Restriction map for
pFN21AB6540
(Linear) (Six-base) MAPSORT of: KIBB6540.seq Check: 1157 from: 1 to: 1173
KIBB6540.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 230 909 1173
Size: 76 154 679 264
Fragments arranged by size:
679 264 154 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 751 1173
Size: 751 422
AlwNI CAG_nnn'CTG
Cuts at: 0 1015 1173
Size: 1015 158
ApoI r'AATT_y
Cuts at: 0 598 692 1173
Size: 598 94 481
Fragments arranged by size:
598 481 94
AseI AT'TA_AT
Cuts at: 0 443 1173
Size: 443 730
AvaI C'yCGr_G
Cuts at: 0 134 948 1173
Size: 134 814 225
Fragments arranged by size:
814 225 134
BanI G'GyrC_C
Cuts at: 0 13 1024 1173
Size: 13 1011 149
Fragments arranged by size:
1011 149 13
BanII G_rGCy'C
Cuts at: 0 196 1173
Size: 196 977
BbeI G_GCGC'C
Cuts at: 0 17 1173
Size: 17 1156
BfrBI ATG'CAT
Cuts at: 0 388 1173
Size: 388 785
BmrI ACTGGGnnnn_n'
Cuts at: 0 147 1173
Size: 147 1026
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 784 1173
Size: 694 90 389
Fragments arranged by size:
694 389 90
Bpu10I CC'TnA_GC
Cuts at: 0 724 1173
Size: 724 449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 1173
Size: 180 993
BsaHI Gr'CG_yC
Cuts at: 0 14 1173
Size: 14 1159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 151 181 1173
Size: 151 30 992
Fragments arranged by size:
992 151 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 152 175 1173
Size: 152 23 998
Fragments arranged by size:
998 152 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 976 1173
Size: 832 144 197
Fragments arranged by size:
832 197 144
BsiHKAI G_wGCw'C
Cuts at: 0 196 619 935 1173
Size: 196 423 316 238
Fragments arranged by size:
423 316 238 196
BsmBI CGTCTCn'nnnn_
Cuts at: 0 178 1173
Size: 178 995
Bsp1286I G_dGCh'C
Cuts at: 0 196 619 935 1173
Size: 196 423 316 238
Fragments arranged by size:
423 316 238 196
BspHI T'CATG_A
Cuts at: 0 1080 1173
Size: 1080 93
BsrDI GCAATG_nn'
Cuts at: 0 562 1173
Size: 562 611
BsrGI T'GTAC_A
Cuts at: 0 630 1173
Size: 630 543
BstXI CCAn_nnnn'nTGG
Cuts at: 0 912 1173
Size: 912 261
BtgI C'CryG_G
Cuts at: 0 905 1173
Size: 905 268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 932 1173
Size: 932 241
BtsI GCAGTG_nn'
Cuts at: 0 1092 1173
Size: 1092 81
ClaI AT'CG_AT
Cuts at: 0 755 1173
Size: 755 418
DraI TTT'AAA
Cuts at: 0 549 1173
Size: 549 624
EarI CTCTTCn'nnn_
Cuts at: 0 184 349 587 1153 1173
Size: 184 165 238 566 20
Fragments arranged by size:
566 238 184 165 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1059 1173
Size: 1059 114
EcoICRI GAG'CTC
Cuts at: 0 194 1173
Size: 194 979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 230 694 784 909 1173
Size: 76 154 464 90 125 264
Fragments arranged by size:
464 264 154 125 90 76
EcoO109I rG'GnC_Cy
Cuts at: 0 131 1173
Size: 131 1042
HaeII r_GCGC'y
Cuts at: 0 17 1173
Size: 17 1156
KasI G'GCGC_C
Cuts at: 0 13 1173
Size: 13 1160
MfeI C'AATT_G
Cuts at: 0 110 1173
Size: 110 1063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1160 1173
Size: 137 1023 13
Fragments arranged by size:
1023 137 13
MslI CAynn'nnrTG
Cuts at: 0 733 910 1085 1173
Size: 733 177 175 88
Fragments arranged by size:
733 177 175 88
MspA1I CmG'CkG
Cuts at: 0 1039 1173
Size: 1039 134
NarI GG'CG_CC
Cuts at: 0 14 1173
Size: 14 1159
NcoI C'CATG_G
Cuts at: 0 905 1173
Size: 905 268
NdeI CA'TA_TG
Cuts at: 0 824 1173
Size: 824 349
NsiI A_TGCA'T
Cuts at: 0 390 1173
Size: 390 783
NspI r_CATG'y
Cuts at: 0 732 1173
Size: 732 441
PfoI T'CCnGG_A
Cuts at: 0 671 1173
Size: 671 502
PpuMI rG'GwC_Cy
Cuts at: 0 131 1173
Size: 131 1042
PstI C_TGCA'G
Cuts at: 0 1038 1173
Size: 1038 135
PvuII CAG'CTG
Cuts at: 0 1039 1173
Size: 1039 134
SacI G_AGCT'C
Cuts at: 0 196 1173
Size: 196 977
SanDI GG'GwC_CC
Cuts at: 0 131 1173
Size: 131 1042
SapI GCTCTTCn'nnn_
Cuts at: 0 184 1173
Size: 184 989
SfcI C'TryA_G
Cuts at: 0 1034 1173
Size: 1034 139
SfoI GGC'GCC
Cuts at: 0 15 1173
Size: 15 1158
SmlI C'TyrA_G
Cuts at: 0 195 1173
Size: 195 978
SpeI A'CTAG_T
Cuts at: 0 811 1173
Size: 811 362
SspI AAT'ATT
Cuts at: 0 379 1173
Size: 379 794
StyI C'CwwG_G
Cuts at: 0 905 1173
Size: 905 268
TatI w'GTAC_w
Cuts at: 0 243 630 1173
Size: 243 387 543
Fragments arranged by size:
543 387 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1173
Size: 1117 32 24
Fragments arranged by size:
1117 32 24
XbaI T'CTAG_A
Cuts at: 0 885 1173
Size: 885 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 1173
Size: 525 648
XmnI GAAnn'nnTTC
Cuts at: 0 1088 1173
Size: 1088 85
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AseI AvaI BanI BanII
BbeI BfrBI BmrI BpmI Bpu10I BpuEI BsaHI BsaXI
BseRI BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrGI
BstXI BtgI BtgZI BtsI ClaI DraI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII KasI MfeI MmeI MslI
MspA1I NarI NcoI NdeI NsiI NspI PfoI PpuMI
PstI PvuII SacI SanDI SapI SfcI SfoI SmlI
SpeI SspI StyI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmI BspEI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I CspCI DraIII DrdI EaeI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MluI MscI NaeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI SwaI TaqII
Tth111I XhoI XmaI ZraI