Restriction map for
pFN21AB7578
(Linear) (Six-base) MAPSORT of: KIBB7578.seq Check: 6250 from: 1 to: 852
KIBB7578.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 852
Size: 809 43
AflIII A'CryG_T
Cuts at: 0 710 852
Size: 710 142
AlwNI CAG_nnn'CTG
Cuts at: 0 72 304 507 792 852
Size: 72 232 203 285 60
Fragments arranged by size:
285 232 203 72 60
ApaI G_GGCC'C
Cuts at: 0 229 311 852
Size: 229 82 541
Fragments arranged by size:
541 229 82
AvaI C'yCGr_G
Cuts at: 0 150 171 312 852
Size: 150 21 141 540
Fragments arranged by size:
540 150 141 21
BanI G'GyrC_C
Cuts at: 0 291 325 638 671 852
Size: 291 34 313 33 181
Fragments arranged by size:
313 291 181 34 33
BanII G_rGCy'C
Cuts at: 0 229 311 839 852
Size: 229 82 528 13
Fragments arranged by size:
528 229 82 13
BbeI G_GCGC'C
Cuts at: 0 295 329 642 852
Size: 295 34 313 210
Fragments arranged by size:
313 295 210 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 151 852
Size: 151 701
BglI GCCn_nnn'nGGC
Cuts at: 0 233 434 729 852
Size: 233 201 295 123
Fragments arranged by size:
295 233 201 123
Bme1580I G_kGCm'C
Cuts at: 0 229 311 852
Size: 229 82 541
Fragments arranged by size:
541 229 82
BmgBI CAC'GTC
Cuts at: 0 485 852
Size: 485 367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 852
Size: 529 323
Bpu10I CC'TnA_GC
Cuts at: 0 73 852
Size: 73 779
BsaHI Gr'CG_yC
Cuts at: 0 292 326 639 852
Size: 292 34 313 213
Fragments arranged by size:
313 292 213 34
BsaWI w'CCGG_w
Cuts at: 0 217 452 852
Size: 217 235 400
Fragments arranged by size:
400 235 217
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 14 44 852
Size: 14 30 808
Fragments arranged by size:
808 30 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 667 852
Size: 667 185
BseYI C'CCAG_C
Cuts at: 0 15 283 852
Size: 15 268 569
Fragments arranged by size:
569 268 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 852
Size: 745 107
BsiEI CG_ry'CG
Cuts at: 0 372 724 733 852
Size: 372 352 9 119
Fragments arranged by size:
372 352 119 9
Bsp1286I G_dGCh'C
Cuts at: 0 229 311 839 852
Size: 229 82 528 13
Fragments arranged by size:
528 229 82 13
BspEI T'CCGG_A
Cuts at: 0 217 852
Size: 217 635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 800 852
Size: 800 52
BsrBI CCG'CTC
Cuts at: 0 77 497 519 669 852
Size: 77 420 22 150 183
Fragments arranged by size:
420 183 150 77 22
BsrFI r'CCGG_y
Cuts at: 0 187 389 683 852
Size: 187 202 294 169
Fragments arranged by size:
294 202 187 169
BssSI C'ACGA_G
Cuts at: 0 40 852
Size: 40 812
BstAPI GCAn_nnn'nTGC
Cuts at: 0 792 852
Size: 792 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 130 852
Size: 130 722
BstYI r'GATC_y
Cuts at: 0 68 852
Size: 68 784
BtgI C'CryG_G
Cuts at: 0 162 718 852
Size: 162 556 134
Fragments arranged by size:
556 162 134
BtsI GCAGTG_nn'
Cuts at: 0 305 852
Size: 305 547
ClaI AT'CG_AT
Cuts at: 0 62 852
Size: 62 790
DraIII CAC_nnn'GTG
Cuts at: 0 632 852
Size: 632 220
DrdI GACnn_nn'nnGTC
Cuts at: 0 598 852
Size: 598 254
EaeI y'GGCC_r
Cuts at: 0 369 569 721 730 852
Size: 369 200 152 9 122
Fragments arranged by size:
369 200 152 122 9
EagI C'GGCC_G
Cuts at: 0 369 721 730 852
Size: 369 352 9 122
Fragments arranged by size:
369 352 122 9
EarI CTCTTCn'nnn_
Cuts at: 0 781 852
Size: 781 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 664 852
Size: 664 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 809 852
Size: 529 280 43
Fragments arranged by size:
529 280 43
EcoO109I rG'GnC_Cy
Cuts at: 0 308 852
Size: 308 544
FspI TGC'GCA
Cuts at: 0 254 583 852
Size: 254 329 269
Fragments arranged by size:
329 269 254
HaeII r_GCGC'y
Cuts at: 0 295 329 525 568 642 852
Size: 295 34 196 43 74 210
Fragments arranged by size:
295 210 196 74 43 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 852
Size: 3 32 817
Fragments arranged by size:
817 32 3
KasI G'GCGC_C
Cuts at: 0 291 325 638 852
Size: 291 34 313 214
Fragments arranged by size:
313 291 214 34
MluI A'CGCG_T
Cuts at: 0 710 852
Size: 710 142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 852
Size: 494 358
MscI TGG'CCA
Cuts at: 0 571 852
Size: 571 281
MspA1I CmG'CkG
Cuts at: 0 204 264 283 368 726 738 852
Size: 204 60 19 85 358 12 114
Fragments arranged by size:
358 204 114 85 60 19 12
NaeI GCC'GGC
Cuts at: 0 189 685 852
Size: 189 496 167
Fragments arranged by size:
496 189 167
NarI GG'CG_CC
Cuts at: 0 292 326 639 852
Size: 292 34 313 213
Fragments arranged by size:
313 292 213 34
NgoMIV G'CCGG_C
Cuts at: 0 187 683 852
Size: 187 496 169
Fragments arranged by size:
496 187 169
NotI GC'GGCC_GC
Cuts at: 0 369 730 852
Size: 369 361 122
Fragments arranged by size:
369 361 122
PasI CC'CwG_GG
Cuts at: 0 91 800 852
Size: 91 709 52
Fragments arranged by size:
709 91 52
PspOMI G'GGCC_C
Cuts at: 0 225 307 852
Size: 225 82 545
Fragments arranged by size:
545 225 82
PstI C_TGCA'G
Cuts at: 0 300 704 788 797 852
Size: 300 404 84 9 55
Fragments arranged by size:
404 300 84 55 9
SapI GCTCTTCn'nnn_
Cuts at: 0 781 852
Size: 781 71
SfcI C'TryA_G
Cuts at: 0 296 700 784 793 852
Size: 296 404 84 9 59
Fragments arranged by size:
404 296 84 59 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 729 852
Size: 729 123
SfoI GGC'GCC
Cuts at: 0 293 327 640 852
Size: 293 34 313 212
Fragments arranged by size:
313 293 212 34
StuI AGG'CCT
Cuts at: 0 107 335 577 852
Size: 107 228 242 275
Fragments arranged by size:
275 242 228 107
StyI C'CwwG_G
Cuts at: 0 383 572 852
Size: 383 189 280
Fragments arranged by size:
383 280 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 604 852
Size: 604 248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 852
Size: 14 32 806
Fragments arranged by size:
806 32 14
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvaI BanI BanII BbeI
BciVI BglI Bme1580I BmgBI BpmI Bpu10I BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspEI BspMI
BsrBI BsrFI BssSI BstAPI BstXI BstYI BtgI BtsI
ClaI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I FspI HaeII Hin4I KasI MluI MmeI MscI
MspA1I NaeI NarI NgoMIV NotI PasI PspOMI PstI
SapI SfcI SfiI SfoI StuI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BclI
BfrBI BglII BlpI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsiHKAI BsiWI BsmI BsmBI BspHI BsrDI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI CspCI
DraI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MslI NcoI NdeI
NheI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI