(Linear) (Six-base) MAPSORT of: KIBB7520.seq  Check: 6777  from: 1  to: 744



KIBB7520.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:29  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     744 
   Size:         67     677
 
AflIII A'CryG_T

Cuts at:      0       4     744 
   Size:          4     740
 
ApoI r'AATT_y

Cuts at:      0     147     744 
   Size:        147     597
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     490     744 
   Size:        490     254
 
BclI T'GATC_A

Cuts at:      0     657     744 
   Size:        657      87
 
BglII A'GATC_T

Cuts at:      0      78     629     744 
   Size:         78     551     115

  Fragments arranged by size: 

                 551     115      78
 
Bme1580I G_kGCm'C

Cuts at:      0     516     744 
   Size:        516     228
 
BmgBI CAC'GTC

Cuts at:      0       5     744 
   Size:          5     739
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     283     744 
   Size:         95     188     461

  Fragments arranged by size: 

                 461     188      95
 
BsaWI w'CCGG_w

Cuts at:      0     369     554     744 
   Size:        369     185     190

  Fragments arranged by size: 

                 369     190     185
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     193     744 
   Size:        193     551
 
BseYI C'CCAG_C

Cuts at:      0      24     744 
   Size:         24     720
 
BsmI GAATG_Cn'

Cuts at:      0     693     744 
   Size:        693      51
 
Bsp1286I G_dGCh'C

Cuts at:      0     516     744 
   Size:        516     228
 
BspEI T'CCGG_A

Cuts at:      0     369     554     744 
   Size:        369     185     190

  Fragments arranged by size: 

                 369     190     185
 
BstYI r'GATC_y

Cuts at:      0      78     629     744 
   Size:         78     551     115

  Fragments arranged by size: 

                 551     115      78
 
DraI TTT'AAA

Cuts at:      0     677     744 
   Size:        677      67
 
DraIII CAC_nnn'GTG

Cuts at:      0     646     744 
   Size:        646      98
 
EaeI y'GGCC_r

Cuts at:      0     506     744 
   Size:        506     238
 
EarI CTCTTCn'nnn_

Cuts at:      0     299     744 
   Size:        299     445
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67      95     283     744 
   Size:         67      28     188     461

  Fragments arranged by size: 

                 461     188      67      28
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     463     744 
   Size:        463     281
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     364     396     744 
   Size:        364      32     348

  Fragments arranged by size: 

                 364     348      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     744 
   Size:        357     387
 
MscI TGG'CCA

Cuts at:      0     508     744 
   Size:        508     236
 
MspA1I CmG'CkG

Cuts at:      0     155     279     744 
   Size:        155     124     465

  Fragments arranged by size: 

                 465     155     124
 
NdeI CA'TA_TG

Cuts at:      0     708     744 
   Size:        708      36
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     504     744 
   Size:        504     240
 
PsiI TTA'TAA

Cuts at:      0     698     744 
   Size:        698      46
 
PstI C_TGCA'G

Cuts at:      0     260     744 
   Size:        260     484
 
PvuII CAG'CTG

Cuts at:      0     155     279     744 
   Size:        155     124     465

  Fragments arranged by size: 

                 465     155     124
 
ScaI AGT'ACT

Cuts at:      0      88     744 
   Size:         88     656
 
SfcI C'TryA_G

Cuts at:      0     173     256     744 
   Size:        173      83     488

  Fragments arranged by size: 

                 488     173      83
 
SspI AAT'ATT

Cuts at:      0     243     744 
   Size:        243     501
 
TatI w'GTAC_w

Cuts at:      0      86     744 
   Size:         86     658
 
XmnI GAAnn'nnTTC

Cuts at:      0     347     744 
   Size:        347     397
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     BbsI     BclI    BglII Bme1580I    BmgBI
     BpmI    BsaWI    BseRI    BseYI     BsmI Bsp1286I    BspEI    BstYI
     DraI   DraIII     EaeI     EarI  Eco57MI     FalI    Hin4I     MmeI
     MscI   MspA1I     NdeI    PflMI     PsiI     PstI    PvuII     ScaI
     SfcI     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI