(Linear) (Six-base) MAPSORT of: KIBB6817.seq  Check: 5726  from: 1  to: 486



KIBB6817.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     486 
   Size:        328     158
 
AleI CACnn'nnGTG

Cuts at:      0     473     486 
   Size:        473      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0     453     486 
   Size:        453      33
 
ApoI r'AATT_y

Cuts at:      0     175     403     486 
   Size:        175     228      83

  Fragments arranged by size: 

                 228     175      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     348     486 
   Size:        348     138
 
BclI T'GATC_A

Cuts at:      0     138     486 
   Size:        138     348
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     486 
   Size:        173     313
 
BsaBI GATnn'nnATC

Cuts at:      0      34      49     420     486 
   Size:         34      15     371      66

  Fragments arranged by size: 

                 371      66      34      15
 
BsmI GAATG_Cn'

Cuts at:      0     119     486 
   Size:        119     367
 
BstYI r'GATC_y

Cuts at:      0       4     486 
   Size:          4     482
 
EarI CTCTTCn'nnn_

Cuts at:      0     304     486 
   Size:        304     182
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     328     486 
   Size:        173     155     158

  Fragments arranged by size: 

                 173     158     155
 
EcoRI G'AATT_C

Cuts at:      0     403     486 
   Size:        403      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     429     461     486 
   Size:         26     403      32      25

  Fragments arranged by size: 

                 403      32      26      25
 
HincII GTy'rAC

Cuts at:      0     121     486 
   Size:        121     365
 
MslI CAynn'nnrTG

Cuts at:      0     473     486 
   Size:        473      13
 
MspA1I CmG'CkG

Cuts at:      0     144     486 
   Size:        144     342
 
NspI r_CATG'y

Cuts at:      0     365     486 
   Size:        365     121
 
PshAI GACnn'nnGTC

Cuts at:      0      67     486 
   Size:         67     419
 
PvuII CAG'CTG

Cuts at:      0     144     486 
   Size:        144     342
 
StyI C'CwwG_G

Cuts at:      0      87     486 
   Size:         87     399
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     411     486 
   Size:        411      75
 
Tth111I GACn'n_nGTC

Cuts at:      0     166     486 
   Size:        166     320
 
XbaI T'CTAG_A

Cuts at:      0     460     486 
   Size:        460      26
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI     BbsI     BclI     BpmI    BsaBI
     BsmI    BstYI     EarI  Eco57MI    EcoRI    Hin4I   HincII     MslI
   MspA1I     NspI    PshAI    PvuII     StyI    TaqII  Tth111I     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI     XcmI
     XhoI     XmaI     XmnI     ZraI