(Linear) (Six-base) MAPSORT of: KIBB7080.seq  Check: 829  from: 1  to: 369



KIBB7080.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:31  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     291     369 
   Size:        291      78
 
ApoI r'AATT_y

Cuts at:      0     346     369 
   Size:        346      23
 
BclI T'GATC_A

Cuts at:      0      50     369 
   Size:         50     319
 
BglI GCCn_nnn'nGGC

Cuts at:      0      74     369 
   Size:         74     295
 
BmgBI CAC'GTC

Cuts at:      0     261     369 
   Size:        261     108
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     369 
   Size:         55     314
 
BspHI T'CATG_A

Cuts at:      0     146     369 
   Size:        146     223
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     104     369 
   Size:        104     265
 
BsrBI CCG'CTC

Cuts at:      0      27     369 
   Size:         27     342
 
BsrDI GCAATG_nn'

Cuts at:      0     237     369 
   Size:        237     132
 
BsrFI r'CCGG_y

Cuts at:      0     338     369 
   Size:        338      31
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     291     369 
   Size:        291      78
 
BstEII G'GTnAC_C

Cuts at:      0      81     369 
   Size:         81     288
 
BtgI C'CryG_G

Cuts at:      0     117     369 
   Size:        117     252
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     322     357     369 
   Size:        322      35      12

  Fragments arranged by size: 

                 322      35      12
 
EaeI y'GGCC_r

Cuts at:      0     161     369 
   Size:        161     208
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     145     369 
   Size:        145     224
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     369 
   Size:         55     314
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      61     369 
   Size:         61     308
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     369 
   Size:         66     303
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     288     320     369 
   Size:        288      32      49

  Fragments arranged by size: 

                 288      49      32
 
MscI TGG'CCA

Cuts at:      0     163     369 
   Size:        163     206
 
MspA1I CmG'CkG

Cuts at:      0     291     369 
   Size:        291      78
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     369 
   Size:        256      32      81

  Fragments arranged by size: 

                 256      81      32
 
PstI C_TGCA'G

Cuts at:      0      65     296     369 
   Size:         65     231      73

  Fragments arranged by size: 

                 231      73      65
 
PvuII CAG'CTG

Cuts at:      0     291     369 
   Size:        291      78
 
RsrII CG'GwC_CG

Cuts at:      0     180     369 
   Size:        180     189
 
SfcI C'TryA_G

Cuts at:      0      61     292     369 
   Size:         61     231      77

  Fragments arranged by size: 

                 231      77      61
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      74     369 
   Size:         74     295
 
StyI C'CwwG_G

Cuts at:      0     333     369 
   Size:        333      36
 
Tth111I GACn'n_nGTC

Cuts at:      0     133     369 
   Size:        133     236
 

 Enzymes that do cut:

    AlwNI     ApoI     BclI     BglI    BmgBI     BpmI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI   BstEII     BtgI    CspCI     EaeI
     EciI  Eco57MI    EcoNI EcoO109I    Hin4I     MscI   MspA1I     PpiI
     PstI    PvuII    RsrII     SfcI     SfiI     StyI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrGI
   BssHII    BssSI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI