Restriction map for
pFN21AB7182
(Linear) (Six-base) MAPSORT of: KIBB7182.seq Check: 3734 from: 1 to: 552
KIBB7182.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 427 552
Size: 427 125
ApaI G_GGCC'C
Cuts at: 0 14 552
Size: 14 538
AvaI C'yCGr_G
Cuts at: 0 223 552
Size: 223 329
BanI G'GyrC_C
Cuts at: 0 238 552
Size: 238 314
BanII G_rGCy'C
Cuts at: 0 14 110 377 508 552
Size: 14 96 267 131 44
Fragments arranged by size:
267 131 96 44 14
BbeI G_GCGC'C
Cuts at: 0 242 552
Size: 242 310
BbsI GAAGACnn'nnnn_
Cuts at: 0 455 552
Size: 455 97
BglI GCCn_nnn'nGGC
Cuts at: 0 26 205 552
Size: 26 179 347
Fragments arranged by size:
347 179 26
Bme1580I G_kGCm'C
Cuts at: 0 14 552
Size: 14 538
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 522 552
Size: 522 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 552
Size: 321 231
Bpu10I CC'TnA_GC
Cuts at: 0 542 552
Size: 542 10
BsaHI Gr'CG_yC
Cuts at: 0 239 365 552
Size: 239 126 187
Fragments arranged by size:
239 187 126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 428 552
Size: 428 124
BseYI C'CCAG_C
Cuts at: 0 252 501 552
Size: 252 249 51
Fragments arranged by size:
252 249 51
BsiEI CG_ry'CG
Cuts at: 0 65 127 137 536 552
Size: 65 62 10 399 16
Fragments arranged by size:
399 65 62 16 10
Bsp1286I G_dGCh'C
Cuts at: 0 14 110 377 508 552
Size: 14 96 267 131 44
Fragments arranged by size:
267 131 96 44 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 86 552
Size: 86 466
BsrFI r'CCGG_y
Cuts at: 0 132 199 535 552
Size: 132 67 336 17
Fragments arranged by size:
336 132 67 17
BstEII G'GTnAC_C
Cuts at: 0 519 552
Size: 519 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 516 552
Size: 501 15 36
Fragments arranged by size:
501 36 15
BtgI C'CryG_G
Cuts at: 0 121 552
Size: 121 431
BtsI GCAGTG_nn'
Cuts at: 0 90 552
Size: 90 462
DraIII CAC_nnn'GTG
Cuts at: 0 96 552
Size: 96 456
DrdI GACnn_nn'nnGTC
Cuts at: 0 82 552
Size: 82 470
EaeI y'GGCC_r
Cuts at: 0 62 124 134 471 533 552
Size: 62 62 10 337 62 19
Fragments arranged by size:
337 62 62 62 19 10
EagI C'GGCC_G
Cuts at: 0 62 124 134 533 552
Size: 62 62 10 399 19
Fragments arranged by size:
399 62 62 19 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 552
Size: 76 476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 552
Size: 321 231
EcoNI CCTnn'n_nnAGG
Cuts at: 0 382 552
Size: 382 170
EcoO109I rG'GnC_Cy
Cuts at: 0 10 11 345 552
Size: 10 1 334 207
Fragments arranged by size:
334 207 10 1
FseI GG_CCGG'CC
Cuts at: 0 539 552
Size: 539 13
FspI TGC'GCA
Cuts at: 0 115 552
Size: 115 437
HaeII r_GCGC'y
Cuts at: 0 169 214 242 277 552
Size: 169 45 28 35 275
Fragments arranged by size:
275 169 45 35 28
KasI G'GCGC_C
Cuts at: 0 238 552
Size: 238 314
MscI TGG'CCA
Cuts at: 0 473 552
Size: 473 79
MspA1I CmG'CkG
Cuts at: 0 123 315 552
Size: 123 192 237
Fragments arranged by size:
237 192 123
NaeI GCC'GGC
Cuts at: 0 201 537 552
Size: 201 336 15
Fragments arranged by size:
336 201 15
NarI GG'CG_CC
Cuts at: 0 239 552
Size: 239 313
NgoMIV G'CCGG_C
Cuts at: 0 199 535 552
Size: 199 336 17
Fragments arranged by size:
336 199 17
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 552
Size: 96 456
PfoI T'CCnGG_A
Cuts at: 0 402 552
Size: 402 150
PspOMI G'GGCC_C
Cuts at: 0 10 552
Size: 10 542
PstI C_TGCA'G
Cuts at: 0 173 552
Size: 173 379
SacII CC_GC'GG
Cuts at: 0 124 552
Size: 124 428
SfcI C'TryA_G
Cuts at: 0 169 552
Size: 169 383
SfoI GGC'GCC
Cuts at: 0 240 552
Size: 240 312
SmaI CCC'GGG
Cuts at: 0 225 552
Size: 225 327
SrfI GCCC'GGGC
Cuts at: 0 225 552
Size: 225 327
Tth111I GACn'n_nGTC
Cuts at: 0 385 552
Size: 385 167
XmaI C'CCGG_G
Cuts at: 0 223 552
Size: 223 329
Enzymes that do cut:
AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI
Bme1580I BplI BpmI Bpu10I BsaHI BseRI BseYI BsiEI
Bsp1286I BspMI BsrFI BstEII BstXI BtgI BtsI DraIII
DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I FseI
FspI HaeII KasI MscI MspA1I NaeI NarI NgoMIV
PflMI PfoI PspOMI PstI SacII SfcI SfoI SmaI
SrfI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI EarI EcoICRI EcoRI EcoRV FalI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MslI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI XbaI XcmI XhoI XmnI
ZraI