Restriction map for
pFN21AB6736
(Linear) (Six-base) MAPSORT of: KIBB6736.seq Check: 4490 from: 1 to: 417
KIBB6736.orf
With 182 enzymes: SgfI *
March 22, 2012 15:35 ..
AccI GT'mk_AC
Cuts at: 0 411 417
Size: 411 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 417
Size: 285 132
AleI CACnn'nnGTG
Cuts at: 0 405 417
Size: 405 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 247 417
Size: 247 170
BanI G'GyrC_C
Cuts at: 0 46 91 417
Size: 46 45 326
Fragments arranged by size:
326 46 45
BanII G_rGCy'C
Cuts at: 0 158 417
Size: 158 259
BbeI G_GCGC'C
Cuts at: 0 95 417
Size: 95 322
BglI GCCn_nnn'nGGC
Cuts at: 0 207 417
Size: 207 210
Bme1580I G_kGCm'C
Cuts at: 0 49 417
Size: 49 368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 417
Size: 27 390
BsaBI GATnn'nnATC
Cuts at: 0 169 417
Size: 169 248
BsaHI Gr'CG_yC
Cuts at: 0 92 417
Size: 92 325
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 115 417
Size: 112 3 302
Fragments arranged by size:
302 112 3
BsiHKAI G_wGCw'C
Cuts at: 0 158 417
Size: 158 259
Bsp1286I G_dGCh'C
Cuts at: 0 49 158 417
Size: 49 109 259
Fragments arranged by size:
259 109 49
BsrBI CCG'CTC
Cuts at: 0 13 417
Size: 13 404
BstZ17I GTA'TAC
Cuts at: 0 412 417
Size: 412 5
BtsI GCAGTG_nn'
Cuts at: 0 400 412 417
Size: 400 12 5
Fragments arranged by size:
400 12 5
EarI CTCTTCn'nnn_
Cuts at: 0 62 77 304 417
Size: 62 15 227 113
Fragments arranged by size:
227 113 62 15
EcoICRI GAG'CTC
Cuts at: 0 156 417
Size: 156 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 285 417
Size: 27 258 132
Fragments arranged by size:
258 132 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 308 417
Size: 308 109
HaeII r_GCGC'y
Cuts at: 0 95 417
Size: 95 322
HincII GTy'rAC
Cuts at: 0 336 417
Size: 336 81
HindIII A'AGCT_T
Cuts at: 0 325 417
Size: 325 92
HpaI GTT'AAC
Cuts at: 0 336 417
Size: 336 81
KasI G'GCGC_C
Cuts at: 0 91 417
Size: 91 326
MslI CAynn'nnrTG
Cuts at: 0 405 417
Size: 405 12
NarI GG'CG_CC
Cuts at: 0 92 417
Size: 92 325
PfoI T'CCnGG_A
Cuts at: 0 160 417
Size: 160 257
PstI C_TGCA'G
Cuts at: 0 407 417
Size: 407 10
SacI G_AGCT'C
Cuts at: 0 158 417
Size: 158 259
SapI GCTCTTCn'nnn_
Cuts at: 0 304 417
Size: 304 113
ScaI AGT'ACT
Cuts at: 0 73 417
Size: 73 344
SfcI C'TryA_G
Cuts at: 0 403 417
Size: 403 14
SfoI GGC'GCC
Cuts at: 0 93 417
Size: 93 324
StyI C'CwwG_G
Cuts at: 0 292 417
Size: 292 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 417
Size: 64 353
TatI w'GTAC_w
Cuts at: 0 71 417
Size: 71 346
Enzymes that do cut:
AccI AcuI AleI AlfI BanI BanII BbeI BglI
Bme1580I BpmI BsaBI BsaHI BseRI BsiHKAI Bsp1286I BsrBI
BstZ17I BtsI EarI EcoICRI Eco57MI FalI HaeII HincII
HindIII HpaI KasI MslI NarI PfoI PstI SacI
SapI ScaI SfcI SfoI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI Hin4I KpnI MfeI MluI
MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI