Restriction map for
pFN21AB7309
(Linear) (Six-base) MAPSORT of: KIBB7309.seq Check: 3128 from: 1 to: 2733
KIBB7309.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2466 2733
Size: 2466 267
AccI GT'mk_AC
Cuts at: 0 46 2733
Size: 46 2687
AflIII A'CryG_T
Cuts at: 0 702 1577 2716 2733
Size: 702 875 1139 17
Fragments arranged by size:
1139 875 702 17
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1622 2733
Size: 1622 1111
AlwNI CAG_nnn'CTG
Cuts at: 0 70 571 969 1602 1617 2376 2733
Size: 70 501 398 633 15 759 357
Fragments arranged by size:
759 633 501 398 357 70 15
ApaI G_GGCC'C
Cuts at: 0 1945 2733
Size: 1945 788
ApaLI G'TGCA_C
Cuts at: 0 344 2174 2733
Size: 344 1830 559
Fragments arranged by size:
1830 559 344
ApoI r'AATT_y
Cuts at: 0 300 667 2678 2733
Size: 300 367 2011 55
Fragments arranged by size:
2011 367 300 55
AvaI C'yCGr_G
Cuts at: 0 18 2733
Size: 18 2715
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2241 2274 2733
Size: 2241 33 459
Fragments arranged by size:
2241 459 33
BamHI G'GATC_C
Cuts at: 0 929 2733
Size: 929 1804
BanII G_rGCy'C
Cuts at: 0 1945 2107 2453 2557 2733
Size: 1945 162 346 104 176
Fragments arranged by size:
1945 346 176 162 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 40 1574 2444 2733
Size: 40 1534 870 289
Fragments arranged by size:
1534 870 289 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 942 976 2733
Size: 942 34 1757
Fragments arranged by size:
1757 942 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1111 2733
Size: 1111 1622
BglI GCCn_nnn'nGGC
Cuts at: 0 1489 1695 2733
Size: 1489 206 1038
Fragments arranged by size:
1489 1038 206
BglII A'GATC_T
Cuts at: 0 389 902 2733
Size: 389 513 1831
Fragments arranged by size:
1831 513 389
Bme1580I G_kGCm'C
Cuts at: 0 348 1611 1945 2178 2311 2733
Size: 348 1263 334 233 133 422
Fragments arranged by size:
1263 422 348 334 233 133
BmgBI CAC'GTC
Cuts at: 0 1580 2733
Size: 1580 1153
BmrI ACTGGGnnnn_n'
Cuts at: 0 384 2005 2043 2733
Size: 384 1621 38 690
Fragments arranged by size:
1621 690 384 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1331 2733
Size: 1331 1402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 84 568 1572 1639 1785 2020 2469
Size: 47 37 484 1004 67 146 235 449
Cuts at: 2469 2733
Size: 264
Fragments arranged by size:
1004 484 449 264 235 146 67 47
37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 2733
Size: 1339 1394
BsaI GGTCTCn'nnnn_
Cuts at: 0 2519 2733
Size: 2519 214
BsaAI yAC'GTr
Cuts at: 0 705 2719 2733
Size: 705 2014 14
Fragments arranged by size:
2014 705 14
BsaBI GATnn'nnATC
Cuts at: 0 272 756 2733
Size: 272 484 1977
Fragments arranged by size:
1977 484 272
BsaWI w'CCGG_w
Cuts at: 0 83 308 2733
Size: 83 225 2425
Fragments arranged by size:
2425 225 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 700 730 2733
Size: 700 30 2003
Fragments arranged by size:
2003 700 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2415 2418 2482 2733
Size: 2415 3 64 251
Fragments arranged by size:
2415 251 64 3
BseYI C'CCAG_C
Cuts at: 0 1210 2191 2304 2733
Size: 1210 981 113 429
Fragments arranged by size:
1210 981 429 113
BsiHKAI G_wGCw'C
Cuts at: 0 348 1798 1928 2107 2178 2453 2733
Size: 348 1450 130 179 71 275 280
Fragments arranged by size:
1450 348 280 275 179 130 71
BsmI GAATG_Cn'
Cuts at: 0 948 2733
Size: 948 1785
Bsp1286I G_dGCh'C
Cuts at: 0 348 1611 1798 1928 1945 2107 2178 2311
Size: 348 1263 187 130 17 162 71 133
Cuts at: 2311 2453 2557 2733
Size: 142 104 176
Fragments arranged by size:
1263 348 187 176 162 142 133 130
104 71 17
BspEI T'CCGG_A
Cuts at: 0 83 2733
Size: 83 2650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 517 1986 2466 2733
Size: 517 1469 480 267
Fragments arranged by size:
1469 517 480 267
BsrBI CCG'CTC
Cuts at: 0 1323 2733
Size: 1323 1410
BsrDI GCAATG_nn'
Cuts at: 0 620 2733
Size: 620 2113
BsrFI r'CCGG_y
Cuts at: 0 1469 2733
Size: 1469 1264
BssSI C'ACGA_G
Cuts at: 0 208 1561 2733
Size: 208 1353 1172
Fragments arranged by size:
1353 1172 208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 95 2733
Size: 95 2638
BstXI CCAn_nnnn'nTGG
Cuts at: 0 773 1472 2733
Size: 773 699 1261
Fragments arranged by size:
1261 773 699
BstYI r'GATC_y
Cuts at: 0 389 578 902 929 2137 2733
Size: 389 189 324 27 1208 596
Fragments arranged by size:
1208 596 389 324 189 27
Bsu36I CC'TnA_GG
Cuts at: 0 925 982 2733
Size: 925 57 1751
Fragments arranged by size:
1751 925 57
BtgI C'CryG_G
Cuts at: 0 734 1028 1760 2733
Size: 734 294 732 973
Fragments arranged by size:
973 734 732 294
BtsI GCAGTG_nn'
Cuts at: 0 2178 2206 2553 2733
Size: 2178 28 347 180
Fragments arranged by size:
2178 347 180 28
DraI TTT'AAA
Cuts at: 0 299 2733
Size: 299 2434
DrdI GACnn_nn'nnGTC
Cuts at: 0 42 2733
Size: 42 2691
EaeI y'GGCC_r
Cuts at: 0 357 584 815 1025 1757 2733
Size: 357 227 231 210 732 976
Fragments arranged by size:
976 732 357 231 227 210
EarI CTCTTCn'nnn_
Cuts at: 0 488 1709 1786 1916 2394 2534 2562 2733
Size: 488 1221 77 130 478 140 28 171
Fragments arranged by size:
1221 488 478 171 140 130 77 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1002 1413 1431 1863 2733
Size: 1002 411 18 432 870
Fragments arranged by size:
1002 870 432 411 18
EcoICRI GAG'CTC
Cuts at: 0 2105 2451 2733
Size: 2105 346 282
Fragments arranged by size:
2105 346 282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 84 568 1572 1639 1785 2020 2469
Size: 47 37 484 1004 67 146 235 449
Cuts at: 2469 2733
Size: 264
Fragments arranged by size:
1004 484 449 264 235 146 67 47
37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2342 2733
Size: 2342 391
EcoO109I rG'GnC_Cy
Cuts at: 0 126 1941 1942 1975 2335 2733
Size: 126 1815 1 33 360 398
Fragments arranged by size:
1815 398 360 126 33 1
EcoRV GAT'ATC
Cuts at: 0 421 2733
Size: 421 2312
FspI TGC'GCA
Cuts at: 0 919 2733
Size: 919 1814
FspAI rTGC'GCAy
Cuts at: 0 919 2733
Size: 919 1814
HaeII r_GCGC'y
Cuts at: 0 2326 2733
Size: 2326 407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 1638 1670 1684 1716 2643 2675
Size: 98 32 1508 32 14 32 927 32
Cuts at: 2675 2733
Size: 58
Fragments arranged by size:
1508 927 98 58 32 32 32 32
14
HincII GTy'rAC
Cuts at: 0 47 2016 2733
Size: 47 1969 717
Fragments arranged by size:
1969 717 47
HindIII A'AGCT_T
Cuts at: 0 187 2733
Size: 187 2546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1478 1535 2711 2733
Size: 881 597 57 1176 22
Fragments arranged by size:
1176 881 597 57 22
MscI TGG'CCA
Cuts at: 0 359 586 817 1027 2733
Size: 359 227 231 210 1706
Fragments arranged by size:
1706 359 231 227 210
MslI CAynn'nnrTG
Cuts at: 0 1116 1132 1604 2023 2733
Size: 1116 16 472 419 710
Fragments arranged by size:
1116 710 472 419 16
MspA1I CmG'CkG
Cuts at: 0 1569 1755 1823 1860 2224 2733
Size: 1569 186 68 37 364 509
Fragments arranged by size:
1569 509 364 186 68 37
NcoI C'CATG_G
Cuts at: 0 734 1028 2733
Size: 734 294 1705
Fragments arranged by size:
1705 734 294
NdeI CA'TA_TG
Cuts at: 0 326 1707 2733
Size: 326 1381 1026
Fragments arranged by size:
1381 1026 326
NspI r_CATG'y
Cuts at: 0 98 2733
Size: 98 2635
PasI CC'CwG_GG
Cuts at: 0 1912 2286 2455 2733
Size: 1912 374 169 278
Fragments arranged by size:
1912 374 278 169
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 1617 2733
Size: 330 1287 1116
Fragments arranged by size:
1287 1116 330
PfoI T'CCnGG_A
Cuts at: 0 100 218 276 2733
Size: 100 118 58 2457
Fragments arranged by size:
2457 118 100 58
PmlI CAC'GTG
Cuts at: 0 705 2719 2733
Size: 705 2014 14
Fragments arranged by size:
2014 705 14
PpuMI rG'GwC_Cy
Cuts at: 0 1975 2335 2733
Size: 1975 360 398
Fragments arranged by size:
1975 398 360
PshAI GACnn'nnGTC
Cuts at: 0 1774 2576 2633 2733
Size: 1774 802 57 100
Fragments arranged by size:
1774 802 100 57
PspOMI G'GGCC_C
Cuts at: 0 1941 2733
Size: 1941 792
PstI C_TGCA'G
Cuts at: 0 656 875 965 2733
Size: 656 219 90 1768
Fragments arranged by size:
1768 656 219 90
PvuII CAG'CTG
Cuts at: 0 1569 1755 1823 2224 2733
Size: 1569 186 68 401 509
Fragments arranged by size:
1569 509 401 186 68
RsrII CG'GwC_CG
Cuts at: 0 311 2733
Size: 311 2422
SacI G_AGCT'C
Cuts at: 0 2107 2453 2733
Size: 2107 346 280
Fragments arranged by size:
2107 346 280
SalI G'TCGA_C
Cuts at: 0 45 2733
Size: 45 2688
SapI GCTCTTCn'nnn_
Cuts at: 0 1786 1916 2562 2733
Size: 1786 130 646 171
Fragments arranged by size:
1786 646 171 130
SfcI C'TryA_G
Cuts at: 0 652 871 961 2586 2733
Size: 652 219 90 1625 147
Fragments arranged by size:
1625 652 219 147 90
SmaI CCC'GGG
Cuts at: 0 20 2733
Size: 20 2713
SmlI C'TyrA_G
Cuts at: 0 1318 2733
Size: 1318 1415
StuI AGG'CCT
Cuts at: 0 959 2733
Size: 959 1774
StyI C'CwwG_G
Cuts at: 0 734 1028 2733
Size: 734 294 1705
Fragments arranged by size:
1705 734 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1679 1786 2733
Size: 1679 107 947
Fragments arranged by size:
1679 947 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 325 357 700 732 2079 2111 2733
Size: 325 32 343 32 1347 32 622
Fragments arranged by size:
1347 622 343 325 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 852 2733
Size: 852 1881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 177 2733
Size: 177 2556
XmaI C'CCGG_G
Cuts at: 0 18 2733
Size: 18 2715
XmnI GAAnn'nnTTC
Cuts at: 0 781 2377 2733
Size: 781 1596 356
Fragments arranged by size:
1596 781 356
Enzymes that do cut:
AarI AccI AflIII AlfI AlwNI ApaI ApaLI ApoI
AvaI BaeI BamHI BanII BbsI BcgI BciVI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI
FspAI HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NcoI NdeI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII RsrII SacI SalI
SapI SfcI SmaI SmlI StuI StyI TaqII TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI AscI AseI AsiSI AvrII BanI BbeI
BbvCI BclI BfrBI BlpI BmtI Bpu10I BsaHI BsgI
BsiEI BsiWI BsmBI BspHI BsrGI BssHII BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraIII EagI EcoRI FalI
FseI HpaI KasI KpnI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PsiI PspXI PsrI PvuI SacII SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XhoI ZraI