Restriction map for
pFN21AB7131
(Linear) (Six-base) MAPSORT of: KIBB7131.seq Check: 8867 from: 1 to: 951
KIBB7131.orf
With 182 enzymes: SgfI *
March 9, 2016 16:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 951
Size: 926 25
AflII C'TTAA_G
Cuts at: 0 670 951
Size: 670 281
AflIII A'CryG_T
Cuts at: 0 691 951
Size: 691 260
BamHI G'GATC_C
Cuts at: 0 6 951
Size: 6 945
BanI G'GyrC_C
Cuts at: 0 474 951
Size: 474 477
BanII G_rGCy'C
Cuts at: 0 179 950 951
Size: 179 771 1
Fragments arranged by size:
771 179 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 480 892 951
Size: 480 412 59
Fragments arranged by size:
480 412 59
BsaAI yAC'GTr
Cuts at: 0 694 951
Size: 694 257
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 951
Size: 449 502
BsiHKAI G_wGCw'C
Cuts at: 0 103 164 863 951
Size: 103 61 699 88
Fragments arranged by size:
699 103 88 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 732 951
Size: 732 219
Bsp1286I G_dGCh'C
Cuts at: 0 103 164 179 863 950 951
Size: 103 61 15 684 87 1
Fragments arranged by size:
684 103 87 61 15 1
BspHI T'CATG_A
Cuts at: 0 557 951
Size: 557 394
BsrBI CCG'CTC
Cuts at: 0 515 951
Size: 515 436
BsrDI GCAATG_nn'
Cuts at: 0 24 951
Size: 24 927
BssSI C'ACGA_G
Cuts at: 0 422 951
Size: 422 529
BstAPI GCAn_nnn'nTGC
Cuts at: 0 168 951
Size: 168 783
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 630 951
Size: 249 381 321
Fragments arranged by size:
381 321 249
BstYI r'GATC_y
Cuts at: 0 6 951
Size: 6 945
BtgI C'CryG_G
Cuts at: 0 356 779 951
Size: 356 423 172
Fragments arranged by size:
423 356 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 772 951
Size: 772 179
BtsI GCAGTG_nn'
Cuts at: 0 391 951
Size: 391 560
EaeI y'GGCC_r
Cuts at: 0 251 353 377 830 929 951
Size: 251 102 24 453 99 22
Fragments arranged by size:
453 251 102 99 24 22
EarI CTCTTCn'nnn_
Cuts at: 0 62 951
Size: 62 889
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 609 951
Size: 609 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 951
Size: 926 25
EcoRV GAT'ATC
Cuts at: 0 579 951
Size: 579 372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 893 925 951
Size: 893 32 26
Fragments arranged by size:
893 32 26
MfeI C'AATT_G
Cuts at: 0 869 951
Size: 869 82
MscI TGG'CCA
Cuts at: 0 253 355 379 832 931 951
Size: 253 102 24 453 99 20
Fragments arranged by size:
453 253 102 99 24 20
MslI CAynn'nnrTG
Cuts at: 0 551 737 951
Size: 551 186 214
Fragments arranged by size:
551 214 186
MspA1I CmG'CkG
Cuts at: 0 501 816 951
Size: 501 315 135
Fragments arranged by size:
501 315 135
NcoI C'CATG_G
Cuts at: 0 356 779 951
Size: 356 423 172
Fragments arranged by size:
423 356 172
NspI r_CATG'y
Cuts at: 0 201 951
Size: 201 750
PflMI CCAn_nnn'nTGG
Cuts at: 0 260 951
Size: 260 691
PmlI CAC'GTG
Cuts at: 0 694 951
Size: 694 257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 951
Size: 61 32 858
Fragments arranged by size:
858 61 32
PvuII CAG'CTG
Cuts at: 0 501 951
Size: 501 450
ScaI AGT'ACT
Cuts at: 0 410 951
Size: 410 541
SfcI C'TryA_G
Cuts at: 0 301 951
Size: 301 650
SmlI C'TyrA_G
Cuts at: 0 670 951
Size: 670 281
SphI G_CATG'C
Cuts at: 0 201 951
Size: 201 750
StuI AGG'CCT
Cuts at: 0 53 951
Size: 53 898
StyI C'CwwG_G
Cuts at: 0 356 707 779 951
Size: 356 351 72 172
Fragments arranged by size:
356 351 172 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 404 951
Size: 404 547
TatI w'GTAC_w
Cuts at: 0 408 951
Size: 408 543
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 325 348 438 951
Size: 325 23 90 513
Fragments arranged by size:
513 325 90 23
Enzymes that do cut:
AcuI AflII AflIII BamHI BanI BanII BbsI BsaAI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BssSI
BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI
EciI Eco57MI EcoRV Hin4I MfeI MscI MslI MspA1I
NcoI NspI PflMI PmlI PpiI PvuII ScaI SfcI
SmlI SphI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI
BspEI BspMI BsrFI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI