Restriction map for
pFN21AB7054
(Linear) (Six-base) MAPSORT of: KIBB7054.seq Check: 8632 from: 1 to: 1722
KIBB7054.orf
With 182 enzymes: SgfI *
July 3, 2012 16:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1426 1722
Size: 1426 296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1061 1722
Size: 1061 661
AflIII A'CryG_T
Cuts at: 0 1426 1483 1722
Size: 1426 57 239
Fragments arranged by size:
1426 239 57
AleI CACnn'nnGTG
Cuts at: 0 1484 1722
Size: 1484 238
AlwNI CAG_nnn'CTG
Cuts at: 0 177 180 793 1000 1722
Size: 177 3 613 207 722
Fragments arranged by size:
722 613 207 177 3
ApaI G_GGCC'C
Cuts at: 0 129 519 1722
Size: 129 390 1203
Fragments arranged by size:
1203 390 129
ApaLI G'TGCA_C
Cuts at: 0 1477 1722
Size: 1477 245
AvaI C'yCGr_G
Cuts at: 0 512 1722
Size: 512 1210
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 419 452 1722
Size: 419 33 1270
Fragments arranged by size:
1270 419 33
BamHI G'GATC_C
Cuts at: 0 936 1722
Size: 936 786
BanI G'GyrC_C
Cuts at: 0 604 774 1722
Size: 604 170 948
Fragments arranged by size:
948 604 170
BanII G_rGCy'C
Cuts at: 0 129 519 1722
Size: 129 390 1203
Fragments arranged by size:
1203 390 129
BbsI GAAGACnn'nnnn_
Cuts at: 0 1199 1352 1441 1722
Size: 1199 153 89 281
Fragments arranged by size:
1199 281 153 89
BbvCI CC'TCA_GC
Cuts at: 0 975 1722
Size: 975 747
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1279 1313 1722
Size: 1279 34 409
Fragments arranged by size:
1279 409 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 420 1722
Size: 420 1302
BglI GCCn_nnn'nGGC
Cuts at: 0 265 647 1722
Size: 265 382 1075
Fragments arranged by size:
1075 382 265
BglII A'GATC_T
Cuts at: 0 64 1722
Size: 64 1658
BlpI GC'TnA_GC
Cuts at: 0 1046 1722
Size: 1046 676
Bme1580I G_kGCm'C
Cuts at: 0 129 519 777 1167 1481 1722
Size: 129 390 258 390 314 241
Fragments arranged by size:
390 390 314 258 241 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 243 654 1722
Size: 243 411 1068
Fragments arranged by size:
1068 411 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 962 995 1013 1166 1234 1364 1722
Size: 683 279 33 18 153 68 130 358
Fragments arranged by size:
683 358 279 153 130 68 33 18
Bpu10I CC'TnA_GC
Cuts at: 0 4 975 1722
Size: 4 971 747
Fragments arranged by size:
971 747 4
BsaI GGTCTCn'nnnn_
Cuts at: 0 823 1722
Size: 823 899
BsaAI yAC'GTr
Cuts at: 0 1486 1722
Size: 1486 236
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 571 601 1139 1169 1722
Size: 187 30 354 30 538 30 553
Fragments arranged by size:
553 538 354 187 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 562 1163 1231 1722
Size: 562 601 68 491
Fragments arranged by size:
601 562 491 68
BseYI C'CCAG_C
Cuts at: 0 369 969 993 1035 1175 1722
Size: 369 600 24 42 140 547
Fragments arranged by size:
600 547 369 140 42 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 1304 1310 1648 1722
Size: 210 1094 6 338 74
Fragments arranged by size:
1094 338 210 74 6
BsiHKAI G_wGCw'C
Cuts at: 0 1481 1722
Size: 1481 241
Bsp1286I G_dGCh'C
Cuts at: 0 129 519 777 1167 1481 1722
Size: 129 390 258 390 314 241
Fragments arranged by size:
390 390 314 258 241 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1426 1722
Size: 1426 296
BsrBI CCG'CTC
Cuts at: 0 315 1722
Size: 315 1407
BstAPI GCAn_nnn'nTGC
Cuts at: 0 180 1722
Size: 180 1542
BstEII G'GTnAC_C
Cuts at: 0 1308 1722
Size: 1308 414
BstYI r'GATC_y
Cuts at: 0 64 333 936 1599 1722
Size: 64 269 603 663 123
Fragments arranged by size:
663 603 269 123 64
Bsu36I CC'TnA_GG
Cuts at: 0 1118 1722
Size: 1118 604
BtgI C'CryG_G
Cuts at: 0 600 1472 1722
Size: 600 872 250
Fragments arranged by size:
872 600 250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 20 1722
Size: 20 1702
BtsI GCAGTG_nn'
Cuts at: 0 883 949 1160 1677 1722
Size: 883 66 211 517 45
Fragments arranged by size:
883 517 211 66 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1410 1445 1722
Size: 1410 35 277
Fragments arranged by size:
1410 277 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1101 1722
Size: 1101 621
EaeI y'GGCC_r
Cuts at: 0 405 1580 1722
Size: 405 1175 142
Fragments arranged by size:
1175 405 142
EarI CTCTTCn'nnn_
Cuts at: 0 858 1085 1722
Size: 858 227 637
Fragments arranged by size:
858 637 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 962 995 1013 1061 1166 1234 1364
Size: 683 279 33 18 48 105 68 130
Cuts at: 1364 1722
Size: 358
Fragments arranged by size:
683 358 279 130 105 68 48 33
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1722
Size: 523 1199
EcoO109I rG'GnC_Cy
Cuts at: 0 114 126 516 904 1123 1539 1575 1612
Size: 114 12 390 388 219 416 36 37
Cuts at: 1612 1722
Size: 110
Fragments arranged by size:
416 390 388 219 114 110 37 36
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 571 603 1302 1334 1722
Size: 187 32 352 32 699 32 388
Fragments arranged by size:
699 388 352 187 32 32 32
HincII GTy'rAC
Cuts at: 0 756 1722
Size: 756 966
HindIII A'AGCT_T
Cuts at: 0 1386 1722
Size: 1386 336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1722
Size: 66 1656
MscI TGG'CCA
Cuts at: 0 407 1582 1722
Size: 407 1175 140
Fragments arranged by size:
1175 407 140
MslI CAynn'nnrTG
Cuts at: 0 232 473 1484 1722
Size: 232 241 1011 238
Fragments arranged by size:
1011 241 238 232
MspA1I CmG'CkG
Cuts at: 0 177 201 411 454 1039 1722
Size: 177 24 210 43 585 683
Fragments arranged by size:
683 585 210 177 43 24
NspI r_CATG'y
Cuts at: 0 1430 1471 1722
Size: 1430 41 251
Fragments arranged by size:
1430 251 41
PasI CC'CwG_GG
Cuts at: 0 207 1198 1722
Size: 207 991 524
Fragments arranged by size:
991 524 207
PciI A'CATG_T
Cuts at: 0 1426 1722
Size: 1426 296
PflMI CCAn_nnn'nTGG
Cuts at: 0 255 376 1722
Size: 255 121 1346
Fragments arranged by size:
1346 255 121
PfoI T'CCnGG_A
Cuts at: 0 191 939 1380 1722
Size: 191 748 441 342
Fragments arranged by size:
748 441 342 191
PmlI CAC'GTG
Cuts at: 0 1486 1722
Size: 1486 236
PpuMI rG'GwC_Cy
Cuts at: 0 114 904 1123 1539 1575 1612 1722
Size: 114 790 219 416 36 37 110
Fragments arranged by size:
790 416 219 114 110 37 36
PspOMI G'GGCC_C
Cuts at: 0 125 515 1722
Size: 125 390 1207
Fragments arranged by size:
1207 390 125
PstI C_TGCA'G
Cuts at: 0 185 286 1722
Size: 185 101 1436
Fragments arranged by size:
1436 185 101
PvuII CAG'CTG
Cuts at: 0 177 411 454 1039 1722
Size: 177 234 43 585 683
Fragments arranged by size:
683 585 234 177 43
SanDI GG'GwC_CC
Cuts at: 0 1612 1722
Size: 1612 110
ScaI AGT'ACT
Cuts at: 0 594 1722
Size: 594 1128
SfcI C'TryA_G
Cuts at: 0 181 282 1722
Size: 181 101 1440
Fragments arranged by size:
1440 181 101
SmaI CCC'GGG
Cuts at: 0 514 1722
Size: 514 1208
SrfI GCCC'GGGC
Cuts at: 0 514 1722
Size: 514 1208
StuI AGG'CCT
Cuts at: 0 49 1722
Size: 49 1673
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 622 1722
Size: 622 1100
TatI w'GTAC_w
Cuts at: 0 592 1722
Size: 592 1130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 325 357 674 706 1139 1171 1722
Size: 325 32 317 32 433 32 551
Fragments arranged by size:
551 433 325 317 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 481 1722
Size: 481 1241
XmaI C'CCGG_G
Cuts at: 0 512 1722
Size: 512 1210
XmnI GAAnn'nnTTC
Cuts at: 0 1438 1722
Size: 1438 284
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApaLI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI
BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI
CspCI DrdI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I
HincII HindIII MmeI MscI MslI MspA1I NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SanDI ScaI SfcI SmaI SrfI StuI TaqII TatI
TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BbeI
BclI BfrBI BmgBI BmtI BplI BpuEI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I ClaI
DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StyI SwaI XbaI XcmI XhoI ZraI