Restriction map for
pFN21AB7308
(Linear) (Six-base) MAPSORT of: KIBB7308.seq Check: 489 from: 1 to: 2466
KIBB7308.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 315 2466
Size: 315 2151
AccI GT'mk_AC
Cuts at: 0 1898 2040 2466
Size: 1898 142 426
Fragments arranged by size:
1898 426 142
Acc65I G'GTAC_C
Cuts at: 0 1379 2466
Size: 1379 1087
AclI AA'CG_TT
Cuts at: 0 931 2466
Size: 931 1535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 853 1139 1307 2244 2413 2466
Size: 40 813 286 168 937 169 53
Fragments arranged by size:
937 813 286 169 168 53 40
AflIII A'CryG_T
Cuts at: 0 1535 1774 2225 2466
Size: 1535 239 451 241
Fragments arranged by size:
1535 451 241 239
AhdI GACnn_n'nnGTC
Cuts at: 0 1894 2466
Size: 1894 572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2082 2466
Size: 2082 384
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2196 2228 2466
Size: 2196 32 238
Fragments arranged by size:
2196 238 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1107 2466
Size: 1107 1359
ApoI r'AATT_y
Cuts at: 0 24 88 441 1258 1716 1810 1829 1987
Size: 24 64 353 817 458 94 19 158
Cuts at: 1987 2466
Size: 479
Fragments arranged by size:
817 479 458 353 158 94 64 24
19
AseI AT'TA_AT
Cuts at: 0 427 2466
Size: 427 2039
AvaI C'yCGr_G
Cuts at: 0 2023 2466
Size: 2023 443
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1371 1404 2466
Size: 1371 33 1062
Fragments arranged by size:
1371 1062 33
BanI G'GyrC_C
Cuts at: 0 1379 2466
Size: 1379 1087
BanII G_rGCy'C
Cuts at: 0 1321 2441 2466
Size: 1321 1120 25
Fragments arranged by size:
1321 1120 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 262 2466
Size: 262 2204
BciVI GTATCCnnnnn_n'
Cuts at: 0 210 2320 2466
Size: 210 2110 146
Fragments arranged by size:
2110 210 146
BclI T'GATC_A
Cuts at: 0 374 1845 2466
Size: 374 1471 621
Fragments arranged by size:
1471 621 374
BfrBI ATG'CAT
Cuts at: 0 127 2466
Size: 127 2339
BglII A'GATC_T
Cuts at: 0 1785 2466
Size: 1785 681
BlpI GC'TnA_GC
Cuts at: 0 1060 2466
Size: 1060 1406
Bme1580I G_kGCm'C
Cuts at: 0 576 1555 1741 2466
Size: 576 979 186 725
Fragments arranged by size:
979 725 576 186
BmgBI CAC'GTC
Cuts at: 0 2226 2466
Size: 2226 240
BmrI ACTGGGnnnn_n'
Cuts at: 0 66 1324 1565 1714 2466
Size: 66 1258 241 149 752
Fragments arranged by size:
1258 752 241 149 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 2466
Size: 1175 1291
Bpu10I CC'TnA_GC
Cuts at: 0 2265 2466
Size: 2265 201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1394 1819 2466
Size: 1394 425 647
Fragments arranged by size:
1394 647 425
BsaI GGTCTCn'nnnn_
Cuts at: 0 1304 2248 2466
Size: 1304 944 218
Fragments arranged by size:
1304 944 218
BsaBI GATnn'nnATC
Cuts at: 0 1345 2466
Size: 1345 1121
BsaWI w'CCGG_w
Cuts at: 0 2184 2330 2466
Size: 2184 146 136
Fragments arranged by size:
2184 146 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2196 2226 2466
Size: 2196 30 240
Fragments arranged by size:
2196 240 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 994 1518 2466
Size: 994 524 948
Fragments arranged by size:
994 948 524
BseYI C'CCAG_C
Cuts at: 0 2345 2466
Size: 2345 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1295 2466
Size: 1295 1171
BsiHKAI G_wGCw'C
Cuts at: 0 2441 2466
Size: 2441 25
Bsp1286I G_dGCh'C
Cuts at: 0 576 1321 1555 1741 2441 2466
Size: 576 745 234 186 700 25
Fragments arranged by size:
745 700 576 234 186 25
BspHI T'CATG_A
Cuts at: 0 1677 2375 2466
Size: 1677 698 91
Fragments arranged by size:
1677 698 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 315 2466
Size: 315 2151
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1161 2466
Size: 1161 1305
BstEII G'GTnAC_C
Cuts at: 0 381 2466
Size: 381 2085
BstYI r'GATC_y
Cuts at: 0 762 1785 2466
Size: 762 1023 681
Fragments arranged by size:
1023 762 681
BstZ17I GTA'TAC
Cuts at: 0 2041 2466
Size: 2041 425
Bsu36I CC'TnA_GG
Cuts at: 0 1652 1793 2466
Size: 1652 141 673
Fragments arranged by size:
1652 673 141
BtgI C'CryG_G
Cuts at: 0 1383 2466
Size: 1383 1083
BtsI GCAGTG_nn'
Cuts at: 0 44 2466
Size: 44 2422
ClaI AT'CG_AT
Cuts at: 0 1542 2466
Size: 1542 924
DraI TTT'AAA
Cuts at: 0 971 2466
Size: 971 1495
EarI CTCTTCn'nnn_
Cuts at: 0 997 1326 2466
Size: 997 329 1140
Fragments arranged by size:
1140 997 329
EcoICRI GAG'CTC
Cuts at: 0 2439 2466
Size: 2439 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 853 1139 1175 1307 2244 2413 2466
Size: 40 813 286 36 132 937 169 53
Fragments arranged by size:
937 813 286 169 132 53 40 36
EcoO109I rG'GnC_Cy
Cuts at: 0 2049 2466
Size: 2049 417
EcoRI G'AATT_C
Cuts at: 0 24 441 2466
Size: 24 417 2025
Fragments arranged by size:
2025 417 24
EcoRV GAT'ATC
Cuts at: 0 603 2466
Size: 603 1863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1804 2466
Size: 1804 662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 262 294 684 716 1024 1056 1204 1236
Size: 262 32 390 32 308 32 148 32
Cuts at: 1236 1237 1269 1327 1359 1499 1531 1659 1691
Size: 1 32 58 32 140 32 128 32
Cuts at: 1691 1880 1912 2325 2357 2466
Size: 189 32 413 32 109
Fragments arranged by size:
413 390 308 262 189 148 140 128
109 58 32 32 32 32 32 32
32 32 32 32 1
HindIII A'AGCT_T
Cuts at: 0 467 2466
Size: 467 1999
KpnI G_GTAC'C
Cuts at: 0 1383 2466
Size: 1383 1083
MfeI C'AATT_G
Cuts at: 0 1280 2466
Size: 1280 1186
MslI CAynn'nnrTG
Cuts at: 0 304 1682 1906 2466
Size: 304 1378 224 560
Fragments arranged by size:
1378 560 304 224
MspA1I CmG'CkG
Cuts at: 0 1107 2466
Size: 1107 1359
NcoI C'CATG_G
Cuts at: 0 1383 2466
Size: 1383 1083
NdeI CA'TA_TG
Cuts at: 0 536 2466
Size: 536 1930
NsiI A_TGCA'T
Cuts at: 0 129 2466
Size: 129 2337
NspI r_CATG'y
Cuts at: 0 265 1539 1778 2466
Size: 265 1274 239 688
Fragments arranged by size:
1274 688 265 239
PciI A'CATG_T
Cuts at: 0 1535 1774 2466
Size: 1535 239 692
Fragments arranged by size:
1535 692 239
PflMI CCAn_nnn'nTGG
Cuts at: 0 209 2174 2466
Size: 209 1965 292
Fragments arranged by size:
1965 292 209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1955 1987 2196 2228 2466
Size: 1955 32 209 32 238
Fragments arranged by size:
1955 238 209 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2049 2466
Size: 2049 417
PsiI TTA'TAA
Cuts at: 0 2396 2466
Size: 2396 70
PvuII CAG'CTG
Cuts at: 0 1107 2466
Size: 1107 1359
SacI G_AGCT'C
Cuts at: 0 2441 2466
Size: 2441 25
SapI GCTCTTCn'nnn_
Cuts at: 0 1326 2466
Size: 1326 1140
ScaI AGT'ACT
Cuts at: 0 171 2466
Size: 171 2295
SexAI A'CCwGG_T
Cuts at: 0 1464 2466
Size: 1464 1002
SfcI C'TryA_G
Cuts at: 0 417 2466
Size: 417 2049
SmlI C'TyrA_G
Cuts at: 0 1409 1834 2023 2466
Size: 1409 425 189 443
Fragments arranged by size:
1409 443 425 189
SspI AAT'ATT
Cuts at: 0 1857 2466
Size: 1857 609
StyI C'CwwG_G
Cuts at: 0 500 1383 2466
Size: 500 883 1083
Fragments arranged by size:
1083 883 500
TatI w'GTAC_w
Cuts at: 0 169 769 2466
Size: 169 600 1697
Fragments arranged by size:
1697 600 169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1452 1484 2466
Size: 1452 32 982
Fragments arranged by size:
1452 982 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 2466
Size: 393 2073
XhoI C'TCGA_G
Cuts at: 0 2023 2466
Size: 2023 443
XmnI GAAnn'nnTTC
Cuts at: 0 866 1268 1787 2466
Size: 866 402 519 679
Fragments arranged by size:
866 679 519 402
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApoI AseI AvaI BaeI BanI BanII
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BstAPI
BstEII BstYI BstZ17I Bsu36I BtgI BtsI ClaI DraI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HindIII KpnI MfeI MslI MspA1I NcoI NdeI NsiI
NspI PciI PflMI PpiI PpuMI PsiI PvuII SacI
SapI ScaI SexAI SfcI SmlI SspI StyI TatI
TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI ApaI ApaLI AscI
AsiSI AvrII BamHI BbeI BbvCI BcgI BglI BmtI
BplI BsaAI BsaHI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI
BtgZI CspCI DraIII DrdI EaeI EagI EciI EcoNI
FseI FspI FspAI HaeII HincII HpaI KasI MluI
MmeI MscI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PfoI PmeI PmlI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII Tth111I XbaI XmaI ZraI