Restriction map for
pFN21AB7560
(Linear) (Six-base) MAPSORT of: KIBB7560.seq Check: 3916 from: 1 to: 816
KIBB7560.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
Acc65I G'GTAC_C
Cuts at: 0 215 816
Size: 215 601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 170 816
Size: 170 646
AlwNI CAG_nnn'CTG
Cuts at: 0 546 816
Size: 546 270
AvaI C'yCGr_G
Cuts at: 0 181 351 595 816
Size: 181 170 244 221
Fragments arranged by size:
244 221 181 170
BanI G'GyrC_C
Cuts at: 0 37 96 215 376 816
Size: 37 59 119 161 440
Fragments arranged by size:
440 161 119 59 37
BbeI G_GCGC'C
Cuts at: 0 41 100 380 816
Size: 41 59 280 436
Fragments arranged by size:
436 280 59 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 620 816
Size: 586 34 196
Fragments arranged by size:
586 196 34
BglI GCCn_nnn'nGGC
Cuts at: 0 109 816
Size: 109 707
Bme1580I G_kGCm'C
Cuts at: 0 56 816
Size: 56 760
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 366 816
Size: 366 450
BsaI GGTCTCn'nnnn_
Cuts at: 0 436 816
Size: 436 380
BsaHI Gr'CG_yC
Cuts at: 0 38 97 377 816
Size: 38 59 280 439
Fragments arranged by size:
439 280 59 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 604 634 816
Size: 604 30 182
Fragments arranged by size:
604 182 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 355 816
Size: 262 93 461
Fragments arranged by size:
461 262 93
BseYI C'CCAG_C
Cuts at: 0 157 253 816
Size: 157 96 563
Fragments arranged by size:
563 157 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 626 816
Size: 405 221 190
Fragments arranged by size:
405 221 190
BsiEI CG_ry'CG
Cuts at: 0 116 816
Size: 116 700
Bsp1286I G_dGCh'C
Cuts at: 0 56 816
Size: 56 760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 816
Size: 427 389
BsrGI T'GTAC_A
Cuts at: 0 506 816
Size: 506 310
BssSI C'ACGA_G
Cuts at: 0 447 816
Size: 447 369
BstAPI GCAn_nnn'nTGC
Cuts at: 0 419 816
Size: 419 397
BstYI r'GATC_y
Cuts at: 0 327 643 816
Size: 327 316 173
Fragments arranged by size:
327 316 173
BtgI C'CryG_G
Cuts at: 0 62 116 475 816
Size: 62 54 359 341
Fragments arranged by size:
359 341 62 54
EaeI y'GGCC_r
Cuts at: 0 113 458 816
Size: 113 345 358
Fragments arranged by size:
358 345 113
EagI C'GGCC_G
Cuts at: 0 113 816
Size: 113 703
EarI CTCTTCn'nnn_
Cuts at: 0 239 526 816
Size: 239 287 290
Fragments arranged by size:
290 287 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 283 816
Size: 283 533
EcoO109I rG'GnC_Cy
Cuts at: 0 543 816
Size: 543 273
HaeII r_GCGC'y
Cuts at: 0 41 100 380 816
Size: 41 59 280 436
Fragments arranged by size:
436 280 59 41
KasI G'GCGC_C
Cuts at: 0 37 96 376 816
Size: 37 59 280 440
Fragments arranged by size:
440 280 59 37
KpnI G_GTAC'C
Cuts at: 0 219 816
Size: 219 597
MscI TGG'CCA
Cuts at: 0 460 816
Size: 460 356
MspA1I CmG'CkG
Cuts at: 0 47 64 118 371 395 816
Size: 47 17 54 253 24 421
Fragments arranged by size:
421 253 54 47 24 17
NarI GG'CG_CC
Cuts at: 0 38 97 377 816
Size: 38 59 280 439
Fragments arranged by size:
439 280 59 38
NotI GC'GGCC_GC
Cuts at: 0 113 816
Size: 113 703
PfoI T'CCnGG_A
Cuts at: 0 801 816
Size: 801 15
PspXI vC'TCGA_Gb
Cuts at: 0 595 816
Size: 595 221
PstI C_TGCA'G
Cuts at: 0 439 816
Size: 439 377
SacII CC_GC'GG
Cuts at: 0 65 119 816
Size: 65 54 697
Fragments arranged by size:
697 65 54
SapI GCTCTTCn'nnn_
Cuts at: 0 526 816
Size: 526 290
SfcI C'TryA_G
Cuts at: 0 435 816
Size: 435 381
SfoI GGC'GCC
Cuts at: 0 39 98 378 816
Size: 39 59 280 438
Fragments arranged by size:
438 280 59 39
SmaI CCC'GGG
Cuts at: 0 183 816
Size: 183 633
SmlI C'TyrA_G
Cuts at: 0 595 816
Size: 595 221
StyI C'CwwG_G
Cuts at: 0 92 816
Size: 92 724
TatI w'GTAC_w
Cuts at: 0 506 816
Size: 506 310
XbaI T'CTAG_A
Cuts at: 0 330 816
Size: 330 486
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 816
Size: 453 363
XhoI C'TCGA_G
Cuts at: 0 595 816
Size: 595 221
XmaI C'CCGG_G
Cuts at: 0 181 816
Size: 181 635
Enzymes that do cut:
Acc65I AlfI AlwNI AvaI BanI BbeI BcgI BglI
Bme1580I BplI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI Bsp1286I BspMI BsrGI BssSI BstAPI BstYI BtgI
EaeI EagI EarI EciI EcoO109I HaeII KasI KpnI
MscI MspA1I NarI NotI PfoI PspXI PstI SacII
SapI SfcI SfoI SmaI SmlI StyI TatI XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI MfeI MluI MmeI MslI NaeI NcoI NdeI
NgoMIV NheI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XmnI
ZraI