(Linear) (Six-base) MAPSORT of: KIBB6827.seq  Check: 911  from: 1  to: 498



KIBB6827.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:30  ..

AflII C'TTAA_G

Cuts at:      0     100     498 
   Size:        100     398
 
AlwNI CAG_nnn'CTG

Cuts at:      0     471     498 
   Size:        471      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     299     498 
   Size:        299     199
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     274     498 
   Size:        274     224
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     196     498 
   Size:        196     302
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     109     498 
   Size:        109     389
 
BstBI TT'CG_AA

Cuts at:      0     344     498 
   Size:        344     154
 
DraI TTT'AAA

Cuts at:      0     240     249     498 
   Size:        240       9     249

  Fragments arranged by size: 

                 249     240       9
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     301     379     498 
   Size:        301      78     119

  Fragments arranged by size: 

                 301     119      78
 
EarI CTCTTCn'nnn_

Cuts at:      0     480     498 
   Size:        480      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     498 
   Size:         72     426
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     408     498 
   Size:        408      90
 
MslI CAynn'nnrTG

Cuts at:      0     128     412     498 
   Size:        128     284      86

  Fragments arranged by size: 

                 284     128      86
 
NdeI CA'TA_TG

Cuts at:      0      78     498 
   Size:         78     420
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     417     498 
   Size:        417      81
 
PpuMI rG'GwC_Cy

Cuts at:      0      72     498 
   Size:         72     426
 
SapI GCTCTTCn'nnn_

Cuts at:      0     480     498 
   Size:        480      18
 
SmlI C'TyrA_G

Cuts at:      0     100     498 
   Size:        100     398
 
SspI AAT'ATT

Cuts at:      0     109     498 
   Size:        109     389
 

 Enzymes that do cut:

    AflII    AlwNI     BbsI     BsaI    BseRI    BspMI    BstBI     DraI
     DrdI     EarI EcoO109I     MmeI     MslI     NdeI    PflMI    PpuMI
     SapI     SmlI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI