Restriction map for
pFN21AB7580
(Linear) (Six-base) MAPSORT of: KIBB7580.seq Check: 4496 from: 1 to: 852
KIBB7580.orf
With 182 enzymes: SgfI *
March 22, 2012 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 483 852
Size: 483 369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 505 852
Size: 505 347
ApaI G_GGCC'C
Cuts at: 0 352 852
Size: 352 500
ApoI r'AATT_y
Cuts at: 0 571 852
Size: 571 281
AvaI C'yCGr_G
Cuts at: 0 42 852
Size: 42 810
BamHI G'GATC_C
Cuts at: 0 186 852
Size: 186 666
BanI G'GyrC_C
Cuts at: 0 138 424 852
Size: 138 286 428
Fragments arranged by size:
428 286 138
BanII G_rGCy'C
Cuts at: 0 61 220 352 852
Size: 61 159 132 500
Fragments arranged by size:
500 159 132 61
BbeI G_GCGC'C
Cuts at: 0 428 852
Size: 428 424
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 852
Size: 248 604
BciVI GTATCCnnnnn_n'
Cuts at: 0 176 266 852
Size: 176 90 586
Fragments arranged by size:
586 176 90
BclI T'GATC_A
Cuts at: 0 543 692 852
Size: 543 149 160
Fragments arranged by size:
543 160 149
BfrBI ATG'CAT
Cuts at: 0 472 852
Size: 472 380
BglI GCCn_nnn'nGGC
Cuts at: 0 65 356 386 395 433 852
Size: 65 291 30 9 38 419
Fragments arranged by size:
419 291 65 38 30 9
Bme1580I G_kGCm'C
Cuts at: 0 49 283 352 852
Size: 49 234 69 500
Fragments arranged by size:
500 234 69 49
BmgBI CAC'GTC
Cuts at: 0 300 852
Size: 300 552
BmrI ACTGGGnnnn_n'
Cuts at: 0 705 852
Size: 705 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 852
Size: 267 585
BsaHI Gr'CG_yC
Cuts at: 0 425 852
Size: 425 427
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 719 736 749 766 852
Size: 719 17 13 17 86
Fragments arranged by size:
719 86 17 17 13
BseYI C'CCAG_C
Cuts at: 0 79 178 445 852
Size: 79 99 267 407
Fragments arranged by size:
407 267 99 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 852
Size: 511 341
BsiEI CG_ry'CG
Cuts at: 0 332 852
Size: 332 520
BsmBI CGTCTCn'nnnn_
Cuts at: 0 206 852
Size: 206 646
Bsp1286I G_dGCh'C
Cuts at: 0 49 61 220 283 352 852
Size: 49 12 159 63 69 500
Fragments arranged by size:
500 159 69 63 49 12
BspHI T'CATG_A
Cuts at: 0 53 852
Size: 53 799
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 825 852
Size: 483 342 27
Fragments arranged by size:
483 342 27
BsrDI GCAATG_nn'
Cuts at: 0 576 852
Size: 576 276
BsrFI r'CCGG_y
Cuts at: 0 141 288 852
Size: 141 147 564
Fragments arranged by size:
564 147 141
BstYI r'GATC_y
Cuts at: 0 186 852
Size: 186 666
EaeI y'GGCC_r
Cuts at: 0 196 852
Size: 196 656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 852
Size: 267 585
EcoO109I rG'GnC_Cy
Cuts at: 0 349 852
Size: 349 503
EcoRI G'AATT_C
Cuts at: 0 571 852
Size: 571 281
FspI TGC'GCA
Cuts at: 0 481 852
Size: 481 371
HaeII r_GCGC'y
Cuts at: 0 428 852
Size: 428 424
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 240 272 289 321 736 768 852
Size: 240 32 17 32 415 32 84
Fragments arranged by size:
415 240 84 32 32 32 17
HincII GTy'rAC
Cuts at: 0 624 852
Size: 624 228
HpaI GTT'AAC
Cuts at: 0 624 852
Size: 624 228
KasI G'GCGC_C
Cuts at: 0 424 852
Size: 424 428
MslI CAynn'nnrTG
Cuts at: 0 52 852
Size: 52 800
MspA1I CmG'CkG
Cuts at: 0 79 852
Size: 79 773
NarI GG'CG_CC
Cuts at: 0 425 852
Size: 425 427
NdeI CA'TA_TG
Cuts at: 0 173 852
Size: 173 679
NsiI A_TGCA'T
Cuts at: 0 474 852
Size: 474 378
NspI r_CATG'y
Cuts at: 0 39 852
Size: 39 813
PasI CC'CwG_GG
Cuts at: 0 61 763 852
Size: 61 702 89
Fragments arranged by size:
702 89 61
PspOMI G'GGCC_C
Cuts at: 0 348 852
Size: 348 504
PvuII CAG'CTG
Cuts at: 0 79 852
Size: 79 773
SfcI C'TryA_G
Cuts at: 0 225 336 852
Size: 225 111 516
Fragments arranged by size:
516 225 111
SfoI GGC'GCC
Cuts at: 0 426 852
Size: 426 426
SmaI CCC'GGG
Cuts at: 0 44 852
Size: 44 808
StyI C'CwwG_G
Cuts at: 0 431 677 852
Size: 431 246 175
Fragments arranged by size:
431 246 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 346 852
Size: 346 506
TatI w'GTAC_w
Cuts at: 0 271 852
Size: 271 581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 719 751 852
Size: 719 32 101
Fragments arranged by size:
719 101 32
XmaI C'CCGG_G
Cuts at: 0 42 852
Size: 42 810
Enzymes that do cut:
AarI AlfI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI Bme1580I BmgBI
BmrI BpmI BsaHI BsaXI BseYI BsgI BsiEI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BstYI EaeI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HpaI KasI
MslI MspA1I NarI NdeI NsiI NspI PasI PspOMI
PvuII SfcI SfoI SmaI StyI TaqII TatI TstI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI
BsiHKAI BsiWI BsmI BspEI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRV FalI FseI FspAI HindIII
KpnI MfeI MluI MmeI MscI NaeI NcoI NgoMIV
NheI NotI NruI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmnI ZraI