(Linear) (Six-base) MAPSORT of: KIBB6914.seq  Check: 405  from: 1  to: 675



KIBB6914.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     247     675 
   Size:        203      44     428

  Fragments arranged by size: 

                 428     203      44
 
AhdI GACnn_n'nnGTC

Cuts at:      0     253     675 
   Size:        253     422
 
AlwNI CAG_nnn'CTG

Cuts at:      0     307     658     675 
   Size:        307     351      17

  Fragments arranged by size: 

                 351     307      17
 
ApoI r'AATT_y

Cuts at:      0     167     648     675 
   Size:        167     481      27

  Fragments arranged by size: 

                 481     167      27
 
BanI G'GyrC_C

Cuts at:      0      46     675 
   Size:         46     629
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     394     675 
   Size:        394     281
 
BbvCI CC'TCA_GC

Cuts at:      0      28     675 
   Size:         28     647
 
Bme1580I G_kGCm'C

Cuts at:      0      49     675 
   Size:         49     626
 
Bpu10I CC'TnA_GC

Cuts at:      0      28     675 
   Size:         28     647
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     675 
   Size:        256     419
 
BseYI C'CCAG_C

Cuts at:      0     427     675 
   Size:        427     248
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     675 
   Size:        350     325
 
BsmI GAATG_Cn'

Cuts at:      0     277     675 
   Size:        277     398
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     675 
   Size:         49     626
 
BtsI GCAGTG_nn'

Cuts at:      0      29     675 
   Size:         29     646
 
EarI CTCTTCn'nnn_

Cuts at:      0     629     675 
   Size:        629      46
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      25      48     675 
   Size:         25      23     627

  Fragments arranged by size: 

                 627      25      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     247     675 
   Size:        203      44     428

  Fragments arranged by size: 

                 428     203      44
 
EcoRI G'AATT_C

Cuts at:      0     648     675 
   Size:        648      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     239     271     675 
   Size:        239      32     404

  Fragments arranged by size: 

                 404     239      32
 
MslI CAynn'nnrTG

Cuts at:      0     295     558     675 
   Size:        295     263     117

  Fragments arranged by size: 

                 295     263     117
 
NspI r_CATG'y

Cuts at:      0     401     508     675 
   Size:        401     107     167

  Fragments arranged by size: 

                 401     167     107
 
SfcI C'TryA_G

Cuts at:      0     362     455     675 
   Size:        362      93     220

  Fragments arranged by size: 

                 362     220      93
 
SmlI C'TyrA_G

Cuts at:      0     235     675 
   Size:        235     440
 
SspI AAT'ATT

Cuts at:      0     159     421     497     675 
   Size:        159     262      76     178

  Fragments arranged by size: 

                 262     178     159      76
 
StyI C'CwwG_G

Cuts at:      0      50     605     675 
   Size:         50     555      70

  Fragments arranged by size: 

                 555      70      50
 
TatI w'GTAC_w

Cuts at:      0     103     642     675 
   Size:        103     539      33

  Fragments arranged by size: 

                 539     103      33
 
XmnI GAAnn'nnTTC

Cuts at:      0     498     675 
   Size:        498     177
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApoI     BanI     BbsI    BbvCI Bme1580I
   Bpu10I    BpuEI    BseYI     BsgI     BsmI Bsp1286I     BtsI     EarI
     EciI  Eco57MI    EcoRI    Hin4I     MslI     NspI     SfcI     SmlI
     SspI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI