(Linear) (Six-base) MAPSORT of: KIBB6744.seq  Check: 63  from: 1  to: 432



KIBB6744.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     394     432 
   Size:        189     205      38

  Fragments arranged by size: 

                 205     189      38
 
AhdI GACnn_n'nnGTC

Cuts at:      0     158     432 
   Size:        158     274
 
AleI CACnn'nnGTG

Cuts at:      0     140     432 
   Size:        140     292
 
ApoI r'AATT_y

Cuts at:      0     389     432 
   Size:        389      43
 
BamHI G'GATC_C

Cuts at:      0      77     432 
   Size:         77     355
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     373     432 
   Size:        373      59
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     236     432 
   Size:        236     196
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     174     432 
   Size:        174     258
 
BsiHKAI G_wGCw'C

Cuts at:      0      43     432 
   Size:         43     389
 
BsmI GAATG_Cn'

Cuts at:      0     173     432 
   Size:        173     259
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     432 
   Size:         43     389
 
BstYI r'GATC_y

Cuts at:      0      77     117     432 
   Size:         77      40     315

  Fragments arranged by size: 

                 315      77      40
 
BtgI C'CryG_G

Cuts at:      0      11      44     347     432 
   Size:         11      33     303      85

  Fragments arranged by size: 

                 303      85      33      11
 
BtsI GCAGTG_nn'

Cuts at:      0     135     147     432 
   Size:        135      12     285

  Fragments arranged by size: 

                 285     135      12
 
EaeI y'GGCC_r

Cuts at:      0       8     432 
   Size:          8     424
 
EarI CTCTTCn'nnn_

Cuts at:      0     370     432 
   Size:        370      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     394     432 
   Size:        189     205      38

  Fragments arranged by size: 

                 205     189      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     144     176     202     234     252     284     432 
   Size:        144      32      26      32      18      32     148

  Fragments arranged by size: 

                 148     144      32      32      32      26      18
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     432 
   Size:         88     344
 
MscI TGG'CCA

Cuts at:      0      10     432 
   Size:         10     422
 
MslI CAynn'nnrTG

Cuts at:      0     140     432 
   Size:        140     292
 
NcoI C'CATG_G

Cuts at:      0      11     432 
   Size:         11     421
 
PfoI T'CCnGG_A

Cuts at:      0      80     432 
   Size:         80     352
 
PstI C_TGCA'G

Cuts at:      0     142     432 
   Size:        142     290
 
ScaI AGT'ACT

Cuts at:      0     293     432 
   Size:        293     139
 
SfcI C'TryA_G

Cuts at:      0     138     432 
   Size:        138     294
 
StyI C'CwwG_G

Cuts at:      0      11      68     432 
   Size:         11      57     364

  Fragments arranged by size: 

                 364      57      11
 
TatI w'GTAC_w

Cuts at:      0     291     432 
   Size:        291     141
 

 Enzymes that do cut:

     AcuI     AhdI     AleI     ApoI    BamHI     BbsI     BplI     BsgI
  BsiHKAI     BsmI Bsp1286I    BstYI     BtgI     BtsI     EaeI     EarI
  Eco57MI    Hin4I     MmeI     MscI     MslI     NcoI     PfoI     PstI
     ScaI     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI