Restriction map for
pFN21AB7152
(Linear) (Six-base) MAPSORT of: KIBB7152.seq Check: 8697 from: 1 to: 1053
KIBB7152.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1053
Size: 592 461
AleI CACnn'nnGTG
Cuts at: 0 733 1053
Size: 733 320
AlwNI CAG_nnn'CTG
Cuts at: 0 1036 1053
Size: 1036 17
ApaI G_GGCC'C
Cuts at: 0 399 972 1053
Size: 399 573 81
Fragments arranged by size:
573 399 81
AvaI C'yCGr_G
Cuts at: 0 115 1053
Size: 115 938
BamHI G'GATC_C
Cuts at: 0 77 1053
Size: 77 976
BanI G'GyrC_C
Cuts at: 0 553 790 1053
Size: 553 237 263
Fragments arranged by size:
553 263 237
BanII G_rGCy'C
Cuts at: 0 399 668 865 972 1053
Size: 399 269 197 107 81
Fragments arranged by size:
399 269 197 107 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 330 1053
Size: 330 723
Bme1580I G_kGCm'C
Cuts at: 0 399 793 972 1053
Size: 399 394 179 81
Fragments arranged by size:
399 394 179 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1053
Size: 982 71
BsaWI w'CCGG_w
Cuts at: 0 158 611 1053
Size: 158 453 442
Fragments arranged by size:
453 442 158
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 11 577 1053
Size: 11 566 476
Fragments arranged by size:
566 476 11
BsiHKAI G_wGCw'C
Cuts at: 0 865 1053
Size: 865 188
Bsp1286I G_dGCh'C
Cuts at: 0 399 668 793 865 972 1053
Size: 399 269 125 72 107 81
Fragments arranged by size:
399 269 125 107 81 72
BspEI T'CCGG_A
Cuts at: 0 158 1053
Size: 158 895
BsrDI GCAATG_nn'
Cuts at: 0 135 353 668 1053
Size: 135 218 315 385
Fragments arranged by size:
385 315 218 135
BstEII G'GTnAC_C
Cuts at: 0 102 1053
Size: 102 951
BstXI CCAn_nnnn'nTGG
Cuts at: 0 258 960 1053
Size: 258 702 93
Fragments arranged by size:
702 258 93
BstYI r'GATC_y
Cuts at: 0 77 680 1053
Size: 77 603 373
Fragments arranged by size:
603 373 77
Bsu36I CC'TnA_GG
Cuts at: 0 560 1053
Size: 560 493
BtgI C'CryG_G
Cuts at: 0 251 257 1053
Size: 251 6 796
Fragments arranged by size:
796 251 6
BtsI GCAGTG_nn'
Cuts at: 0 728 1053
Size: 728 325
DraIII CAC_nnn'GTG
Cuts at: 0 309 1053
Size: 309 744
EaeI y'GGCC_r
Cuts at: 0 224 254 422 578 1053
Size: 224 30 168 156 475
Fragments arranged by size:
475 224 168 156 30
EcoICRI GAG'CTC
Cuts at: 0 863 1053
Size: 863 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 982 1053
Size: 592 390 71
Fragments arranged by size:
592 390 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 976 1053
Size: 976 77
EcoO109I rG'GnC_Cy
Cuts at: 0 120 723 968 969 1053
Size: 120 603 245 1 84
Fragments arranged by size:
603 245 120 84 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 441 473 593 625 1053
Size: 441 32 120 32 428
Fragments arranged by size:
441 428 120 32 32
HincII GTy'rAC
Cuts at: 0 852 1053
Size: 852 201
HpaI GTT'AAC
Cuts at: 0 852 1053
Size: 852 201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 1053
Size: 420 633
MscI TGG'CCA
Cuts at: 0 256 424 580 1053
Size: 256 168 156 473
Fragments arranged by size:
473 256 168 156
MslI CAynn'nnrTG
Cuts at: 0 256 310 430 586 733 1053
Size: 256 54 120 156 147 320
Fragments arranged by size:
320 256 156 147 120 54
MspA1I CmG'CkG
Cuts at: 0 368 884 1053
Size: 368 516 169
Fragments arranged by size:
516 368 169
NcoI C'CATG_G
Cuts at: 0 251 257 1053
Size: 251 6 796
Fragments arranged by size:
796 251 6
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 799 831 1053
Size: 799 32 222
Fragments arranged by size:
799 222 32
PpuMI rG'GwC_Cy
Cuts at: 0 120 723 1053
Size: 120 603 330
Fragments arranged by size:
603 330 120
PspOMI G'GGCC_C
Cuts at: 0 395 968 1053
Size: 395 573 85
Fragments arranged by size:
573 395 85
PstI C_TGCA'G
Cuts at: 0 1032 1053
Size: 1032 21
PvuII CAG'CTG
Cuts at: 0 884 1053
Size: 884 169
SacI G_AGCT'C
Cuts at: 0 865 1053
Size: 865 188
SfcI C'TryA_G
Cuts at: 0 494 1028 1053
Size: 494 534 25
Fragments arranged by size:
534 494 25
StyI C'CwwG_G
Cuts at: 0 251 257 445 1053
Size: 251 6 188 608
Fragments arranged by size:
608 251 188 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 1053
Size: 135 918
TatI w'GTAC_w
Cuts at: 0 526 827 1053
Size: 526 301 226
Fragments arranged by size:
526 301 226
Enzymes that do cut:
AcuI AleI AlwNI ApaI AvaI BamHI BanI BanII
BbsI Bme1580I BpmI BsaWI BseRI BsiHKAI Bsp1286I BspEI
BsrDI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII HpaI
MmeI MscI MslI MspA1I NcoI PpiI PpuMI PspOMI
PstI PvuII SacI SfcI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EarI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI