Restriction map for
pFN21AB7037
(Linear) (Six-base) MAPSORT of: KIBB7037.seq Check: 671 from: 1 to: 1560
KIBB7037.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 233 1560
Size: 48 185 1327
Fragments arranged by size:
1327 185 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1560
Size: 988 572
AfeI AGC'GCT
Cuts at: 0 1195 1560
Size: 1195 365
AlwNI CAG_nnn'CTG
Cuts at: 0 40 506 1560
Size: 40 466 1054
Fragments arranged by size:
1054 466 40
ApaI G_GGCC'C
Cuts at: 0 1414 1560
Size: 1414 146
AscI GG'CGCG_CC
Cuts at: 0 1301 1560
Size: 1301 259
AvaI C'yCGr_G
Cuts at: 0 93 1087 1281 1333 1407 1444 1453 1549
Size: 93 994 194 52 74 37 9 96
Cuts at: 1549 1560
Size: 11
Fragments arranged by size:
994 194 96 93 74 52 37 11
9
BamHI G'GATC_C
Cuts at: 0 761 1560
Size: 761 799
BanI G'GyrC_C
Cuts at: 0 157 221 561 1091 1344 1351 1468 1545
Size: 157 64 340 530 253 7 117 77
Cuts at: 1545 1560
Size: 15
Fragments arranged by size:
530 340 253 157 117 77 64 15
7
BanII G_rGCy'C
Cuts at: 0 631 1282 1382 1414 1445 1453 1560
Size: 631 651 100 32 31 8 107
Fragments arranged by size:
651 631 107 100 32 31 8
BbeI G_GCGC'C
Cuts at: 0 161 1348 1355 1472 1549 1560
Size: 161 1187 7 117 77 11
Fragments arranged by size:
1187 161 117 77 11 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 879 913 1560
Size: 879 34 647
Fragments arranged by size:
879 647 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 467 1560
Size: 467 1093
BglI GCCn_nnn'nGGC
Cuts at: 0 490 1145 1244 1394 1504 1560
Size: 490 655 99 150 110 56
Fragments arranged by size:
655 490 150 110 99 56
Bme1580I G_kGCm'C
Cuts at: 0 1414 1560
Size: 1414 146
BmgBI CAC'GTC
Cuts at: 0 1503 1560
Size: 1503 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 57 339 1560
Size: 57 282 1221
Fragments arranged by size:
1221 282 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 1035 1210 1560
Size: 843 192 175 350
Fragments arranged by size:
843 350 192 175
Bpu10I CC'TnA_GC
Cuts at: 0 793 1240 1360 1376 1560
Size: 793 447 120 16 184
Fragments arranged by size:
793 447 184 120 16
BsaHI Gr'CG_yC
Cuts at: 0 158 232 980 1122 1345 1352 1469 1546
Size: 158 74 748 142 223 7 117 77
Cuts at: 1546 1560
Size: 14
Fragments arranged by size:
748 223 158 142 117 77 74 14
7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1033 1063 1560
Size: 1033 30 497
Fragments arranged by size:
1033 497 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 609 1560
Size: 609 951
BseYI C'CCAG_C
Cuts at: 0 113 153 336 1005 1560
Size: 113 40 183 669 555
Fragments arranged by size:
669 555 183 113 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 1560
Size: 369 1191
BsiEI CG_ry'CG
Cuts at: 0 426 1107 1113 1168 1389 1398 1499 1560
Size: 426 681 6 55 221 9 101 61
Fragments arranged by size:
681 426 221 101 61 55 9 6
BsiHKAI G_wGCw'C
Cuts at: 0 323 1560
Size: 323 1237
Bsp1286I G_dGCh'C
Cuts at: 0 323 631 1282 1382 1414 1445 1453 1560
Size: 323 308 651 100 32 31 8 107
Fragments arranged by size:
651 323 308 107 100 32 31 8
BspHI T'CATG_A
Cuts at: 0 722 1560
Size: 722 838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 233 281 1560
Size: 48 185 48 1279
Fragments arranged by size:
1279 185 48 48
BsrBI CCG'CTC
Cuts at: 0 1244 1416 1560
Size: 1244 172 144
Fragments arranged by size:
1244 172 144
BsrFI r'CCGG_y
Cuts at: 0 19 218 558 1163 1560
Size: 19 199 340 605 397
Fragments arranged by size:
605 397 340 199 19
BssHII G'CGCG_C
Cuts at: 0 1068 1301 1560
Size: 1068 233 259
Fragments arranged by size:
1068 259 233
BssSI C'ACGA_G
Cuts at: 0 781 1560
Size: 781 779
BstEII G'GTnAC_C
Cuts at: 0 468 588 1560
Size: 468 120 972
Fragments arranged by size:
972 468 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 1560
Size: 141 1419
BstYI r'GATC_y
Cuts at: 0 344 761 1560
Size: 344 417 799
Fragments arranged by size:
799 417 344
Bsu36I CC'TnA_GG
Cuts at: 0 757 1560
Size: 757 803
BtgI C'CryG_G
Cuts at: 0 263 368 1101 1107 1168 1296 1392 1493
Size: 263 105 733 6 61 128 96 101
Cuts at: 1493 1560
Size: 67
Fragments arranged by size:
733 263 128 105 101 96 67 61
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 918 1560
Size: 918 642
BtsI GCAGTG_nn'
Cuts at: 0 106 1560
Size: 106 1454
ClaI AT'CG_AT
Cuts at: 0 422 1560
Size: 422 1138
EaeI y'GGCC_r
Cuts at: 0 21 260 266 686 1104 1110 1165 1386
Size: 21 239 6 420 418 6 55 221
Cuts at: 1386 1395 1496 1505 1560
Size: 9 101 9 55
Fragments arranged by size:
420 418 239 221 101 55 55 21
9 9 6 6
EagI C'GGCC_G
Cuts at: 0 1104 1110 1165 1386 1395 1496 1560
Size: 1104 6 55 221 9 101 64
Fragments arranged by size:
1104 221 101 64 55 9 6
EarI CTCTTCn'nnn_
Cuts at: 0 619 1423 1560
Size: 619 804 137
Fragments arranged by size:
804 619 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 357 1560
Size: 357 1203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 988 1035 1210 1560
Size: 843 145 47 175 350
Fragments arranged by size:
843 350 175 145 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 504 1560
Size: 504 1056
FspI TGC'GCA
Cuts at: 0 1081 1560
Size: 1081 479
FspAI rTGC'GCAy
Cuts at: 0 1081 1560
Size: 1081 479
HaeII r_GCGC'y
Cuts at: 0 161 496 1197 1348 1355 1472 1549 1560
Size: 161 335 701 151 7 117 77 11
Fragments arranged by size:
701 335 161 151 117 77 11 7
HincII GTy'rAC
Cuts at: 0 255 1560
Size: 255 1305
KasI G'GCGC_C
Cuts at: 0 157 1344 1351 1468 1545 1560
Size: 157 1187 7 117 77 15
Fragments arranged by size:
1187 157 117 77 15 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1560
Size: 761 799
MscI TGG'CCA
Cuts at: 0 262 688 1507 1560
Size: 262 426 819 53
Fragments arranged by size:
819 426 262 53
MslI CAynn'nnrTG
Cuts at: 0 139 436 1560
Size: 139 297 1124
Fragments arranged by size:
1124 297 139
MspA1I CmG'CkG
Cuts at: 0 117 307 1022 1103 1109 1229 1235 1254
Size: 117 190 715 81 6 120 6 19
Cuts at: 1254 1298 1394 1495 1560
Size: 44 96 101 65
Fragments arranged by size:
715 190 120 117 101 96 81 65
44 19 6 6
NaeI GCC'GGC
Cuts at: 0 560 1560
Size: 560 1000
NarI GG'CG_CC
Cuts at: 0 158 1345 1352 1469 1546 1560
Size: 158 1187 7 117 77 14
Fragments arranged by size:
1187 158 117 77 14 7
NcoI C'CATG_G
Cuts at: 0 263 1560
Size: 263 1297
NgoMIV G'CCGG_C
Cuts at: 0 558 1560
Size: 558 1002
NotI GC'GGCC_GC
Cuts at: 0 1104 1110 1395 1560
Size: 1104 6 285 165
Fragments arranged by size:
1104 285 165 6
NspI r_CATG'y
Cuts at: 0 1539 1560
Size: 1539 21
PspOMI G'GGCC_C
Cuts at: 0 1410 1560
Size: 1410 150
PspXI vC'TCGA_Gb
Cuts at: 0 93 1560
Size: 93 1467
PstI C_TGCA'G
Cuts at: 0 455 1560
Size: 455 1105
PvuI CG_AT'CG
Cuts at: 0 426 1560
Size: 426 1134
PvuII CAG'CTG
Cuts at: 0 117 1022 1235 1560
Size: 117 905 213 325
Fragments arranged by size:
905 325 213 117
SacII CC_GC'GG
Cuts at: 0 1104 1110 1299 1395 1496 1560
Size: 1104 6 189 96 101 64
Fragments arranged by size:
1104 189 101 96 64 6
SapI GCTCTTCn'nnn_
Cuts at: 0 1423 1560
Size: 1423 137
SfcI C'TryA_G
Cuts at: 0 451 1560
Size: 451 1109
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1394 1504 1560
Size: 1394 110 56
Fragments arranged by size:
1394 110 56
SfoI GGC'GCC
Cuts at: 0 159 1346 1353 1470 1547 1560
Size: 159 1187 7 117 77 13
Fragments arranged by size:
1187 159 117 77 13 7
SmaI CCC'GGG
Cuts at: 0 1283 1455 1551 1560
Size: 1283 172 96 9
Fragments arranged by size:
1283 172 96 9
SmlI C'TyrA_G
Cuts at: 0 93 1560
Size: 93 1467
SrfI GCCC'GGGC
Cuts at: 0 1283 1551 1560
Size: 1283 268 9
Fragments arranged by size:
1283 268 9
StyI C'CwwG_G
Cuts at: 0 263 543 743 803 809 1560
Size: 263 280 200 60 6 751
Fragments arranged by size:
751 280 263 200 60 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 321 536 579 1453 1474 1560
Size: 321 215 43 874 21 86
Fragments arranged by size:
874 321 215 86 43 21
TatI w'GTAC_w
Cuts at: 0 665 1560
Size: 665 895
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 336 364 368 396 1560
Size: 336 28 4 28 1164
Fragments arranged by size:
1164 336 28 28 4
XhoI C'TCGA_G
Cuts at: 0 93 1560
Size: 93 1467
XmaI C'CCGG_G
Cuts at: 0 1281 1453 1549 1560
Size: 1281 172 96 11
Fragments arranged by size:
1281 172 96 11
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AscI AvaI BamHI
BanI BanII BbeI BcgI BciVI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI EaeI EagI EarI EciI Eco57MI FalI FspI
FspAI HaeII HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PspOMI PspXI
PstI PvuI PvuII SacII SapI SfcI SfiI SfoI
SmaI SmlI SrfI StyI TaqII TatI TstI XhoI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII
BaeI BbsI BbvCI BclI BfrBI BglII BlpI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmI
BsmBI BspEI BsrDI BsrGI BstAPI BstBI BstZ17I CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI Hin4I HindIII HpaI KpnI MfeI MluI NdeI
NheI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PsrI RsrII
SacI SalI SanDI SbfI ScaI SexAI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI
XcmI XmnI ZraI