(Linear) (Six-base) MAPSORT of: KIBB7517.seq  Check: 7335  from: 1  to: 330



KIBB7517.orf

 With 182 enzymes: SgfI * 

                        September 21, 2017 11:26  ..

AccI GT'mk_AC

Cuts at:      0      17     155     330 
   Size:         17     138     175

  Fragments arranged by size: 

                 175     138      17
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     330 
   Size:        293      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     319     330 
   Size:        319      11
 
BsaHI Gr'CG_yC

Cuts at:      0      95     330 
   Size:         95     235
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      10      40     330 
   Size:         10      30     290

  Fragments arranged by size: 

                 290      30      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     205     330 
   Size:        202       3     125

  Fragments arranged by size: 

                 202     125       3
 
BsmI GAATG_Cn'

Cuts at:      0     117     330 
   Size:        117     213
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      86     330 
   Size:         86     244
 
EarI CTCTTCn'nnn_

Cuts at:      0      68      84     158     179     212     330 
   Size:         68      16      74      21      33     118

  Fragments arranged by size: 

                 118      74      68      33      21      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     330 
   Size:        293      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     330 
   Size:         10      32     288

  Fragments arranged by size: 

                 288      32      10
 
MspA1I CmG'CkG

Cuts at:      0     271     330 
   Size:        271      59
 
PvuII CAG'CTG

Cuts at:      0     271     330 
   Size:        271      59
 
StyI C'CwwG_G

Cuts at:      0      41     330 
   Size:         41     289
 
Tth111I GACn'n_nGTC

Cuts at:      0     292     330 
   Size:        292      38
 

 Enzymes that do cut:

     AccI     AcuI     BbsI    BsaHI    BsaXI    BseRI     BsmI    BsmBI
     EarI  Eco57MI    Hin4I   MspA1I    PvuII     StyI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI