372 14
Bsp1286I G_dGCh'C
Cuts at: 0 14 152 386 459 876
Size: 14 138 234 73 417
Fragments arranged by size:
417 234 138 73 14
BspEI T'CCGG_A
Cuts at: 0 629 876
Size: 629 247
BstYI r'GATC_y
Cuts at: 0 593 876
Size: 593 283
EarI CTCTTCn'nnn_
Cuts at: 0 217 386 876
Size: 217 169 490
Fragments arranged by size:
490 217 169
EcoICRI GAG'CTC
Cuts at: 0 12 384 876
Size: 12 372 492
Fragments arranged by size:
492 372 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 312 448 876
Size: 223 89 136 428
Fragments arranged by size:
428 223 136 89
EcoO109I rG'GnC_Cy
Cuts at: 0 231 455 456 876
Size: 231 224 1 420
Fragments arranged by size:
420 231 224 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 731 763 876
Size: 409 32 290 32 113
Fragments arranged by size:
409 290 113 32 32
HincII GTy'rAC
Cuts at: 0 758 876
Size: 758 118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 852 876
Size: 451 401 24
Fragments arranged by size:
451 401 24
MspA1I CmG'CkG
Cuts at: 0 287 876
Size: 287 589
NspI r_CATG'y
Cuts at: 0 714 876
Size: 714 162
PflMI CCAn_nnn'nTGG
Cuts at: 0 158 377 876
Size: 158 219 499
Fragments arranged by size:
499 219 158
PpuMI rG'GwC_Cy
Cuts at: 0 231 876
Size: 231 645
PspOMI G'GGCC_C
Cuts at: 0 455 876
Size: 455 421
PvuII CAG'CTG
Cuts at: 0 287 876
Size: 287 589
SacI G_AGCT'C
Cuts at: 0 14 386 876
Size: 14 372 490
Fragments arranged by size:
490 372 14
SfcI C'TryA_G
Cuts at: 0 597 876
Size: 597 279
SphI G_CATG'C
Cuts at: 0 714 876
Size: 714 162
StyI C'CwwG_G
Cuts at: 0 800 876
Size: 800 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 775 876
Size: 775 101
XmnI GAAnn'nnTTC
Cuts at: 0 564 876
Size: 564 312
Enzymes that do cut:
AlfI AlwNI ApaI BanII BbsI BbvCI BciVI BglII
Bme1580I BmrI BpmI Bpu10I BsaI BsaWI BseRI BseYI
BsiHKAI Bsp1286I BspEI BstYI EarI EcoICRI Eco57MI EcoO109I
Hin4I HincII MmeI MspA1I NspI PflMI PpuMI PspOMI
PvuII SacI SfcI SphI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BcgI BclI BfrBI BglI BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI