(Linear) (Six-base) MAPSORT of: KIBB6993.seq  Check: 410  from: 1  to: 663



KIBB6993.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     215     663 
   Size:        215     448
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     605     663 
   Size:        249     356      58

  Fragments arranged by size: 

                 356     249      58
 
AleI CACnn'nnGTG

Cuts at:      0     121     663 
   Size:        121     542
 
AlwNI CAG_nnn'CTG

Cuts at:      0     335     663 
   Size:        335     328
 
ApoI r'AATT_y

Cuts at:      0      97     109     663 
   Size:         97      12     554

  Fragments arranged by size: 

                 554      97      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      70     663 
   Size:         70     593
 
BfrBI ATG'CAT

Cuts at:      0     357     361     663 
   Size:        357       4     302

  Fragments arranged by size: 

                 357     302       4
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     663 
   Size:        430     233
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     448     663 
   Size:        448     215
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     215     663 
   Size:        215     448
 
BsrGI T'GTAC_A

Cuts at:      0     155     663 
   Size:        155     508
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     430     605     663 
   Size:        249     181     175      58

  Fragments arranged by size: 

                 249     181     175      58
 
MfeI C'AATT_G

Cuts at:      0     518     663 
   Size:        518     145
 
MslI CAynn'nnrTG

Cuts at:      0     121     663 
   Size:        121     542
 
NsiI A_TGCA'T

Cuts at:      0     359     363     663 
   Size:        359       4     300

  Fragments arranged by size: 

                 359     300       4
 
NspI r_CATG'y

Cuts at:      0     361     656     663 
   Size:        361     295       7

  Fragments arranged by size: 

                 361     295       7
 
PfoI T'CCnGG_A

Cuts at:      0     574     663 
   Size:        574      89
 
PstI C_TGCA'G

Cuts at:      0     212     663 
   Size:        212     451
 
SfcI C'TryA_G

Cuts at:      0     208     349     663 
   Size:        208     141     314

  Fragments arranged by size: 

                 314     208     141
 
SpeI A'CTAG_T

Cuts at:      0     484     663 
   Size:        484     179
 
SphI G_CATG'C

Cuts at:      0     361     663 
   Size:        361     302
 
TatI w'GTAC_w

Cuts at:      0      72     155     663 
   Size:         72      83     508

  Fragments arranged by size: 

                 508      83      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      54      86     663 
   Size:         54      32     577

  Fragments arranged by size: 

                 577      54      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      88     663 
   Size:         88     575
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApoI     BbsI    BfrBI     BpmI
    BseRI    BspMI    BsrGI  Eco57MI     MfeI     MslI     NsiI     NspI
     PfoI     PstI     SfcI     SpeI     SphI     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI