Restriction map for
pFN21AB7061
(Linear) (Six-base) MAPSORT of: KIBB7061.seq Check: 1862 from: 1 to: 1965
KIBB7061.orf
With 182 enzymes: SgfI *
February 18, 2014 13:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1309 1965
Size: 1309 656
AfeI AGC'GCT
Cuts at: 0 1825 1965
Size: 1825 140
AflIII A'CryG_T
Cuts at: 0 473 1965
Size: 473 1492
AhdI GACnn_n'nnGTC
Cuts at: 0 1335 1965
Size: 1335 630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1210 1610 1733 1965
Size: 1210 400 123 232
Fragments arranged by size:
1210 400 232 123
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1798 1830 1965
Size: 1798 32 135
Fragments arranged by size:
1798 135 32
ApaI G_GGCC'C
Cuts at: 0 337 1498 1965
Size: 337 1161 467
Fragments arranged by size:
1161 467 337
ApaLI G'TGCA_C
Cuts at: 0 66 1965
Size: 66 1899
ApoI r'AATT_y
Cuts at: 0 630 784 1242 1965
Size: 630 154 458 723
Fragments arranged by size:
723 630 458 154
AvaI C'yCGr_G
Cuts at: 0 382 1647 1965
Size: 382 1265 318
Fragments arranged by size:
1265 382 318
AvrII C'CTAG_G
Cuts at: 0 748 1965
Size: 748 1217
BanI G'GyrC_C
Cuts at: 0 9 126 255 408 1186 1965
Size: 9 117 129 153 778 779
Fragments arranged by size:
779 778 153 129 117 9
BanII G_rGCy'C
Cuts at: 0 337 526 1297 1498 1965
Size: 337 189 771 201 467
Fragments arranged by size:
771 467 337 201 189
BbeI G_GCGC'C
Cuts at: 0 13 130 259 1965
Size: 13 117 129 1706
Fragments arranged by size:
1706 129 117 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1681 1965
Size: 1681 284
BglI GCCn_nnn'nGGC
Cuts at: 0 298 384 1965
Size: 298 86 1581
Fragments arranged by size:
1581 298 86
BglII A'GATC_T
Cuts at: 0 554 1965
Size: 554 1411
BlpI GC'TnA_GC
Cuts at: 0 1583 1965
Size: 1583 382
Bme1580I G_kGCm'C
Cuts at: 0 70 337 413 584 1498 1722 1965
Size: 70 267 76 171 914 224 243
Fragments arranged by size:
914 267 243 224 171 76 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 1073 1167 1965
Size: 1073 94 798
Fragments arranged by size:
1073 798 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 250 814 948 1965
Size: 102 148 564 134 1017
Fragments arranged by size:
1017 564 148 134 102
Bpu10I CC'TnA_GC
Cuts at: 0 220 1965
Size: 220 1745
BsaHI Gr'CG_yC
Cuts at: 0 10 127 158 256 1204 1608 1965
Size: 10 117 31 98 948 404 357
Fragments arranged by size:
948 404 357 117 98 31 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 1400 1725 1965
Size: 247 1153 325 240
Fragments arranged by size:
1153 325 247 240
BseYI C'CCAG_C
Cuts at: 0 357 424 850 1249 1454 1555 1952 1965
Size: 357 67 426 399 205 101 397 13
Fragments arranged by size:
426 399 397 357 205 101 67 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 697 1965
Size: 337 360 1268
Fragments arranged by size:
1268 360 337
BsiEI CG_ry'CG
Cuts at: 0 58 293 302 1965
Size: 58 235 9 1663
Fragments arranged by size:
1663 235 58 9
BsiHKAI G_wGCw'C
Cuts at: 0 70 526 1965
Size: 70 456 1439
Fragments arranged by size:
1439 456 70
BsmI GAATG_Cn'
Cuts at: 0 1029 1965
Size: 1029 936
Bsp1286I G_dGCh'C
Cuts at: 0 70 337 413 526 584 1297 1498 1722
Size: 70 267 76 113 58 713 201 224
Cuts at: 1722 1965
Size: 243
Fragments arranged by size:
713 267 243 224 201 113 76 70
58
BspHI T'CATG_A
Cuts at: 0 209 1487 1965
Size: 209 1278 478
Fragments arranged by size:
1278 478 209
BsrBI CCG'CTC
Cuts at: 0 432 737 1965
Size: 432 305 1228
Fragments arranged by size:
1228 432 305
BsrDI GCAATG_nn'
Cuts at: 0 915 1758 1965
Size: 915 843 207
Fragments arranged by size:
915 843 207
BsrFI r'CCGG_y
Cuts at: 0 405 1965
Size: 405 1560
BsrGI T'GTAC_A
Cuts at: 0 1683 1965
Size: 1683 282
BssSI C'ACGA_G
Cuts at: 0 63 1965
Size: 63 1902
BstYI r'GATC_y
Cuts at: 0 554 1965
Size: 554 1411
Bsu36I CC'TnA_GG
Cuts at: 0 14 1965
Size: 14 1951
BtgI C'CryG_G
Cuts at: 0 161 266 296 767 1965
Size: 161 105 30 471 1198
Fragments arranged by size:
1198 471 161 105 30
DraIII CAC_nnn'GTG
Cuts at: 0 1930 1965
Size: 1930 35
EaeI y'GGCC_r
Cuts at: 0 2 55 73 110 290 299 837 1278
Size: 2 53 18 37 180 9 538 441
Cuts at: 1278 1965
Size: 687
Fragments arranged by size:
687 538 441 180 53 37 18 9
2
EagI C'GGCC_G
Cuts at: 0 55 290 299 1965
Size: 55 235 9 1666
Fragments arranged by size:
1666 235 55 9
EarI CTCTTCn'nnn_
Cuts at: 0 750 1965
Size: 750 1215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 544 1965
Size: 544 1421
EcoICRI GAG'CTC
Cuts at: 0 524 1965
Size: 524 1441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 250 814 948 1309 1965
Size: 102 148 564 134 361 656
Fragments arranged by size:
656 564 361 148 134 102
EcoO109I rG'GnC_Cy
Cuts at: 0 333 1495 1965
Size: 333 1162 470
Fragments arranged by size:
1162 470 333
EcoRI G'AATT_C
Cuts at: 0 630 1242 1965
Size: 630 612 723
Fragments arranged by size:
723 630 612
HaeII r_GCGC'y
Cuts at: 0 13 130 203 259 364 1827 1965
Size: 13 117 73 56 105 1463 138
Fragments arranged by size:
1463 138 117 105 73 56 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 702 734 1083 1115 1321 1353 1828 1860
Size: 702 32 349 32 206 32 475 32
Cuts at: 1860 1965
Size: 105
Fragments arranged by size:
702 475 349 206 105 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1514 1602 1965
Size: 1514 88 363
Fragments arranged by size:
1514 363 88
HindIII A'AGCT_T
Cuts at: 0 1917 1965
Size: 1917 48
HpaI GTT'AAC
Cuts at: 0 1602 1965
Size: 1602 363
KasI G'GCGC_C
Cuts at: 0 9 126 255 1965
Size: 9 117 129 1710
Fragments arranged by size:
1710 129 117 9
MluI A'CGCG_T
Cuts at: 0 473 1965
Size: 473 1492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 1965
Size: 128 1837
MscI TGG'CCA
Cuts at: 0 839 1280 1965
Size: 839 441 685
Fragments arranged by size:
839 685 441
MslI CAynn'nnrTG
Cuts at: 0 673 908 1192 1711 1965
Size: 673 235 284 519 254
Fragments arranged by size:
673 519 284 254 235
MspA1I CmG'CkG
Cuts at: 0 48 78 163 193 298 390 424 1748
Size: 48 30 85 30 105 92 34 1324
Cuts at: 1748 1965
Size: 217
Fragments arranged by size:
1324 217 105 92 85 48 34 30
30
NarI GG'CG_CC
Cuts at: 0 10 127 256 1965
Size: 10 117 129 1709
Fragments arranged by size:
1709 129 117 10
NcoI C'CATG_G
Cuts at: 0 767 1965
Size: 767 1198
NdeI CA'TA_TG
Cuts at: 0 1275 1965
Size: 1275 690
NotI GC'GGCC_GC
Cuts at: 0 290 299 1965
Size: 290 9 1666
Fragments arranged by size:
1666 290 9
NspI r_CATG'y
Cuts at: 0 1582 1815 1965
Size: 1582 233 150
Fragments arranged by size:
1582 233 150
PasI CC'CwG_GG
Cuts at: 0 1722 1965
Size: 1722 243
PflMI CCAn_nnn'nTGG
Cuts at: 0 727 1965
Size: 727 1238
PspOMI G'GGCC_C
Cuts at: 0 333 1494 1965
Size: 333 1161 471
Fragments arranged by size:
1161 471 333
PstI C_TGCA'G
Cuts at: 0 1599 1965
Size: 1599 366
PvuII CAG'CTG
Cuts at: 0 1748 1965
Size: 1748 217
SacI G_AGCT'C
Cuts at: 0 526 1965
Size: 526 1439
SacII CC_GC'GG
Cuts at: 0 164 299 1965
Size: 164 135 1666
Fragments arranged by size:
1666 164 135
SapI GCTCTTCn'nnn_
Cuts at: 0 750 1965
Size: 750 1215
ScaI AGT'ACT
Cuts at: 0 966 1965
Size: 966 999
SfcI C'TryA_G
Cuts at: 0 1595 1965
Size: 1595 370
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 298 1965
Size: 298 1667
SfoI GGC'GCC
Cuts at: 0 11 128 257 1965
Size: 11 117 129 1708
Fragments arranged by size:
1708 129 117 11
SgrAI Cr'CCGG_yG
Cuts at: 0 405 1965
Size: 405 1560
SmaI CCC'GGG
Cuts at: 0 384 1965
Size: 384 1581
SrfI GCCC'GGGC
Cuts at: 0 384 1965
Size: 384 1581
StyI C'CwwG_G
Cuts at: 0 509 748 767 1965
Size: 509 239 19 1198
Fragments arranged by size:
1198 509 239 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 371 1002 1012 1263 1494 1944 1965
Size: 371 631 10 251 231 450 21
Fragments arranged by size:
631 450 371 251 231 21 10
TatI w'GTAC_w
Cuts at: 0 931 964 1683 1965
Size: 931 33 719 282
Fragments arranged by size:
931 719 282 33
Tth111I GACn'n_nGTC
Cuts at: 0 1173 1965
Size: 1173 792
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 847 1180 1570 1965
Size: 847 333 390 395
Fragments arranged by size:
847 395 390 333
XmaI C'CCGG_G
Cuts at: 0 382 1965
Size: 382 1583
XmnI GAAnn'nnTTC
Cuts at: 0 699 1965
Size: 699 1266
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlfI AloI ApaI ApaLI
ApoI AvaI AvrII BanI BanII BbeI BbsI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrDI BsrFI BsrGI BssSI BstYI Bsu36I BtgI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
HaeII Hin4I HincII HindIII HpaI KasI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NotI NspI
PasI PflMI PspOMI PstI PvuII SacI SacII SapI
ScaI SfcI SfiI SfoI SgrAI SmaI SrfI StyI
TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlwNI AscI AseI AsiSI BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BspMI
BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraI DrdI EcoNI EcoRV FalI FseI
FspI FspAI KpnI MfeI NaeI NgoMIV NheI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI SexAI SgfI SmlI SnaBI SpeI SphI SspI
StuI SwaI TstI XbaI XhoI ZraI