(Linear) (Six-base) MAPSORT of: KIBB6778.seq  Check: 8551  from: 1  to: 456



KIBB6778.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:35  ..

ApoI r'AATT_y

Cuts at:      0     373     456 
   Size:        373      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      91     191     456 
   Size:         91     100     265

  Fragments arranged by size: 

                 265     100      91
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     456 
   Size:        250     206
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     456 
   Size:        430      26
 
BsaWI w'CCGG_w

Cuts at:      0     377     456 
   Size:        377      79
 
BseYI C'CCAG_C

Cuts at:      0      92     456 
   Size:         92     364
 
BsiEI CG_ry'CG

Cuts at:      0     326     456 
   Size:        326     130
 
BsmI GAATG_Cn'

Cuts at:      0     235     284     420     456 
   Size:        235      49     136      36

  Fragments arranged by size: 

                 235     136      49      36
 
BspEI T'CCGG_A

Cuts at:      0     377     456 
   Size:        377      79
 
BstBI TT'CG_AA

Cuts at:      0     216     456 
   Size:        216     240
 
BtgI C'CryG_G

Cuts at:      0     320     456 
   Size:        320     136
 
ClaI AT'CG_AT

Cuts at:      0     116     456 
   Size:        116     340
 
EaeI y'GGCC_r

Cuts at:      0     317     323     456 
   Size:        317       6     133

  Fragments arranged by size: 

                 317     133       6
 
EagI C'GGCC_G

Cuts at:      0     323     456 
   Size:        323     133
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      31     456 
   Size:         31     425
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     456 
   Size:        250     206
 
EcoRI G'AATT_C

Cuts at:      0     373     456 
   Size:        373      83
 
FspI TGC'GCA

Cuts at:      0     419     456 
   Size:        419      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     329     361     381     413     456 
   Size:        329      32      20      32      43

  Fragments arranged by size: 

                 329      43      32      32      20
 
HincII GTy'rAC

Cuts at:      0      99     456 
   Size:         99     357
 
HpaI GTT'AAC

Cuts at:      0      99     456 
   Size:         99     357
 
MfeI C'AATT_G

Cuts at:      0     275     456 
   Size:        275     181
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     456 
   Size:         54     402
 
MscI TGG'CCA

Cuts at:      0     319     456 
   Size:        319     137
 
MslI CAynn'nnrTG

Cuts at:      0     199     456 
   Size:        199     257
 
NruI TCG'CGA

Cuts at:      0     448     456 
   Size:        448       8
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      36     456 
   Size:         36     420
 
PsiI TTA'TAA

Cuts at:      0     127     456 
   Size:        127     329
 
SmlI C'TyrA_G

Cuts at:      0     409     456 
   Size:        409      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     345     456 
   Size:        345     111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     324     356     456 
   Size:        324      32     100

  Fragments arranged by size: 

                 324     100      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     373     456 
   Size:        373      83
 

 Enzymes that do cut:

     ApoI     BbsI     BpmI    BpuEI    BsaWI    BseYI    BsiEI     BsmI
    BspEI    BstBI     BtgI     ClaI     EaeI     EagI     EciI  Eco57MI
    EcoRI     FspI    Hin4I   HincII     HpaI     MfeI     MmeI     MscI
     MslI     NruI    PflMI     PsiI     SmlI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EarI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
    HaeII  HindIII     KasI     KpnI     MluI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI