(Linear) (Six-base) MAPSORT of: KIBB6784.seq  Check: 3151  from: 1  to: 459



KIBB6784.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:35  ..

ApaLI G'TGCA_C

Cuts at:      0     141     459 
   Size:        141     318
 
ApoI r'AATT_y

Cuts at:      0     360     459 
   Size:        360      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     139     459 
   Size:        139     320
 
BglI GCCn_nnn'nGGC

Cuts at:      0     353     459 
   Size:        353     106
 
Bme1580I G_kGCm'C

Cuts at:      0     145     459 
   Size:        145     314
 
BmtI G_CTAG'C

Cuts at:      0      46     459 
   Size:         46     413
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      10     459 
   Size:         10     449
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     459 
   Size:        263     196
 
BsiHKAI G_wGCw'C

Cuts at:      0     145     227     459 
   Size:        145      82     232

  Fragments arranged by size: 

                 232     145      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     145     227     459 
   Size:        145      82     232

  Fragments arranged by size: 

                 232     145      82
 
BspHI T'CATG_A

Cuts at:      0     428     459 
   Size:        428      31
 
BsrFI r'CCGG_y

Cuts at:      0      52     459 
   Size:         52     407
 
BtgI C'CryG_G

Cuts at:      0      66     459 
   Size:         66     393
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     207     459 
   Size:        207     252
 
EcoO109I rG'GnC_Cy

Cuts at:      0     345     459 
   Size:        345     114
 
EcoRI G'AATT_C

Cuts at:      0     360     459 
   Size:        360      99
 
EcoRV GAT'ATC

Cuts at:      0      84     459 
   Size:         84     375
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     219     459 
   Size:        219     240
 
HincII GTy'rAC

Cuts at:      0     152     459 
   Size:        152     307
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     459 
   Size:        377      82
 
MspA1I CmG'CkG

Cuts at:      0     306     459 
   Size:        306     153
 
NaeI GCC'GGC

Cuts at:      0      54     459 
   Size:         54     405
 
NgoMIV G'CCGG_C

Cuts at:      0      52     459 
   Size:         52     407
 
NheI G'CTAG_C

Cuts at:      0      42     459 
   Size:         42     417
 
NspI r_CATG'y

Cuts at:      0      50     459 
   Size:         50     409
 
PstI C_TGCA'G

Cuts at:      0     211     459 
   Size:        211     248
 
PvuII CAG'CTG

Cuts at:      0     306     459 
   Size:        306     153
 
SfcI C'TryA_G

Cuts at:      0     207     237     459 
   Size:        207      30     222

  Fragments arranged by size: 

                 222     207      30
 
SspI AAT'ATT

Cuts at:      0     442     459 
   Size:        442      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     259     459 
   Size:        259     200
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     272     304     459 
   Size:        272      32     155

  Fragments arranged by size: 

                 272     155      32
 

 Enzymes that do cut:

    ApaLI     ApoI     BbsI     BglI Bme1580I     BmtI     BsaI     BsgI
  BsiHKAI Bsp1286I    BspHI    BsrFI     BtgI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI   HincII     MmeI   MspA1I     NaeI   NgoMIV     NheI
     NspI     PstI    PvuII     SfcI     SspI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NarI     NcoI     NdeI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI