(Linear) (Six-base) MAPSORT of: KIBB6641.seq  Check: 7764  from: 1  to: 1254



KIBB6641.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     783    1254 
   Size:        783     471
 
AccI GT'mk_AC

Cuts at:      0     923    1254 
   Size:        923     331
 
AclI AA'CG_TT

Cuts at:      0     161    1254 
   Size:        161    1093
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479    1254 
   Size:        479     775
 
AleI CACnn'nnGTG

Cuts at:      0     682    1254 
   Size:        682     572
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     700    1254 
   Size:        700     554
 
AlwNI CAG_nnn'CTG

Cuts at:      0     828     840    1254 
   Size:        828      12     414

  Fragments arranged by size: 

                 828     414      12
 
ApoI r'AATT_y

Cuts at:      0     906    1249    1254 
   Size:        906     343       5

  Fragments arranged by size: 

                 906     343       5
 
BanI G'GyrC_C

Cuts at:      0     795    1254 
   Size:        795     459
 
BanII G_rGCy'C

Cuts at:      0     179    1254 
   Size:        179    1075
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      88    1254 
   Size:         88    1166
 
BfrBI ATG'CAT

Cuts at:      0     813    1254 
   Size:        813     441
 
BmgBI CAC'GTC

Cuts at:      0     121    1254 
   Size:        121    1133
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     906    1254 
   Size:        906     348
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     939    1172    1254 
   Size:        939     233      82

  Fragments arranged by size: 

                 939     233      82
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     462    1254 
   Size:        462     792
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     929     959     999    1029    1254 
   Size:        929      30      40      30     225

  Fragments arranged by size: 

                 929     225      40      30      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     508     706    1254 
   Size:          6     502     198     548

  Fragments arranged by size: 

                 548     502     198       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     179    1254 
   Size:        179    1075
 
Bsp1286I G_dGCh'C

Cuts at:      0     179    1254 
   Size:        179    1075
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     783    1254 
   Size:        783     471
 
BsrDI GCAATG_nn'

Cuts at:      0     294    1180    1254 
   Size:        294     886      74

  Fragments arranged by size: 

                 886     294      74
 
BstYI r'GATC_y

Cuts at:      0    1056    1254 
   Size:       1056     198
 
BtgI C'CryG_G

Cuts at:      0     242     691    1254 
   Size:        242     449     563

  Fragments arranged by size: 

                 563     449     242
 
EarI CTCTTCn'nnn_

Cuts at:      0     189    1254 
   Size:        189    1065
 
EcoICRI GAG'CTC

Cuts at:      0     177    1254 
   Size:        177    1077
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     939    1172    1254 
   Size:        479     460     233      82

  Fragments arranged by size: 

                 479     460     233      82
 
EcoO109I rG'GnC_Cy

Cuts at:      0     446     847    1254 
   Size:        446     401     407

  Fragments arranged by size: 

                 446     407     401
 
EcoRI G'AATT_C

Cuts at:      0    1249    1254 
   Size:       1249       5
 
EcoRV GAT'ATC

Cuts at:      0     870    1254 
   Size:        870     384
 
HincII GTy'rAC

Cuts at:      0     436    1254 
   Size:        436     818
 
HindIII A'AGCT_T

Cuts at:      0     305    1254 
   Size:        305     949
 
MslI CAynn'nnrTG

Cuts at:      0     530     682    1254 
   Size:        530     152     572

  Fragments arranged by size: 

                 572     530     152
 
MspA1I CmG'CkG

Cuts at:      0     389    1254 
   Size:        389     865
 
NsiI A_TGCA'T

Cuts at:      0     815    1254 
   Size:        815     439
 
NspI r_CATG'y

Cuts at:      0     776     813    1254 
   Size:        776      37     441

  Fragments arranged by size: 

                 776     441      37
 
PfoI T'CCnGG_A

Cuts at:      0     803    1254 
   Size:        803     451
 
PpuMI rG'GwC_Cy

Cuts at:      0     446     847    1254 
   Size:        446     401     407

  Fragments arranged by size: 

                 446     407     401
 
PshAI GACnn'nnGTC

Cuts at:      0     217    1254 
   Size:        217    1037
 
PsiI TTA'TAA

Cuts at:      0    1163    1254 
   Size:       1163      91
 
PvuII CAG'CTG

Cuts at:      0     389    1254 
   Size:        389     865
 
SacI G_AGCT'C

Cuts at:      0     179    1254 
   Size:        179    1075
 
ScaI AGT'ACT

Cuts at:      0     146    1254 
   Size:        146    1108
 
SmlI C'TyrA_G

Cuts at:      0     441    1254 
   Size:        441     813
 
SspI AAT'ATT

Cuts at:      0     354    1254 
   Size:        354     900
 
StyI C'CwwG_G

Cuts at:      0    1198    1254 
   Size:       1198      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     998    1254 
   Size:        998     256
 
TatI w'GTAC_w

Cuts at:      0     144    1254 
   Size:        144    1110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     929     961     982    1014    1254 
   Size:        929      32      21      32     240

  Fragments arranged by size: 

                 929     240      32      32      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     110     249    1196    1254 
   Size:        110     139     947      58

  Fragments arranged by size: 

                 947     139     110      58
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AleI     AlfI    AlwNI     ApoI
     BanI    BanII    BciVI    BfrBI    BmgBI     BmrI     BpmI    BpuEI
    BsaXI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI    BstYI     BtgI
     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV   HincII  HindIII
     MslI   MspA1I     NsiI     NspI     PfoI    PpuMI    PshAI     PsiI
    PvuII     SacI     ScaI     SmlI     SspI     StyI    TaqII     TatI
     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII
     BlpI Bme1580I     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI
     FalI     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI